{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T19:23:46Z","timestamp":1791919426737,"version":"4.3.4"},"reference-count":60,"publisher":"Oxford University Press (OUP)","issue":"9","license":[{"start":{"date-parts":[[2020,9,1]],"date-time":"2020-09-01T00:00:00Z","timestamp":1598918400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/100008370","name":"National Pork Board","doi-asserted-by":"publisher","award":["#18-081"],"award-info":[{"award-number":["#18-081"]}],"id":[{"id":"10.13039\/100008370","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Animal Health Formula Funds","award":["CALV-AH-045"],"award-info":[{"award-number":["CALV-AH-045"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,9,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The study was conducted to investigate the efficacy of a probiotic Bacillus subtilis strain on growth performance, diarrhea, systemic immunity, and intestinal health of weaned pigs experimentally infected with an enterotoxigenic Escherichia coli and to compare the efficacy of B. subtilis with that of carbadox. Weaned pigs (n = 48, 6.17 \u00b1 0.36 kg body weight [BW]) were individually housed in disease containment rooms and randomly allotted to one of four dietary treatments: negative control (NC, control diet without E. coli challenge), positive control (PC, control diet with E. coli challenge), and supplementation of 50 mg\/kg of carbadox (antibiotic growth promotor [AGP]) or 2.56 \u00d7 109 CFU\/kg of B. subtilis probiotics (PRO). The experiment lasted for 28 d with 7 d before and 21 d after the first E. coli inoculation. Fecal and blood samples were collected on days 0, 3, 7, 14, and 21 post inoculation (PI) to analyze \u03b2-hemolytic coliforms and complete blood cell count, respectively. Diarrhea score was recorded daily for each pig to calculate the frequency of diarrhea. All pigs were euthanized at day 21 PI to collect jejunal and ileal mucosa for gene expression analysis. Pigs in AGP had greater (P &amp;lt; 0.05) BW on days 7, 14, and 21 PI than pigs in PC and PRO groups. Supplementation of PRO enhanced pigs\u2019 BW on day 21 PI compared with the PC. Escherichia coli F18 challenge reduced (P &amp;lt; 0.05) average daily gain (ADG) and feed efficiency from day 0 to 21 PI, while supplementation of carbadox or PRO enhanced ADG and feed efficiency in E. coli F18-challenged pigs from day 0 to 21 PI. Pigs in AGP and PRO groups had reduced (P &amp;lt; 0.05) frequency of diarrhea throughout the experiment and fecal \u03b2-hemolytic coliforms on day 7 PI than pigs in the PC. Pigs in PRO had greater (P &amp;lt; 0.05) gene expression of CLDN1 in jejunal mucosa than pigs in the PC. Supplementation of carbadox or PRO reduced (P &amp;lt; 0.05) the gene expression of IL6 and PTGS2 in ileal mucosa of E. coli-infected pigs compared with pigs in the PC. Pigs in the PRO group had lower (P &amp;lt; 0.05) white blood cell number and neutrophil count, and serum haptoglobin concentration on day 7 PI, and less (P &amp;lt; 0.05) monocyte count on day 14 PI, compared with PC. In conclusion, supplementation of probiotic B. subtilis could enhance disease resistance and promote the growth performance of weaned pigs under disease challenge conditions. The potential mechanisms include but not limited to enhanced gut barrier integrity and local and systemic immune responses of weaned pigs.<\/jats:p>","DOI":"10.1093\/jas\/skaa290","type":"journal-article","created":{"date-parts":[[2020,9,2]],"date-time":"2020-09-02T15:31:47Z","timestamp":1599060707000},"source":"Crossref","is-referenced-by-count":39,"title":["Bacillus subtilis: a potential growth promoter in weaned pigs in comparison to carbadox"],"prefix":"10.1093","volume":"98","author":[{"given":"Yijie","family":"He","sequence":"first","affiliation":[{"name":"Department of Animal Science, University of California, Davis, CA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kwangwook","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Animal Science, University of California, Davis, CA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lauren","family":"Kovanda","sequence":"additional","affiliation":[{"name":"Department of Animal Science, University of California, Davis, CA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cynthia","family":"Jinno","sequence":"additional","affiliation":[{"name":"Department of Animal Science, University of California, Davis, CA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Minho","family":"Song","sequence":"additional","affiliation":[{"name":"Department of Animal Science and Biotechnology, Chungnam National University, Daejeon, South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer","family":"Chase","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine, University of California, Davis, CA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xunde","family":"Li","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine, University of California, Davis, CA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0138-1530","authenticated-orcid":false,"given":"Bie","family":"Tan","sequence":"additional","affiliation":[{"name":"Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanhong","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Animal Science, University of California, Davis, CA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2020,9,2]]},"reference":[{"key":"2020092910544669600_CIT0001","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1146\/annurev-micro-091213-113052","article-title":"Altered egos: antibiotic effects on food animal microbiomes","volume":"68","author":"Allen","year":"2014","journal-title":"Annu. 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