{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T16:17:04Z","timestamp":1785428224627,"version":"3.56.0"},"reference-count":87,"publisher":"Oxford University Press (OUP)","issue":"7","license":[{"start":{"date-parts":[[2021,6,14]],"date-time":"2021-06-14T00:00:00Z","timestamp":1623628800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000199","name":"U.S. Department of Agriculture","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000199","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100005825","name":"National Institute of Food and Agriculture","doi-asserted-by":"publisher","award":["02636"],"award-info":[{"award-number":["02636"]}],"id":[{"id":"10.13039\/100005825","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Huvepharma Inc"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,7,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>This study aimed to evaluate the effects of increasing levels of \u03b2-glucanase on the modulation of jejunal mucosa-associated microbiota in relation to nutrient digestibility and intestinal health of pigs fed diets with 30% corn distiller\u2019s dried grains with solubles and xylanase. Forty pigs at 12.4 \u00b1 0.5 kg body weight (BW) were allotted in a randomized complete block design with initial BW and sex as blocks. Dietary treatments consisted of a basal diet with xylanase (1,500 endo-pentosanase units [EPU]\/kg) and increasing levels of \u03b2-glucanase (0, 200, 400, and 600 U\/kg) meeting nutrient requirements and fed to pigs for 21 d. Blood samples were collected on day 19. On day 21, all pigs were euthanized to collect intestinal tissues and digesta. Tumor necrosis factor-alpha, interleukin (IL)-6, and malondialdehyde were measured in the plasma and mid-jejunal mucosa. Viscosity was determined using digesta from the distal jejunum. Ileal and rectal digesta were evaluated to determine apparent ileal digestibility (AID) and apparent total tract digestibility (ATTD) of nutrients. Mucosa samples from the mid-jejunum were utilized for microbiota sequencing. Data were analyzed using the MIXED procedure on SAS 9.4. Overall, increasing dietary \u03b2-glucanase tended to increase (linear; P = 0.077) the average daily gain of pigs. Increasing dietary \u03b2-glucanase affected (quadratic; P &amp;lt; 0.05) the relative abundance of Bacteroidetes, reduced (linear; P &amp;lt; 0.05) Helicobacter rappini, and increased (linear, P &amp;lt; 0.05) Faecalibacterium prausnitzii. \u03b2-Glucanase supplementation (0 vs. others) tended to increase (P = 0.096) the AID of crude protein in the diet, whereas increasing dietary \u03b2-glucanase tended to increase (linear; P = 0.097) the ATTD of gross energy in the diet and increased (linear; P &amp;lt; 0.05) the concentration of IL-6 in the plasma of pigs. In conclusion, increasing \u03b2-glucanase up to 600 U\/kg feed in a diet containing xylanase (1,500 EPU\/kg) modulated mucosa-associated microbiota by increasing the relative abundance of beneficial bacteria and reducing potentially harmful bacteria. Furthermore, increasing \u03b2-glucanase up to 600 U\/kg feed in a diet containing xylanase (1,500 EPU\/kg feed) enhanced the status of the intestinal environment and nutrient utilization, as well as reduced systemic inflammation of pigs, collectively resulting in moderate improvement of growth performance. Supplementing \u03b2-glucanase at a range of 312 to 410 U\/kg with xylanase at 1,500 EPU\/kg feed showed the most benefit on jejunal mucosa-associated microbiota and reduced systemic inflammation of pigs.<\/jats:p>","DOI":"10.1093\/jas\/skab190","type":"journal-article","created":{"date-parts":[[2021,6,14]],"date-time":"2021-06-14T14:31:41Z","timestamp":1623681101000},"source":"Crossref","is-referenced-by-count":31,"title":["Modulation of jejunal mucosa-associated microbiota in relation to intestinal health and nutrient digestibility in pigs by supplementation of \u03b2-glucanase to corn\u2013soybean meal-based diets with xylanase"],"prefix":"10.1093","volume":"99","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0094-336X","authenticated-orcid":false,"given":"Marcos Elias","family":"Duarte","sequence":"first","affiliation":[{"name":"Department of Animal Science, North Carolina State University, Raleigh, NC 27695, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chris","family":"Sparks","sequence":"additional","affiliation":[{"name":"Huvepharma, Inc., Peachtree City, GA, 30269, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4591-1943","authenticated-orcid":false,"given":"Sung Woo","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Animal Science, North Carolina State University, Raleigh, NC 27695, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2021,6,14]]},"reference":[{"key":"2021072108593136300_CIT0001","doi-asserted-by":"crossref","first-page":"3189","DOI":"10.2527\/jas.2010-3715","article-title":"Opportunities and challenges in using exogenous enzymes to improve nonruminant animal production","volume":"89","author":"Adeola","year":"2011","journal-title":"J. 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