{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T11:54:43Z","timestamp":1753876483807,"version":"3.41.2"},"reference-count":68,"publisher":"Oxford University Press (OUP)","issue":"10","license":[{"start":{"date-parts":[[2021,10,6]],"date-time":"2021-10-06T00:00:00Z","timestamp":1633478400000},"content-version":"vor","delay-in-days":5,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/100005825","name":"National Institute of Food and Agriculture","doi-asserted-by":"publisher","award":["IDA01570"],"award-info":[{"award-number":["IDA01570"]}],"id":[{"id":"10.13039\/100005825","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,10,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Because of its high crude protein (CP) content, dietary inclusion of corn dried distillers\u2019 grains with solubles (DDGS) in finishing cattle diets can increase the ruminal loss of ammonia-nitrogen (NH3-N), which ends up excreted as urine urea-N (UUN). Increasing dietary fermentable energy supply can enhance ruminal use of N; however, it could also lead to acidotic conditions that compromise digestive function and animal performance. We evaluated the effects of partially replacing dietary corn grain with 20% or 40% (dry matter [DM] basis) wheat grain in finishing diets containing 15% corn DDGS on N utilization, ruminal pH, and digestive function. Nutrient intake and digestion, ruminal fermentation characteristics, microbial protein synthesis, route of N excretion, and blood metabolites were measured. Six ruminally fistulated crossbred beef heifers (initial body weight \u00b1 SD; 797 \u00b1 58.8 kg) were used in a replicated 3 \u00d7 3 Latin square design with 28-d periods. Dietary treatments were either corn (73% of diet DM; CON), 53:20 corn:wheat blend (20W), or 33:40 corn:wheat blend (40W) as the major fermentable energy source. Dry matter intake (DMI) tended to be lower for heifers fed the 40W than CON and 20W diets. Feeding diets containing wheat grain led to an increase (P = 0.04) in neutral detergent fiber (NDF) intake. However, there was no diet effect (P \u2265 0.60) on apparent total tract DM and NDF digestibility. Feeding wheat grain led to a decrease (P \u2264 0.03) in mean and minimum pH, an increase (P = 0.04) in pH &amp;lt; 5.8 duration, and a tendency for an increase in the area and acidosis index for pH &amp;lt; 5.8 and 5.5. Nitrogen intake, which was lower (P = 0.04) for 40W than 20W heifers did not differ between CON and 20W heifers. There was no diet effect (P = 0.80) on ruminal NH3-N concentration and estimated microbial N flow. However, feeding diets containing wheat grain led to a decrease (P = 0.045) in UUN excretion (% total urine N). Fecal and total N excretion (% of N intake) increased (P &amp;lt; 0.01) following the addition of wheat grain to the diet. Apparent N retention was lower (P = 0.03) for 40W than CON and 20W heifers. In summary, although it led to a desirable decrease in UUN excretion, feeding wheat grain in corn DDGS-containing diets increased acidotic conditions in the rumen, which possibly led to the tendency for a decrease in DMI. The negative apparent N retention at the 40% wheat grain inclusion also suggests a decrease in nutrient supply, which could compromise feedlot performance.<\/jats:p>","DOI":"10.1093\/jas\/skab281","type":"journal-article","created":{"date-parts":[[2021,10,6]],"date-time":"2021-10-06T20:25:53Z","timestamp":1633551953000},"source":"Crossref","is-referenced-by-count":2,"title":["Increasing dietary proportion of wheat grain in finishing diets containing distillers\u2019 grains: impact on nitrogen utilization, ruminal pH, and digestive function"],"prefix":"10.1093","volume":"99","author":[{"given":"Cody N","family":"Ream","sequence":"first","affiliation":[{"name":"Department of Animal, Veterinary and Food Sciences, University of Idaho , Moscow, ID 83843 , USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9207-583X","authenticated-orcid":false,"given":"Gwinyai E","family":"Chibisa","sequence":"additional","affiliation":[{"name":"Department of Animal, Veterinary and Food Sciences, University of Idaho , Moscow, ID 83843 , USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2021,10,6]]},"reference":[{"key":"2022100600084215000_CIT0001","doi-asserted-by":"crossref","first-page":"2714","DOI":"10.3168\/jds.2018-14721","article-title":"Effect of dietary fat supplementation on methane emissions from dairy cows fed wheat or corn","volume":"102","author":"Alvarez-Hess","year":"2019","journal-title":"J. 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