{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,22]],"date-time":"2026-01-22T11:37:11Z","timestamp":1769081831626,"version":"3.49.0"},"reference-count":33,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2021,4,17]],"date-time":"2021-04-17T00:00:00Z","timestamp":1618617600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,6,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Dietary and microbially derived fatty acids (FA) play important roles in gut mucosal inflammatory signaling, barrier function, and oxidative stress response. Nevertheless, little information is available about gastrointestinal FA profiles and receptor distribution in pigs, especially for long-chain FA (LCFA). Therefore, the present pilot study aimed to (1) investigate the gastrointestinal FA profiles; (2) link the luminal FA profiles to the mucosal expression of genes related to FA sensing and signaling; and (3) assess potential dietary effects on gut and systemic lipid metabolism in pigs. Gut, liver, and serum samples were obtained from barrows (13.1 \u00b1 2.3 kg) fed diets containing either phytase (500 phytase units\/kg diet) or cereals treated with 2.5% lactic acid (LA; n = 8\/diet) for 18 d. Results showed gut regional and diet-related differences in luminal FA profiles and mucosal receptor expression, whereas diet little affected hepatic expression levels and serum lipids. Short-chain fatty acids (SCFA) increased from stomach, jejunum, and ileum to the cecum (P &amp;lt; 0.05), whereas LCFA were higher in stomach, cecum, and colon than in jejunum and ileum (P &amp;lt; 0.05). LA-treated cereals enhanced cecal acetate and butyrate, whereas phytase and LA treated cereals decreased the LCFA by 35.9% and 14.4%, respectively (P &amp;lt; 0.05). Gut regional differences suggested stronger signaling via FFAR1 expression in the ileum, and via FFAR2, FFAR4, and HCAR1 expression in cecum and colon (P &amp;lt; 0.05). Expression of AMPK, FASN, PPARG, SREBP1, and SREBP2 was higher in the cecum and colon compared with the small intestine (P &amp;lt; 0.05), with stronger sensing via FASN and SREBP2. Phytase decreased expression of FFAR2 and FFAR4, whereas it increased that of FFAR3 and MCT1 in the cecum (P &amp;lt; 0.05). LA-treated cereals raised cecal expression of FFAR3 and HCAR1 (P &amp;lt; 0.05). Pearson\u2019s correlations (|r| &amp;gt; 0.35; P &amp;lt; 0.05) supported that FA receptor- and nuclear transcription factor-dependent pathways were involved in the mucosal regulation of gut incretin expression but differed across gut regions. In conclusion, results support regional differences in SCFA, lactate and LCFA sensing and absorption capacities in the small and large intestines of pigs. Effects of phytase and the LA-treated cereals on intestinal FA levels and signaling can be explained by differences in nutrient flows (e.g., phosphorus and carbohydrate fractions). This overview provides a solid basis for future intestinal FA sensing in pigs.<\/jats:p>","DOI":"10.1093\/jas\/skab117","type":"journal-article","created":{"date-parts":[[2021,4,17]],"date-time":"2021-04-17T12:04:30Z","timestamp":1618661070000},"source":"Crossref","is-referenced-by-count":18,"title":["Short-, medium-, and long-chain fatty acid profiles and signaling is responsive to dietary phytase and lactic acid treatment of cereals along the gastrointestinal tract of growing pigs"],"prefix":"10.1093","volume":"99","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2413-4957","authenticated-orcid":false,"given":"Barbara U","family":"Metzler-Zebeli","sequence":"first","affiliation":[{"name":"Unit Nutritional Physiology, Institute of Physiology, Pathophysiology and Biophysics, Department of Biomedical Sciences, University of Veterinary Medicine Vienna, 1210 Vienna, Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jutamat","family":"Klinsoda","sequence":"additional","affiliation":[{"name":"Unit Nutritional Physiology, Institute of Physiology, Pathophysiology and Biophysics, Department of Biomedical Sciences, University of Veterinary Medicine Vienna, 1210 Vienna, Austria"},{"name":"Institute of Food Research and Product Development, University of Kasetsart, Bangkok, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julia","family":"V\u00f6tterl","sequence":"additional","affiliation":[{"name":"Unit Nutritional Physiology, Institute of Physiology, Pathophysiology and Biophysics, Department of Biomedical Sciences, University of Veterinary Medicine Vienna, 1210 Vienna, Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Suchitra","family":"Sharma","sequence":"additional","affiliation":[{"name":"Institute of Animal Nutrition and Functional Plant Compounds, Department for Farm Animals and Veterinary Public Health, University of Veterinary Medicine Vienna, 1210 Vienna, Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Simone","family":"Koger","sequence":"additional","affiliation":[{"name":"Institute of Food Research and Product Development, University of Kasetsart, Bangkok, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Arife","family":"Sener-Aydemir","sequence":"additional","affiliation":[{"name":"Institute of Food Research and Product Development, University of Kasetsart, Bangkok, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2021,4,17]]},"reference":[{"key":"2021070809371173500_CIT0001","doi-asserted-by":"crossref","DOI":"10.5772\/intechopen.70653","article-title":"Chapter 8: metabolic processes during seed germination.","volume-title":"Advances in seed biology","author":"Ali","year":"2017"},{"key":"2021070809371173500_CIT0002","doi-asserted-by":"crossref","first-page":"489","DOI":"10.1038\/s41589-019-0270-1","article-title":"Chemogenetics defines receptor-mediated functions of short chain free fatty acids","volume":"15","author":"Bolognini","year":"2019","journal-title":"Nat. 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