{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T12:04:47Z","timestamp":1753877087326,"version":"3.41.2"},"reference-count":27,"publisher":"Oxford University Press (OUP)","issue":"9","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,9,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>The objective of this study was to quantify the differences in the activity of jejunal maltase and isomaltase between two groups of steers with average dry matter intake (DMI) and differing average daily gain (ADG). DMI and ADG were measured in crossbred steers (n = 69; initial body weight = 456 \u00b1 5.0 kg) consuming a finishing diet containing 67.8% dry-rolled corn, 20.0% wet distillers grains with solubles, 8.0% alfalfa hay, and 4.2% vitamin\/mineral supplement on a dry matter basis for 84 d. Jejunal mucosal samples were collected from eight steers with the greatest (high) or least (low) ADG and average DMI (\u00b1 0.55 standard deviation). Homogenates of jejunal mucosa were incubated with increasing amounts of maltose and isomaltose to determine the disaccharidase kinetics. Total mucosal protein concentration (mg protein\/g tissue; P = 0.45) of the mucosa and small intestinal weights (P = 0.69) did not differ between the groups. Neither the Michaelis\u2013Menten constant (Km) of isomaltase (P = 0.15) nor maltase (P = 0.21) differed between groups. The isomaltase maximum velocity (Vmax) expressed per gram of protein tended to differ (P = 0.10) between groups of steers but did not differ (P = 0.13) when expressed on a tissue basis. Similarly, neither the maltase Vmax expressed per gram of protein (P = 0.31) nor tissue (P = 0.32) differed between groups. While previous studies have indicated that disaccharidase expression is associated with differences in ADG, data presented here indicate that differences in enzyme activity at the end of the finishing period are minimal.<\/jats:p>","DOI":"10.1093\/jas\/skaa285","type":"journal-article","created":{"date-parts":[[2020,8,31]],"date-time":"2020-08-31T17:21:55Z","timestamp":1598894515000},"source":"Crossref","is-referenced-by-count":2,"title":["Associations of mucosal disaccharidase kinetics and expression in the jejunum of steers with divergent average daily gain"],"prefix":"10.1093","volume":"98","author":[{"given":"Wyatt N","family":"Smith","sequence":"first","affiliation":[{"name":"Department of Animal Science, South Dakota State University, Brookings, SD"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5564-8301","authenticated-orcid":false,"given":"Derek W","family":"Brake","sequence":"additional","affiliation":[{"name":"Department of Animal Science, South Dakota State University, Brookings, SD"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amanda K","family":"Lindholm-Perry","sequence":"additional","affiliation":[{"name":"U.S. Meat Animal Research Center, USDA, ARS, Clay Center, NE"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"William T","family":"Oliver","sequence":"additional","affiliation":[{"name":"U.S. Meat Animal Research Center, USDA, ARS, Clay Center, NE"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Harvey C","family":"Freetly","sequence":"additional","affiliation":[{"name":"U.S. Meat Animal Research Center, USDA, ARS, Clay Center, NE"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7995-1540","authenticated-orcid":false,"given":"Andrew P","family":"Foote","sequence":"additional","affiliation":[{"name":"U.S. Meat Animal Research Center, USDA, ARS, Clay Center, NE"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2020,8,29]]},"reference":[{"key":"2020092610243784900_CIT0001","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1002\/9781118392331.ch1","article-title":"Input factors affecting profitability: a changing paradigm and a challenging time","volume-title":"Feed efficiency in the beef industry","author":"Ahola","year":"2012"},{"key":"2020092610243784900_CIT0002","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1079\/BJN19710063","article-title":"A theoretical consideration of the effect of preventing rumen fermentation on the efficiency of utilization of dietary energy and protein in lambs","volume":"25","author":"Black","year":"1971","journal-title":"Br. J. Nutr"},{"key":"2020092610243784900_CIT0003","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1079\/BJN19730032","article-title":"Carbohydrases of the bovine small intestine","volume":"30","author":"Coombe","year":"1973","journal-title":"Br. J. Nutr"},{"key":"2020092610243784900_CIT0004","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1016\/0003-2697(64)90115-0","article-title":"Method for assay of intestinal disaccharidases","volume":"7","author":"Dahlqvist","year":"1964","journal-title":"Anal. Biochem"},{"key":"2020092610243784900_CIT0005","doi-asserted-by":"crossref","first-page":"5651","DOI":"10.2527\/jas.2014-8291","article-title":"Association of circulating active and total ghrelin concentrations with dry matter intake, growth, and carcass characteristics of finishing beef cattle","volume":"92","author":"Foote","year":"2014","journal-title":"J. Anim. Sci"},{"key":"2020092610243784900_CIT0006","doi-asserted-by":"crossref","first-page":"4430","DOI":"10.2527\/jas2017.1804","article-title":"Beef steers with average dry matter intake and divergent average daily gain have altered gene expression in the jejunum","volume":"95","author":"Foote","year":"2017","journal-title":"J. Anim. Sci"},{"key":"2020092610243784900_CIT0007","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1016\/j.domaniend.2015.12.007","article-title":"Leptin concentrations in finishing beef steers and heifers and their association with dry matter intake, average daily gain, feed efficiency, and body composition","volume":"55","author":"Foote","year":"2016","journal-title":"Domest. Anim. Endocrinol"},{"key":"2020092610243784900_CIT0008","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/0305-0491(89)90002-3","article-title":"Brush border membrane sucrase-isomaltase, maltase-glucoamylase and trehalase in mammals. Comparative development, effects of glucocorticoids, molecular mechanisms, and phylogenetic implications","volume":"94","author":"Galand","year":"1989","journal-title":"Comp. Biochem. Physiol. B"},{"key":"2020092610243784900_CIT0009","doi-asserted-by":"crossref","first-page":"172","DOI":"10.1093\/jn\/122.1.172","article-title":"Starch digestion and absorption in nonruminants","volume":"122","author":"Gray","year":"1992","journal-title":"J. Nutr"},{"key":"2020092610243784900_CIT0010","doi-asserted-by":"crossref","first-page":"915","DOI":"10.5713\/ajas.2009.r.08","article-title":"Understanding starch utilization in the small intestine of cattle","volume":"22","author":"Harmon","year":"2009","journal-title":"Asian-Australas. J. Anim. Sci"},{"key":"2020092610243784900_CIT0011","doi-asserted-by":"crossref","first-page":"852","DOI":"10.2527\/1997.753852x","article-title":"Starch utilization by ruminants: from basics to the bunk","volume":"75","author":"Huntington","year":"1997","journal-title":"J. Anim. Sci"},{"key":"2020092610243784900_CIT0012","doi-asserted-by":"crossref","first-page":"435","DOI":"10.1017\/S0021859600044142","article-title":"Carbohydrase activity in the pancreatic tissue and small intestine mucosa of sheep fed dried-grass or ground maize-based diets","volume":"104","author":"Janes","year":"1985","journal-title":"J. Agri. Sci"},{"key":"2020092610243784900_CIT0013","doi-asserted-by":"crossref","first-page":"2916","DOI":"10.2527\/1990.6892916x","article-title":"Influence of dietary forage and feed intake on carbohydrase activities and small intestinal morphology of calves","volume":"68","author":"Kreikemeier","year":"1990","journal-title":"J. Anim. Sci"},{"key":"2020092610243784900_CIT0014","doi-asserted-by":"crossref","first-page":"408","DOI":"10.1111\/age.12440","article-title":"Differential gene expression in the duodenum, jejunum and ileum among crossbred beef steers with divergent gain and feed intake phenotypes","volume":"47","author":"Lindholm-Perry","year":"2016","journal-title":"Anim. Genet"},{"key":"2020092610243784900_CIT0015","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method","volume":"25","author":"Livak","year":"2001","journal-title":"Methods"},{"key":"2020092610243784900_CIT0016","doi-asserted-by":"crossref","first-page":"3855","DOI":"10.2527\/jas.2014-7646","article-title":"Small intestinal growth measures are correlated with feed efficiency in market weight cattle, despite minimal effects of maternal nutrition during early to midgestation","volume":"92","author":"Meyer","year":"2014","journal-title":"J. Anim. Sci"},{"key":"2020092610243784900_CIT0017","doi-asserted-by":"crossref","first-page":"479","DOI":"10.1016\/j.cvfa.2008.06.002","article-title":"Surgery of the bovine large intestine","volume":"24","author":"Meylan","year":"2008","journal-title":"Vet. Clin. North Am. Food Anim. Pract"},{"key":"2020092610243784900_CIT0018","doi-asserted-by":"crossref","first-page":"1634","DOI":"10.2527\/jas1986.6351634x","article-title":"Limits to starch digestion in the ruminant small intestine","volume":"63","author":"Owens","year":"1986","journal-title":"J. Anim. Sci"},{"key":"2020092610243784900_CIT0019","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1097\/MPG.0b013e31804216fc","article-title":"Luminal substrate \u201cbrake\u201d on mucosal maltase-glucoamylase activity regulates total rate of starch digestion to glucose","volume":"45","author":"Quezada-Calvillo","year":"2007","journal-title":"J. Pediatr. Gastroenterol. Nutr"},{"key":"2020092610243784900_CIT0020","doi-asserted-by":"crossref","first-page":"447","DOI":"10.1111\/age.12546","article-title":"Relationships between inflammation- and immunity-related transcript abundance in the rumen and jejunum of beef steers with divergent average daily gain","volume":"48","author":"Reynolds","year":"2017","journal-title":"Anim. Genet"},{"key":"2020092610243784900_CIT0021","doi-asserted-by":"crossref","first-page":"1177","DOI":"10.2527\/jas1981.5251177x","article-title":"Effect dietary corn starch intake on pancreatic amylase and intestinal maltase and pH in cattle","volume":"52","author":"Russell","year":"1981","journal-title":"J. Anim. Sci"},{"key":"2020092610243784900_CIT0022","doi-asserted-by":"crossref","first-page":"839","DOI":"10.1042\/bj1080839","article-title":"Carbohydrase activities in the bovine digestive tract","volume":"108","author":"Siddons","year":"1968","journal-title":"Biochem. J"},{"key":"2020092610243784900_CIT0023","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1016\/0003-2697(85)90442-7","article-title":"Measurement of protein using bicinchoninic acid","volume":"150","author":"Smith","year":"1985","journal-title":"Anal. Biochem"},{"key":"2020092610243784900_CIT0024","doi-asserted-by":"crossref","first-page":"1781","DOI":"10.2527\/2004.8261781x","article-title":"Pancreatic exocrine secretion and plasma concentration of some gastrointestinal hormones in response to abomasal infusion of starch hydrolyzate and\/or casein","volume":"82","author":"Swanson","year":"2004","journal-title":"J. Anim. Sci"},{"key":"2020092610243784900_CIT0025","doi-asserted-by":"crossref","first-page":"1649","DOI":"10.2527\/jas1986.6351649x","article-title":"Grain processing effects on starch utilization by ruminants","volume":"63","author":"Theurer","year":"1986","journal-title":"J. Anim. Sci"},{"key":"2020092610243784900_CIT0026","doi-asserted-by":"crossref","first-page":"591","DOI":"10.1146\/annurev.ph.55.030193.003111","article-title":"Facilitated glucose transporters in epithelial cells","volume":"55","author":"Thorens","year":"1993","journal-title":"Annu. Rev. Physiol"},{"key":"2020092610243784900_CIT0027","doi-asserted-by":"crossref","first-page":"375","DOI":"10.5713\/ajas.1998.375","article-title":"Effects of nutritional level on digestive enzyme activities in the pancreas and small intestine of calves slaughtered at same body weight","volume":"11","author":"Wang","year":"1998","journal-title":"Asian-Australas. J. Anim. Sci"}],"container-title":["Journal of Animal Science"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/academic.oup.com\/jas\/advance-article-pdf\/doi\/10.1093\/jas\/skaa285\/33697328\/skaa285.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"http:\/\/academic.oup.com\/jas\/article-pdf\/98\/9\/skaa285\/33795658\/skaa285.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"http:\/\/academic.oup.com\/jas\/article-pdf\/98\/9\/skaa285\/33795658\/skaa285.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,9,26]],"date-time":"2020-09-26T14:24:48Z","timestamp":1601130288000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jas\/article\/doi\/10.1093\/jas\/skaa285\/5899103"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,8,29]]},"references-count":27,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2020,8,29]]},"published-print":{"date-parts":[[2020,9,1]]}},"URL":"https:\/\/doi.org\/10.1093\/jas\/skaa285","relation":{},"ISSN":["0021-8812","1525-3163"],"issn-type":[{"type":"print","value":"0021-8812"},{"type":"electronic","value":"1525-3163"}],"subject":[],"published-other":{"date-parts":[[2020,9]]},"published":{"date-parts":[[2020,8,29]]},"article-number":"skaa285"}}