{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T22:10:22Z","timestamp":1792620622506,"version":"4.5.0"},"reference-count":45,"publisher":"Oxford University Press (OUP)","issue":"8","license":[{"start":{"date-parts":[[2021,7,29]],"date-time":"2021-07-29T00:00:00Z","timestamp":1627516800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/100000199","name":"U.S. Department of Agriculture","doi-asserted-by":"publisher","award":["USDA-2016-67015-24582"],"award-info":[{"award-number":["USDA-2016-67015-24582"]}],"id":[{"id":"10.13039\/100000199","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["DGE-1650441"],"award-info":[{"award-number":["DGE-1650441"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,8,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The fungal isolate myriocin inhibits serine palmitoyltransferase and de novo ceramide synthesis in rodents; however, the effects of myriocin on ceramide concentrations and metabolism have not been previously investigated in ruminants. In our study, 12 non-lactating crossbred ewes received an intravenous bolus of myriocin (0, 0.1, 0.3, or 1.0 mg\/kg\/body weight [BW]; CON, LOW, MOD, or HIGH) every 48 h for 17 d. Ewes consumed a high-energy diet from day 1 to 14 and were nutrient-restricted (straw only) from day 15 to 17. Blood was collected preprandial and at 1, 6, and 12 h relative to bolus and nutrient restriction. Tissues were collected following euthanasia on day 17. Plasma was analyzed for free fatty acids (FFAs), glucose, and insulin. Plasma and tissue ceramides were quantified using mass spectrometry. HIGH selectively decreased metabolizable energy intake, BW, and plasma insulin, and increased plasma FFA (Dose, P &amp;lt; 0.05). Myriocin linearly decreased plasma very-long-chain (VLC) ceramide and dihydroceramide (DHCer) by day 13 (Linear, P &amp;lt; 0.05). During nutrient restriction, fold-change in FFA was lower with increasing dose (P &amp;lt; 0.05). Nutrient restriction increased plasma C16:0-Cer, an effect suppressed by MOD and HIGH (Dose \u00d7 Time, P &amp;lt; 0.05). Myriocin linearly decreased most ceramide and DHCer species in the liver and omental and mesenteric adipose, VLC ceramide and DHCer in the pancreas, and C18:0-Cer in skeletal muscle and subcutaneous adipose tissue (Linear, P \u2264 0.05). We conclude that the intravenous delivery of 0.3 mg of myriocin\/kg of BW\/48 h decreases circulating and tissue ceramide without modifying energy intake in ruminants.<\/jats:p>","DOI":"10.1093\/jas\/skab221","type":"journal-article","created":{"date-parts":[[2021,7,29]],"date-time":"2021-07-29T17:00:46Z","timestamp":1627578046000},"source":"Crossref","is-referenced-by-count":6,"title":["Effects of serine palmitoyltransferase inhibition by myriocin in ad libitum-fed and nutrient-restricted ewes"],"prefix":"10.1093","volume":"99","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6370-3075","authenticated-orcid":false,"given":"Amanda N","family":"Davis","sequence":"first","affiliation":[{"name":"Department of Animal Science, Cornell University , Ithaca, NY 14853 , USA"},{"name":"Biological Sciences Department, State University of New York College at Cortland , Cortland, NY 13045 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William A","family":"Myers","sequence":"additional","affiliation":[{"name":"Department of Animal Science, Cornell University , Ithaca, NY 14853 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jorge","family":"Eduardo Rico","sequence":"additional","affiliation":[{"name":"Department of Animal Science, Cornell University , Ithaca, NY 14853 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lin","family":"Feng Wang","sequence":"additional","affiliation":[{"name":"Department of Animal Science, Cornell University , Ithaca, NY 14853 , USA"},{"name":"College of Animal Science and Veterinary Medicine, Henan Agricultural University , Zengzhou 450002 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Crystal","family":"Chang","sequence":"additional","affiliation":[{"name":"Department of Animal Science, Cornell University , Ithaca, NY 14853 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew T","family":"Richards","sequence":"additional","affiliation":[{"name":"Department of Animal Science, Cornell University , Ithaca, NY 14853 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammed","family":"Moniruzzaman","sequence":"additional","affiliation":[{"name":"Department of Neurology, Johns Hopkins University School of Medicine , Baltimore, MD 21287 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Norman J","family":"Haughey","sequence":"additional","affiliation":[{"name":"Department of Neurology, Johns Hopkins University School of Medicine , Baltimore, MD 21287 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5147-9013","authenticated-orcid":false,"given":"Joseph W","family":"McFadden","sequence":"additional","affiliation":[{"name":"Department of Animal Science, Cornell University , Ithaca, NY 14853 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2021,7,29]]},"reference":[{"key":"2022072900100206000_CIT0001","doi-asserted-by":"crossref","first-page":"25","DOI":"10.2337\/diabetes.53.1.25","article-title":"Ceramide content is increased in skeletal muscle from obese insulin-resistant humans","volume":"53","author":"Adams","year":"2004","journal-title":"Diabetes"},{"key":"2022072900100206000_CIT0002","doi-asserted-by":"crossref","first-page":"869","DOI":"10.1002\/iub.400","article-title":"Gluconeogenesis in dairy cows: the secret of making sweet milk from sour dough","volume":"62","author":"Aschenbach","year":"2010","journal-title":"IUBMB Life"},{"key":"2022072900100206000_CIT0003","doi-asserted-by":"crossref","first-page":"1282","DOI":"10.3168\/jds.S0022-0302(71)86021-6","article-title":"Propionate production in the rumen of cows fed either a control or high-grain, low-fiber diet","volume":"54","author":"Bauman","year":"1971","journal-title":"J. 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