{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T19:31:44Z","timestamp":1789241504136,"version":"build-2803163510"},"reference-count":46,"publisher":"Oxford University Press (OUP)","issue":"10","license":[{"start":{"date-parts":[[2020,9,14]],"date-time":"2020-09-14T00:00:00Z","timestamp":1600041600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"name":"DSM Nutritional Products"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,10,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Two experiments were conducted to determine the effects of protease supplementation on degradation of soybean meal (SBM) allergenic proteins (glycinin and \u03b2-conglycinin) and gut health of weaned pigs fed soybean meal-based diets. In experiment 1, 2 SBM samples from 2 different sources were subjected to porcine in vitro gastric degradation to determine the effects of protease (at 15,000 U\/kg of feedstuff) on degradation of the soybean allergenic proteins. In experiment 2, 48 weaned pigs (body weight = 6.66 kg) were obtained in 2 batches of 24 pigs each. Pigs were individually housed in metabolic crates and fed 4 diets (12 pigs\/diet). The diets were corn-based diet with SBM 1 or SBM 2 without or with protease at 15,000 U\/kg of diet in 2 \u00d7 2 factorial arrangement. Diets were fed for 10 d and pigs were sacrificed on day 10 for measurement of small intestinal histomorphology, permeability of small intestine mounted in Ussing chambers, and serum concentration of pro-inflammatory cytokines. Two SBM sources (SBM 1 and SBM 2) contained 46.9% or 47.7% CP, 14.0% or 14.6% glycinin, and 9.90% or 10.3% \u03b2-conglycinin, respectively. Protease and SBM source did not interact on any of the response criteria measured in the current study. Protease supplementation tended to increase (P = 0.069) the in vitro gastric degradation of glycinin. Protease supplementation tended to reduce (P = 0.099) fluorescein isothiocyanate dextran 4,000 Da (which is a marker probe for intestinal permeability) flow in jejunum, and reduced (P = 0.037) serum TNF-\u03b1 concentration. Protease did not affect small intestinal histomorphology. In conclusion, protease tended to increase gastric degradation of glycinin and reduce gut permeability, and serum concentration of pro-inflammatory cytokines, indicating that the protease used in the current study can be added to SBM-based diets for weanling pigs to improve gut health.<\/jats:p>","DOI":"10.1093\/jas\/skaa308","type":"journal-article","created":{"date-parts":[[2020,9,14]],"date-time":"2020-09-14T21:24:13Z","timestamp":1600118653000},"source":"Crossref","is-referenced-by-count":28,"title":["Soybean meal allergenic protein degradation and gut health of piglets fed protease-supplemented diets"],"prefix":"10.1093","volume":"98","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2288-1374","authenticated-orcid":false,"given":"Sangwoo","family":"Park","sequence":"first","affiliation":[{"name":"Department of Animal Science, South Dakota State University, Brookings, SD"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jung Wook","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Animal Science, South Dakota State University, Brookings, SD"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aaron J","family":"Cowieson","sequence":"additional","affiliation":[{"name":"DSM Nutritional Products, Wurmisweg, Kaiseraugst, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guenter","family":"Pappenberger","sequence":"additional","affiliation":[{"name":"DSM Nutritional Products, Wurmisweg, Kaiseraugst, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tofuko Awori","family":"Woyengo","sequence":"additional","affiliation":[{"name":"Department of Animal Science, South Dakota State University, Brookings, SD"},{"name":"Department of Animal Science, Aarhus University, Blichers All\u00e9, Tjele, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2020,9,14]]},"reference":[{"key":"2020101708245027000_CIT0001","volume-title":"Official methods of analysis","author":"AOAC.","year":"2012","edition":"19th ed."},{"key":"2020101708245027000_CIT0002","doi-asserted-by":"crossref","first-page":"1272","DOI":"10.3168\/jds.S0022-0302(83)81934-1","article-title":"Relationship between electrophoretic band patterns and in vitro ammonia release of soluble soybean meal protein","volume":"66","author":"van der Aar","year":"1982","journal-title":"J. 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