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Therefore, the objectives of this study were to determine whether gestational age, endogenous progesterone, maternal nutrient restriction, or dietary melatonin altered the composition of the bovine vaginal microbiota. Brangus heifers (n = 29) from timed artificial insemination to day 240 of gestation were used; at day 160 of gestation, heifers were assigned to either an adequate (ADQ; n = 14; 100% NRC requirements) or restricted (RES; n = 15; 60% NRC requirements) nutritional plane and were either supplemented with dietary melatonin (MEL; n = 15) or not supplemented (CON; n = 14). Samples for vaginal microbiota analysis were taken on day 0 (prior to artificial insemination), day 150 (prior to dietary treatments), and day 220 of gestation (60 d post-treatment initiation) using a double guarded culture swab. The vaginal bacterial overall community structure was determined through sequencing the V4 region of the 16S rRNA gene using the Illumina Miseq platform. Alpha diversity was compared via 2-way ANOVA; \u03b2 diversity was compared via PERMANOVA. The linear discriminant analysis for effect size (LEfSe) pipeline was utilized for analysis of taxonomic rank differences between bacterial communities. Gestational age, progesterone concentration, and maternal nutritional plane did not alter \u03b1 or \u03b2 diversity of the vaginal microbiota. However, gestational age resulted in compositional changes at the order, family, and genus level. Moreover, dietary melatonin supplementation did not alter \u03b1 diversity of the vaginal microbiota but did alter \u03b2 diversity (P = 0.02). Specifically, melatonin altered the composition at the genus level and increased the prevalence of aerobic bacteria in the vaginal tract. To date, melatonin is the first hormone associated with altering the composition of the bovine vaginal microbiota.<\/jats:p>","DOI":"10.1093\/jas\/skab098","type":"journal-article","created":{"date-parts":[[2021,3,27]],"date-time":"2021-03-27T22:06:05Z","timestamp":1616882765000},"source":"Crossref","is-referenced-by-count":9,"title":["Melatonin-induced changes in the bovine vaginal microbiota during maternal nutrient restriction"],"prefix":"10.1093","volume":"99","author":[{"given":"Riley D","family":"Messman","sequence":"first","affiliation":[{"name":"Department of Animal and Dairy Sciences, Mississippi State University, Mississippi State, MS, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zully E","family":"Contreras-Correa","sequence":"additional","affiliation":[{"name":"Department of Animal and Dairy Sciences, Mississippi State University, Mississippi State, MS, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Henry A","family":"Paz","sequence":"additional","affiliation":[{"name":"Department of Animal and Dairy Sciences, Mississippi State University, Mississippi State, MS, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1377-0664","authenticated-orcid":false,"given":"Caleb O","family":"Lemley","sequence":"additional","affiliation":[{"name":"Department of Animal and Dairy Sciences, Mississippi State University, Mississippi State, MS, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2021,3,27]]},"reference":[{"key":"2022032913350546400_CIT0001","doi-asserted-by":"crossref","first-page":"e10437.","DOI":"10.1038\/s41598-018-28733-y","article-title":"The composition of the perinatal intestinal microbiota in cattle","volume":"8","author":"Alipour","year":"2018","journal-title":"Sci Rep"},{"key":"2022032913350546400_CIT0002","doi-asserted-by":"crossref","first-page":"4298","DOI":"10.1093\/jas\/skz210","article-title":"Uterine and vaginal bacterial community diversity prior to artificial insemination between pregnant and nonpregnant postpartum cows1","volume":"97","author":"Ault","year":"2019","journal-title":"J. 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