{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T11:23:08Z","timestamp":1768476188978,"version":"3.49.0"},"reference-count":52,"publisher":"Oxford University Press (OUP)","issue":"10","license":[{"start":{"date-parts":[[2021,8,26]],"date-time":"2021-08-26T00:00:00Z","timestamp":1629936000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32172924"],"award-info":[{"award-number":["32172924"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31873029"],"award-info":[{"award-number":["31873029"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31502136"],"award-info":[{"award-number":["31502136"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Project of Modern Agricultural Industry and Technology System of Anhui Province","award":["AHCYJSTX-07"],"award-info":[{"award-number":["AHCYJSTX-07"]}]},{"name":"Key Research and Development Plan Projects of Anhui Province","award":["1804g07020187"],"award-info":[{"award-number":["1804g07020187"]}]},{"name":"Key Research and Development Plan Projects of Anhui Province","award":["1804g07020188"],"award-info":[{"award-number":["1804g07020188"]}]},{"name":"Agricultural Industry Chief Expert Studio of Hefei City"},{"DOI":"10.13039\/501100004543","name":"China Scholarship Council","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004543","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,10,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Sirtuin 1 (SIRT1), an NAD-dependent protein deacetylase, plays a central role in the control of lipid metabolism in nonruminants. However, the role of SIRT1 in hepatic lipid metabolism in dairy cows with fatty liver is not well known. Thus, we used isolated primary bovine hepatocytes to determine the role of SIRT1 in protecting cells against oleic acid (OA)-induced steatosis. Recombinant adenoviruses to overexpress (AD-GFP-SIRT1-E) or knockdown (AD-GFP-SIRT1-N) SIRT1 were used for transduction of hepatocytes. Calf hepatocytes isolated from five female calves (1 d old, 30 to 40 kg) were used to determine both time required and the lowest dose of OA that could induce triacylglycerol (TAG) accumulation. Analyses indicated that 0.25 mM OA for 24 h was suitable to induce TAG accumulation. In addition, OA not only led to an increase in TAG, but also upregulated mRNA and protein abundance of sterol regulatory element-binding transcription factor 1 (SREBF1) and downregulated SIRT1 and peroxisome proliferator-activated receptor-gamma coactivator 1 alpha (PPARGC1A). Thus, these in vitro conditions were deemed optimal for subsequent experiments. Calf hepatocytes were cultured and incubated with OA (0.25 mM) for 24 h, followed by adenoviral AD-GFP-SIRT1-E or AD-GFP-SIRT1-N transduction for 48 h. Overexpression of SIRT1 led to greater protein and mRNA abundance of SIRT1 along with fatty acid oxidation-related genes including PPARGC1A, peroxisome proliferator-activated receptor alpha (PPARA), retinoid X receptor \u03b1 (RXRA), and ratio of phospho-acetyl-CoA carboxylase alpha (p-ACACA)\/total acetyl-CoA carboxylase alpha (ACACA). In contrast, it resulted in lower protein and mRNA abundance of genes related to lipid synthesis including SREBF1, fatty acid synthase (FASN), apolipoprotein E (APOE), and low-density lipoprotein receptor (LDLR). The concentration of TAG decreased due to SIRT1 overexpression. In contrast, silencing SIRT1 led to lower protein and mRNA abundance of SIRT1, PPARGC1A, PPARA, RXRA, and greater protein and mRNA abundance of SREBF1, FASN, APOE, and LDLR. Further, those responses were accompanied by greater content of cellular TAG and total cholesterol (TC). Overall, data from these in vitro studies indicated that SIRT1 is involved in the regulation of lipid metabolism in calf hepatocytes subjected to an increase in the supply of OA. Thus, it is possible that alterations in SIRT1 abundance and activity in vivo contribute to development of fatty liver in dairy cows.<\/jats:p>","DOI":"10.1093\/jas\/skab250","type":"journal-article","created":{"date-parts":[[2021,8,26]],"date-time":"2021-08-26T15:52:54Z","timestamp":1629993174000},"source":"Crossref","is-referenced-by-count":5,"title":["Sirtuin 1 is involved in oleic acid-induced calf hepatocyte steatosis via alterations in lipid metabolism-related proteins"],"prefix":"10.1093","volume":"99","author":[{"given":"Hongyan","family":"Ding","sequence":"first","affiliation":[{"name":"College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, Anhui, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu","family":"Li","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, Anhui, China"},{"name":"Anhui Province Key Laboratory of Veterinary Pathobiology and Disease Control, Hefei 230036, Anhui, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leihong","family":"Liu","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, Anhui, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ning","family":"Hao","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, Anhui, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Suping","family":"Zou","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, Anhui, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9522-4856","authenticated-orcid":false,"given":"Qianming","family":"Jiang","sequence":"additional","affiliation":[{"name":"Department of Animal Sciences and Division of Nutritional Sciences, University of Illinois, Urbana, IL 61801, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yusheng","family":"Liang","sequence":"additional","affiliation":[{"name":"Department of Animal Sciences and Division of Nutritional Sciences, University of Illinois, Urbana, IL 61801, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nana","family":"Ma","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, Jiangsu, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shibing","family":"Feng","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, Anhui, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xichun","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, Anhui, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9706-0808","authenticated-orcid":false,"given":"Jinjie","family":"Wu","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, Anhui, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1586-4365","authenticated-orcid":false,"given":"Juan J","family":"Loor","sequence":"additional","affiliation":[{"name":"Department of Animal Sciences and Division of Nutritional Sciences, University of Illinois, Urbana, IL 61801, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2021,8,26]]},"reference":[{"key":"2021100515440947900_CIT0001","doi-asserted-by":"crossref","first-page":"G833","DOI":"10.1152\/ajpgi.90358.2008","article-title":"Resveratrol alleviates alcoholic fatty liver in mice","volume":"295","author":"Ajmo","year":"2008","journal-title":"Am. J. Physiol. Gastrointest. Liver Physiol"},{"key":"2021100515440947900_CIT0052","journal-title":"J. Dairy Sci"},{"key":"2021100515440947900_CIT0002","doi-asserted-by":"crossref","first-page":"2804","DOI":"10.2527\/1995.7392804x","article-title":"Regulation of organic nutrient metabolism during transition from late pregnancy to early lactation","volume":"73","author":"Bell","year":"1995","journal-title":"J. Anim. Sci"},{"key":"2021100515440947900_CIT0003","doi-asserted-by":"crossref","first-page":"3105","DOI":"10.3168\/jds.S0022-0302(04)73446-3","article-title":"Invited review: pathology, etiology, prevention, and treatment of fatty liver in dairy cows","volume":"87","author":"Bobe","year":"2004","journal-title":"J. Dairy Sci"},{"key":"2021100515440947900_CIT0004","doi-asserted-by":"crossref","first-page":"838","DOI":"10.1016\/j.cmet.2012.04.022","article-title":"The NAD(+) precursor nicotinamide riboside enhances oxidative metabolism and protects against high-fat diet-induced obesity","volume":"15","author":"Cant\u00f3","year":"2012","journal-title":"Cell Metab"},{"key":"2021100515440947900_CIT0005","doi-asserted-by":"crossref","first-page":"1569","DOI":"10.5713\/ajas.2012.12682","article-title":"Effect of vitamin E and zinc supplementation on energy metabolites, lipid peroxidation, and milk production in peripartum sahiwal cows","volume":"26","author":"Chandra","year":"2013","journal-title":"Asian-Australas. J. Anim. Sci"},{"key":"2021100515440947900_CIT0006","doi-asserted-by":"crossref","first-page":"917","DOI":"10.1080\/13543784.2019.1657825","article-title":"Acetyl-CoA carboxylase (ACC) as a therapeutic target for metabolic syndrome and recent developments in ACC1\/2 inhibitors","volume":"28","author":"Chen","year":"2019","journal-title":"Expert Opin. Investig. Drugs"},{"key":"2021100515440947900_CIT0007","doi-asserted-by":"crossref","first-page":"1070","DOI":"10.1002\/jcb.25062","article-title":"BHBA influences bovine hepatic lipid metabolism via AMPK signaling pathway","volume":"116","author":"Deng","year":"2015","journal-title":"J. Cell. Biochem"},{"key":"2021100515440947900_CIT0008","doi-asserted-by":"crossref","first-page":"5561","DOI":"10.3168\/jds.2019-17652","article-title":"Mitochondrial membrane protein mitofusin 2 as a potential therapeutic target for treating free fatty acid-induced hepatic inflammation in dairy cows during early lactation","volume":"103","author":"Dong","year":"2020","journal-title":"J. Dairy Sci"},{"key":"2021100515440947900_CIT0009","doi-asserted-by":"crossref","first-page":"7536","DOI":"10.3168\/jds.2019-16544","article-title":"Low abundance of mitofusin 2 in dairy cows with moderate fatty liver is associated with alterations in hepatic lipid metabolism","volume":"102","author":"Dong","year":"2019","journal-title":"J. Dairy Sci"},{"key":"2021100515440947900_CIT0010","doi-asserted-by":"crossref","first-page":"1005","DOI":"10.1159\/000341477","article-title":"Increase of fatty acid oxidation and VLDL assembly and secretion overexpression of PTEN in cultured hepatocytes of newborn calf","volume":"30","author":"Fu","year":"2012","journal-title":"Cell. Physiol. Biochem"},{"key":"2021100515440947900_CIT0011","doi-asserted-by":"crossref","first-page":"1260","DOI":"10.3168\/jds.S0022-0302(97)76055-7","article-title":"Physiological changes at parturition and their relationship to metabolic disorders","volume":"80","author":"Goff","year":"1997","journal-title":"J. Dairy Sci"},{"key":"2021100515440947900_CIT0012","doi-asserted-by":"crossref","first-page":"3882","DOI":"10.3168\/jds.S0022-0302(93)77729-2","article-title":"Etiology of lipid-related metabolic disorders in periparturient dairy cows","volume":"76","author":"Grummer","year":"1993","journal-title":"J. Dairy Sci"},{"key":"2021100515440947900_CIT0013","first-page":"4279","article-title":"Targeting Sirt1 in a rat model of high-fat diet-induced non-alcoholic fatty liver disease: comparison of Gegen Qinlian decoction and resveratrol","volume":"14","author":"Guo","year":"2017","journal-title":"Exp. Ther. Med"},{"key":"2021100515440947900_CIT0053","journal-title":"J. Biol. Chem"},{"key":"2021100515440947900_CIT0014","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1038\/nrm3293","article-title":"Sirtuins as regulators of metabolism and healthspan","volume":"13","author":"Houtkooper","year":"2012","journal-title":"Nat. Rev. Mol. Cell Biol"},{"key":"2021100515440947900_CIT0015","doi-asserted-by":"crossref","first-page":"3199","DOI":"10.3168\/jds.2015-10198","article-title":"Stress, immunity, and the management of calves","volume":"99","author":"Hulbert","year":"2016","journal-title":"J. Dairy Sci"},{"key":"2021100515440947900_CIT0016","doi-asserted-by":"crossref","first-page":"833","DOI":"10.3168\/jds.2018-15208","article-title":"Perilipin 5 promotes hepatic steatosis in dairy cows through increasing lipid synthesis and decreasing very low density lipoprotein assembly","volume":"102","author":"Jia","year":"2018","journal-title":"J. Dairy Sci"},{"key":"2021100515440947900_CIT0017","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1016\/S0301-6226(00)00208-6","article-title":"Prevalence and indicators of post partum fatty infiltration of the liver in nine commercial dairy herds in the Netherlands","volume":"68","author":"Jorritsma","year":"2001","journal-title":"Livest. Prod. Sci"},{"key":"2021100515440947900_CIT0018","doi-asserted-by":"crossref","first-page":"1065","DOI":"10.1016\/S0093-691X(00)00415-5","article-title":"Relationships between fatty liver and fertility and some periparturient diseases in commercial Dutch dairy herds","volume":"54","author":"Jorritsma","year":"2000","journal-title":"Theriogenology"},{"key":"2021100515440947900_CIT0019","doi-asserted-by":"crossref","first-page":"835","DOI":"10.1111\/j.1939-1676.2007.tb03029.x","article-title":"Clinicopathologic evaluation of hepatic lipidosis in periparturient dairy cattle","volume":"21","author":"Kalaitzakis","year":"2007","journal-title":"J. Vet. Intern. Med"},{"key":"2021100515440947900_CIT0020","doi-asserted-by":"crossref","first-page":"385","DOI":"10.1016\/B978-0-12-407766-9.00016-X","article-title":"Sirtuin 1 deacetylase: a key regulator of hepatic lipid metabolism","volume":"91","author":"Kemper","year":"2013","journal-title":"Vitam. Horm"},{"key":"2021100515440947900_CIT0021","doi-asserted-by":"crossref","first-page":"418","DOI":"10.2337\/db14-0325","article-title":"ATGL-catalyzed lipolysis regulates SIRT1 to control PGC-1\u03b1\/PPAR-\u03b1 signaling","volume":"64","author":"Khan","year":"2015","journal-title":"Diabetes"},{"key":"2021100515440947900_CIT0022","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1515\/jbcpp-2017-0122","article-title":"Silymarin prevents lipid accumulation in the liver of rats with type 2 diabetes via sirtuin1 and SREBP-1c","volume":"29","author":"Kheiripour","year":"2018","journal-title":"J. Basic Clin. Physiol. Pharmacol"},{"key":"2021100515440947900_CIT0023","doi-asserted-by":"crossref","first-page":"1715","DOI":"10.3892\/ijmm.2016.2799","article-title":"Tangshen formula attenuates hepatic steatosis by inhibiting hepatic lipogenesis and augmenting fatty acid oxidation in db\/db mice","volume":"38","author":"Kong","year":"2016","journal-title":"Int J. Mol. Med."},{"key":"2021100515440947900_CIT0024","doi-asserted-by":"crossref","first-page":"27628","DOI":"10.1074\/jbc.M805711200","article-title":"SIRT1 modulation of the acetylation status, cytosolic localization, and activity of LKB1 possible role in AMP-activated protein kinase activation","volume":"283","author":"Lan","year":"2008","journal-title":"J. Biol. Chem"},{"key":"2021100515440947900_CIT0025","doi-asserted-by":"crossref","first-page":"445","DOI":"10.1016\/j.jsbmb.2013.08.013","article-title":"Acetic acid activates the AMP-activated protein kinase signaling pathway to regulate lipid metabolism in bovine hepatocytes","volume":"138","author":"Li","year":"2013","journal-title":"J. Steroid Biochem. Mol. Biol"},{"key":"2021100515440947900_CIT0026","doi-asserted-by":"crossref","first-page":"245","DOI":"10.3389\/fcell.2020.00245","article-title":"Non-esterified fatty acid induce dairy cow hepatocytes apoptosis via the mitochondria-mediated ROS-JNK\/ERK signaling pathway","volume":"8","author":"Li","year":"2020","journal-title":"Front. Cell Dev. Biol"},{"key":"2021100515440947900_CIT0027","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1017\/S0022029915000680","article-title":"High levels of acetoacetate and glucose increase expression of cytokines in bovine hepatocytes, through activation of the NF-\u03baB signalling pathway","volume":"83","author":"Li","year":"2016","journal-title":"J. Dairy Res"},{"key":"2021100515440947900_CIT0028","doi-asserted-by":"crossref","first-page":"202","DOI":"10.1016\/j.jff.2018.04.058","article-title":"Chitobiose alleviates oleic acid-induced lipid accumulation by decreasing fatty acid uptake and triglyceride synthesis in HepG2 cells","volume":"46","author":"Li","year":"2018","journal-title":"J. Funct. Foods"},{"key":"2021100515440947900_CIT0029","doi-asserted-by":"crossref","first-page":"560","DOI":"10.3390\/ani10040560","article-title":"Low expression of sirtuin 1 in the dairy cows with mild fatty liver alters hepatic lipid metabolism","volume":"10","author":"Li","year":"2020","journal-title":"Animals"},{"key":"2021100515440947900_CIT0030","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1007\/s11010-017-3149-z","article-title":"Resveratrol induces apoptosis and inhibits adipogenesis by stimulating the SIRT1-AMPKa-FOXO1 signalling pathway in bovine intramuscular adipocytes","volume":"439","author":"Liu","year":"2017","journal-title":"Mol. Cell Biochem"},{"key":"2021100515440947900_CIT0031","doi-asserted-by":"crossref","first-page":"485","DOI":"10.3390\/md17090485","article-title":"Inhibition effect of triglyceride accumulation by large yellow croaker roe DHA-PC in hepG2 cells","volume":"17","author":"Lu","year":"2019","journal-title":"Mar. Drugs"},{"key":"2021100515440947900_CIT0032","doi-asserted-by":"crossref","first-page":"711","DOI":"10.1007\/s12020-014-0465-x","article-title":"Plasma levels of SIRT1 associate with non-alcoholic fatty liver disease in obese patients","volume":"49","author":"Mariani","year":"2015","journal-title":"Endocrine"},{"key":"2021100515440947900_CIT0033","doi-asserted-by":"crossref","first-page":"5090","DOI":"10.3168\/jds.2011-4304","article-title":"Effects of encapsulated niacin on metabolism and production of periparturient dairy cows","volume":"94","author":"Morey","year":"2011","journal-title":"J. Dairy Sci"},{"key":"2021100515440947900_CIT0034","doi-asserted-by":"crossref","first-page":"224","DOI":"10.1186\/s12917-014-0224-5","article-title":"Effect of crude degummed canola oil and ad libitum grazing on plasma metabolites of primiparous Holstein-Friesian cows in a pasture-based system","volume":"10","author":"Otto","year":"2014","journal-title":"BMC Vet. Res"},{"key":"2021100515440947900_CIT0035","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1111\/j.1532-950X.1988.tb00283.x","article-title":"Partial hepatic resection for treatment of a single liver abscess in a dairy heifer","volume":"17","author":"Parker","year":"1988","journal-title":"Vet. Surg"},{"key":"2021100515440947900_CIT0036","doi-asserted-by":"crossref","first-page":"9793","DOI":"10.1073\/pnas.0802917105","article-title":"Sirt1 protects against high-fat diet-induced metabolic damage","volume":"105","author":"Paul","year":"2008","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2021100515440947900_CIT0037","doi-asserted-by":"crossref","first-page":"e45","DOI":"10.1093\/nar\/29.9.e45","article-title":"A new mathematical model for relative quantification in real-time RT-PCR","volume":"29","author":"Pfaffl","year":"2001","journal-title":"Nucleic Acids Res"},{"key":"2021100515440947900_CIT0038","doi-asserted-by":"crossref","first-page":"33959","DOI":"10.1074\/jbc.M110.122978","article-title":"SIRT1 deacetylates and inhibits SREBP-1C activity in regulation of hepatic lipid metabolism","volume":"285","author":"Ponugoti","year":"2010","journal-title":"J. Biol. Chem"},{"key":"2021100515440947900_CIT0039","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1016\/j.cmet.2009.02.006","article-title":"Hepatocyte-specific deletion of SIRT1 alters fatty acid metabolism and results in hepatic steatosis and inflammation","volume":"9","author":"Purushotham","year":"2009","journal-title":"Cell Metab"},{"key":"2021100515440947900_CIT0040","doi-asserted-by":"crossref","first-page":"601","DOI":"10.1016\/j.jhep.2016.11.004","article-title":"Aging aggravates alcoholic liver injury and fibrosis in mice by downregulating sirtuin 1 expression","volume":"66","author":"Ramirez","year":"2017","journal-title":"J. Hepatol"},{"key":"2021100515440947900_CIT0041","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1016\/j.febslet.2007.11.034","article-title":"Metabolic adaptations through the PGC-1 alpha and SIRT1 pathways","volume":"582","author":"Rodgers","year":"2008","journal-title":"FEBS Lett"},{"key":"2021100515440947900_CIT0042","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1038\/nature03354","article-title":"Nutrient control of glucose homeostasis through a complex of PGC-1alpha and SIRT1","volume":"434","author":"Rodgers","year":"2005","journal-title":"Nature"},{"key":"2021100515440947900_CIT0043","doi-asserted-by":"crossref","first-page":"12861","DOI":"10.1073\/pnas.0702509104","article-title":"Fasting-dependent glucose and lipid metabolic response through hepatic sirtuin 1","volume":"104","author":"Rodgers","year":"2007","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2021100515440947900_CIT0044","doi-asserted-by":"crossref","first-page":"1682","DOI":"10.3168\/jds.2018-15439","article-title":"High expression of cell death-inducing DFFA-like effector a (CIDEA) promotes milk fat content in dairy cows with clinical ketosis","volume":"102","author":"Sun","year":"2019","journal-title":"J. Dairy Sci"},{"key":"2021100515440947900_CIT0045","doi-asserted-by":"crossref","first-page":"12613","DOI":"10.1038\/srep12613","article-title":"Sirt1-deficiency causes defective protein quality control","volume":"5","author":"Tomita","year":"2015","journal-title":"Sci. Rep"},{"key":"2021100515440947900_CIT0047","doi-asserted-by":"crossref","first-page":"1207","DOI":"10.1016\/j.jnutbio.2014.06.001","article-title":"Alpha-lipoic acid improves high-fat diet-induced hepatic steatosis by modulating the transcription factors SREBP-1, FoxO1 and Nrf2 via the SIRT1\/LKB1\/AMPK pathway","volume":"25","author":"Yang","year":"2014","journal-title":"J. Nutr. Biochem"},{"key":"2021100515440947900_CIT0048","doi-asserted-by":"crossref","first-page":"801","DOI":"10.1053\/j.gastro.2013.11.008","article-title":"Deletion of SIRT1 from hepatocytes in mice disrupts lipin-1 signaling and aggravates alcoholic fatty liver","volume":"146","author":"Yin","year":"2014","journal-title":"Gastroenterology"},{"key":"2021100515440947900_CIT0049","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1016\/j.tvjl.2011.01.008","article-title":"An updated method for the isolation and culture of primary calf hepatocytes","volume":"191","author":"Zhang","year":"2012","journal-title":"Vet. J"},{"key":"2021100515440947900_CIT0050","doi-asserted-by":"crossref","first-page":"11945","DOI":"10.3168\/jds.2020-18684","article-title":"Mitochondrial dysfunction and endoplasmic reticulum stress in calf hepatocytes are associated with fatty acid-induced ORAI calcium release-activated calcium modulator 1 signaling","volume":"103","author":"Zhang","year":"2020","journal-title":"J. Dairy Sci"},{"key":"2021100515440947900_CIT0051","doi-asserted-by":"crossref","first-page":"303","DOI":"10.3892\/etm.2015.2858","article-title":"Effects of rhein lysinate on D-galactose-induced aging mice","volume":"11","author":"Zhen","year":"2016","journal-title":"Exp. Ther. Med"}],"container-title":["Journal of Animal Science"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/academic.oup.com\/jas\/advance-article-pdf\/doi\/10.1093\/jas\/skab250\/39978691\/skab250.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"http:\/\/academic.oup.com\/jas\/article-pdf\/99\/10\/skab250\/40509968\/skab250.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"http:\/\/academic.oup.com\/jas\/article-pdf\/99\/10\/skab250\/40509968\/skab250.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,10,8]],"date-time":"2021-10-08T05:10:56Z","timestamp":1633669856000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jas\/article\/doi\/10.1093\/jas\/skab250\/6358199"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,8,26]]},"references-count":52,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2021,8,26]]},"published-print":{"date-parts":[[2021,10,1]]}},"URL":"https:\/\/doi.org\/10.1093\/jas\/skab250","relation":{},"ISSN":["0021-8812","1525-3163"],"issn-type":[{"value":"0021-8812","type":"print"},{"value":"1525-3163","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2021,10,1]]},"published":{"date-parts":[[2021,8,26]]},"article-number":"skab250"}}