{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,26]],"date-time":"2025-11-26T05:04:52Z","timestamp":1764133492441,"version":"3.41.2"},"reference-count":44,"publisher":"Oxford University Press (OUP)","issue":"10","license":[{"start":{"date-parts":[[2021,10,7]],"date-time":"2021-10-07T00:00:00Z","timestamp":1633564800000},"content-version":"vor","delay-in-days":6,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"name":"Diamond V Mills, Inc"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,10,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Dietary intervention may be a valuable strategy to optimize the intra-articular environment in young horses to prolong their performance career. To test the hypothesis that dietary supplementation of a Saccharomyces cerevisiae fermentation product would reduce markers of joint inflammation and increase markers of cartilage metabolism following a single inflammatory insult, Quarter Horse yearlings (mean \u00b1 SD; 9 \u00b1 1.0 mo) were balanced by age, sex, body weight (BW), and farm of origin and randomly assigned to the following treatment groups: 1.25% BW\/d (dry matter basis) custom-formulated concentrate only (CON; n = 9) or concentrate top-dressed with 21 g\/d S. cerevisiae fermentation product (SCFP; n = 10) for 98 d. Horses had ad libitum access to Coastal bermudagrass hay. On day 84, one randomly selected radial carpal joint from each horse was injected with 0.5 ng lipopolysaccharide (LPS) solution. The remaining carpal joint was injected with sterile lactated Ringer\u2019s solution as a contralateral control. Synovial fluid obtained before supplementation (day 0) and on day 84 at preinjection hour 0 and 6, 12, 24, 168, and 336 h postinjection was analyzed for prostaglandin E2 (PGE2), carboxypropeptide of type II collagen (CPII), and collagenase cleavage neopeptide (C2C) by commercial assays. Rectal temperature, heart rate, respiration rate, carpal surface temperature, and carpal circumference were recorded prior to each sample collection and for 24 h postinjection. Data were analyzed using linear models with repeated measures. From day 0 to 84, synovial C2C declined (P \u2264 0.01) and the CPII:C2C ratio increased (P \u2264 0.01) in all horses with no effect of diet. In response to intra-articular LPS, synovial PGE2 increased by hour 6 (P \u2264 0.01) and returned to baseline by hour 336; CPII increased by hour 12, remained elevated through hour 168 (P \u2264 0.01), and returned to baseline by hour 336; and C2C increased by hour 6 (P \u2264 0.01) but did not return to baseline through hour 336 (P \u2264 0.01). Post-intra-articular injection, PGE2 levels were lower in SCFP than CON horses (P = 0.01) regardless of injection type. Synovial CPII and the CPII:C2C ratio demonstrated stability during the LPS challenge in SCFP compared with CON horses (P \u2264 0.01). Clinical parameters were not influenced by diet but increased in response to repeated arthrocentesis (P \u2264 0.01). Dietary SCFP may favorably modulate intra-articular inflammation following an acute stressor and influence cartilage turnover in young horses.<\/jats:p>","DOI":"10.1093\/jas\/skab272","type":"journal-article","created":{"date-parts":[[2021,10,8]],"date-time":"2021-10-08T00:13:15Z","timestamp":1633651995000},"source":"Crossref","is-referenced-by-count":6,"title":["Responses to an intra-articular lipopolysaccharide challenge following dietary supplementation of<i>Saccharomyces cerevisiae<\/i>fermentation product in young horses"],"prefix":"10.1093","volume":"99","author":[{"given":"Rafael E","family":"Martinez","sequence":"first","affiliation":[{"name":"Department of Animal Science, Texas A&M University and Texas A&M AgriLife Research, College Station, TX 77843, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jessica L","family":"Leatherwood","sequence":"additional","affiliation":[{"name":"Department of Animal Science, Texas A&M University and Texas A&M AgriLife Research, College Station, TX 77843, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carolyn E","family":"Arnold","sequence":"additional","affiliation":[{"name":"Department of Large Animal Clinical Sciences, Texas A&M University, College Station, TX 77843, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kati G","family":"Glass","sequence":"additional","affiliation":[{"name":"Department of Large Animal Clinical Sciences, Texas A&M University, College Station, TX 77843, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kelly W","family":"Walter","sequence":"additional","affiliation":[{"name":"Department of Agricultural Science, Truman State University, Kirksville, MO 63501, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hannah C","family":"Valigura","sequence":"additional","affiliation":[{"name":"Department of Animal Science, Texas A&M University and Texas A&M AgriLife Research, College Station, TX 77843, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sharon A","family":"Norton","sequence":"additional","affiliation":[{"name":"Diamond V Mills, Inc., Cedar Rapids, IA 52404, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sarah H","family":"White-Springer","sequence":"additional","affiliation":[{"name":"Department of Animal Science, Texas A&M University and Texas A&M AgriLife Research, College Station, TX 77843, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2021,10,7]]},"reference":[{"key":"2021103113294009000_CIT0001","doi-asserted-by":"crossref","first-page":"1204","DOI":"10.3389\/fmicb.2016.01204","article-title":"Gut Bifidobacteria populations in human health and aging","volume":"7","author":"Arboleya","year":"2016","journal-title":"Front. 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