{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,6]],"date-time":"2026-06-06T04:15:42Z","timestamp":1780719342771,"version":"3.54.1"},"reference-count":41,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2021,1,1]],"date-time":"2021-01-01T00:00:00Z","timestamp":1609459200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"name":"Research Foundation of Education Bureau of Hunan Province","award":["18B476"],"award-info":[{"award-number":["18B476"]}]},{"DOI":"10.13039\/501100004735","name":"Hunan Provincial Natural Science Foundation of China","doi-asserted-by":"crossref","award":["2018JJ3094"],"award-info":[{"award-number":["2018JJ3094"]}],"id":[{"id":"10.13039\/501100004735","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,1,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Folate is increasingly thought to promote gastrointestinal health and regulate the diversity of gut microbiota to alleviate weaning stress in piglets. The present study was conducted to investigate the effects of folate on organ weight, digesta pH, short-chain fatty acids (SCFAs) concentration, and intestinal microbiota in weaned piglets. A total of 28 piglets (6.73 \u00b1 0.62 kg) were allocated to four dietary treatments consisting of a control group, 3, 9, and 18 mg\/kg of folate supplementation in a 14-d feeding trial. The results showed that piglets fed with 9 and 18 mg\/kg of folate supplementation had greater (P &amp;lt; 0.05) average liver and spleen weight than the control group. Folate supplementation (9 and 18 mg\/kg) can significantly increase (P &amp;lt; 0.05) the stomach pH and tend (P &amp;lt; 0.10) to decrease the cecum pH. Folate treatment (9 and 18 mg\/kg) had a positive effect on the metabolism of SCFAs in piglets, in particular, compared with the control group, and the content of acetic acid (AA) and valeric acid was markedly increased (P &amp;lt; 0.05) in the cecum and colon, respectively. Moreover, isobutyric acid, butyric acid, and isovaleric acid were tended (P &amp;lt; 0.10) to increase in the colon. Cecum contents samples were used to determine bacterial community diversity by 16S rRNA gene amplicon sequencing. At the genus level, in the cecum, there was a higher (P &amp;lt; 0.05) relative abundance of Lactobacillus reuteri, Lactobacillus salivarius, and Lactobacillus mucosae in the 9 mg\/kg folate supplementation group. The functional pathways analysis predicted that folate may modify nutrient metabolism by changing the gut microbiota function of weaned piglets. Furthermore, the data showed that Lactobacillus was positively correlated with AA in the cecum. Overall, these findings suggested that folate treatment could increase the organ weight and the stomach pH of weaned piglets and had beneficial effects on gut health, which might be attributed to the alteration in intestinal microbiota induced by folate and the interaction of the intestinal microbiota with SCFAs.<\/jats:p>","DOI":"10.1093\/jas\/skab015","type":"journal-article","created":{"date-parts":[[2021,1,21]],"date-time":"2021-01-21T17:58:50Z","timestamp":1611251930000},"source":"Crossref","is-referenced-by-count":27,"title":["Effect of dietary folate level on organ weight, digesta pH, short-chain fatty acid concentration, and intestinal microbiota of weaned piglets"],"prefix":"10.1093","volume":"99","author":[{"given":"Lei","family":"Wang","sequence":"first","affiliation":[{"name":"Hunan International Joint Laboratory of Animal Intestinal Ecology and Health, School of Life Sciences, Hunan Normal University, Changsha, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lijun","family":"Zou","sequence":"additional","affiliation":[{"name":"Laboratory of Basic Biology, Hunan First Normal University, Changsha, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianzhong","family":"Li","sequence":"additional","affiliation":[{"name":"Hunan International Joint Laboratory of Animal Intestinal Ecology and Health, School of Life Sciences, Hunan Normal University, Changsha, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1164-5771","authenticated-orcid":false,"given":"Huansheng","family":"Yang","sequence":"additional","affiliation":[{"name":"Hunan International Joint Laboratory of Animal Intestinal Ecology and Health, School of Life Sciences, Hunan Normal University, Changsha, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yulong","family":"Yin","sequence":"additional","affiliation":[{"name":"Hunan International Joint Laboratory of Animal Intestinal Ecology and Health, School of Life Sciences, Hunan Normal University, Changsha, China"},{"name":"Hunan Province Key Laboratory of Animal Nutritional Physiology and Metabolic Process, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2021,1,21]]},"reference":[{"key":"2021020211453830500_CIT0001","doi-asserted-by":"crossref","first-page":"797","DOI":"10.1016\/j.clnu.2017.03.025","article-title":"A randomized trial to determine the impact of a digestion resistant starch composition on the gut microbiome in older and mid-age adults","volume":"37","author":"Alfa","year":"2018","journal-title":"Clin. 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