{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T23:40:17Z","timestamp":1790725217298,"version":"4.1.0"},"reference-count":49,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2022,2,1]],"date-time":"2022-02-01T00:00:00Z","timestamp":1643673600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,2,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Disruption of the ruminal epithelium barrier occurs during subacute ruminal acidosis due to low pH, hyper-osmolality, and increased concentrations of lipopolysaccharide and histamine in ruminal fluid. However, the individual roles of lipopolysaccharide and histamine in the process of ruminal epithelium barriers disruption are not clear. The objective of the present investigation was to evaluate the direct effect of lipopolysaccharide and histamine on the barrier function of the ruminal epithelium. Compared with control (CON), histamine (HIS, 20 \u03bcM) increased the short-circuit current (Isc; 88.2%, P &amp;lt; 0.01), transepithelial conductance (Gt; 29.7%, P = 0.056), and the permeability of fluorescein 5(6)-isothiocyanate (FITC) (1.04-fold, P &amp;lt; 0.01) of ruminal epithelium. The apparent permeability of LPS was 1.81-fold higher than HIS (P &amp;lt; 0.01). The mRNA abundance of OCLN in ruminal epithelium was decreased by HIS (1.1-fold, P = 0.047). The results of the present study suggested that mucosal histamine plays a direct role in the disruption of ruminal epithelium barrier function, whereas lipopolysaccharide (at a pH of 7.4) has no effect on the permeability of rumen tissues ex vivo.<\/jats:p>","DOI":"10.1093\/jas\/skac005","type":"journal-article","created":{"date-parts":[[2022,2,27]],"date-time":"2022-02-27T03:49:33Z","timestamp":1645933773000},"source":"Crossref","is-referenced-by-count":14,"title":["Direct effect of lipopolysaccharide and histamine on permeability of the rumen epithelium of steers ex vivo"],"prefix":"10.1093","volume":"100","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9282-248X","authenticated-orcid":false,"given":"Shengtao","family":"Gao","sequence":"first","affiliation":[{"name":"State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 100193, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alateng","family":"Zhula","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 100193, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenhui","family":"Liu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 100193, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhongyan","family":"Lu","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zanming","family":"Shen","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6396-2130","authenticated-orcid":false,"given":"Gregory B","family":"Penner","sequence":"additional","affiliation":[{"name":"Department of Animal and Poultry Science, University of Saskatchewan, Saskatchewan, S7N 5A8, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lu","family":"Ma","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 100193, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dengpan","family":"Bu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 100193, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2022,2,27]]},"reference":[{"issue":"2","key":"2022022708492158400_CIT0001","doi-asserted-by":"crossref","first-page":"464","DOI":"10.2527\/2000.782464x","article-title":"Effect and absorption of histamine in sheep rumen: significance of acidotic epithelial damage.","volume":"78","author":"Aschenbach","year":"2000","journal-title":"J. Anim. Sci"},{"issue":"6-7","key":"2022022708492158400_CIT0002","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1111\/j.1439-0442.1998.tb00843.x","article-title":"Histamine affects growth of sheep ruminal epithelial cells kept in primary culture.","volume":"45","author":"Aschenbach","year":"1998","journal-title":"Zentralbl Veterinarmed A"},{"issue":"1","key":"2022022708492158400_CIT0003","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1007\/s004240000285","article-title":"Transport, catabolism and release of histamine in the ruminal epithelium of sheep.","volume":"440","author":"Aschenbach","year":"2000","journal-title":"Pfl\u00fcgers Archiv"},{"issue":"2","key":"2022022708492158400_CIT0004","doi-asserted-by":"crossref","first-page":"1866","DOI":"10.3168\/jds.2018-15243","article-title":"Symposium review: the importance of the ruminal epithelial barrier for a healthy and productive cow.","volume":"102","author":"Aschenbach","year":"2019","journal-title":"J. Dairy Sci"},{"issue":"4","key":"2022022708492158400_CIT0005","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1016\/S0966-842X(00)88906-4","article-title":"Bacterial translocation from the gastrointestinal tract.","volume":"3","author":"Berg","year":"1995","journal-title":"Trends Microbiol"},{"issue":"36","key":"2022022708492158400_CIT0006","doi-asserted-by":"crossref","first-page":"38578","DOI":"10.18632\/oncotarget.6161","article-title":"Hepatic TLR4 signaling is activated by LPS from digestive tract during SARA, and epigenetic mechanisms contribute to enforced TLR4 expression.","volume":"6","author":"Chang","year":"2015","journal-title":"Oncotarget"},{"issue":"9","key":"2022022708492158400_CIT0007","first-page":"1789","article-title":"Catabolism of orally administered histamine in sheep.","volume":"33","author":"Dickinson","year":"1972","journal-title":"Am. J. Vet. Res"},{"issue":"2","key":"2022022708492158400_CIT0008","doi-asserted-by":"crossref","first-page":"e0172674","DOI":"10.1371\/journal.pone.0172674","article-title":"Selection of internal reference genes for normalization of reverse transcription quantitative polymerase chain reaction (RT-qPCR) analysis in the rumen epithelium.","volume":"12","author":"Die","year":"2017","journal-title":"PLoS One"},{"issue":"12","key":"2022022708492158400_CIT0009","doi-asserted-by":"crossref","first-page":"e82850","DOI":"10.1371\/journal.pone.0082850","article-title":"Long-term effects of subacute ruminal acidosis (SARA) on milk quality and hepatic gene expression in lactating goats fed a high-concentrate diet.","volume":"8","author":"Dong","year":"2013","journal-title":"PLoS One"},{"issue":"2","key":"2022022708492158400_CIT0010","doi-asserted-by":"crossref","first-page":"138","DOI":"10.1046\/j.1365-2362.2001.00792.x","article-title":"Effect of ischemia on intestinal permeability of lipopolysaccharides.","volume":"31","author":"Drewe","year":"2001","journal-title":"Eur. J. Clin. Invest"},{"issue":"12","key":"2022022708492158400_CIT0011","doi-asserted-by":"crossref","first-page":"5552","DOI":"10.3168\/jds.2007-0257","article-title":"Acidosis and lipopolysaccharide from Escherichia coli B:055 cause hyperpermeability of rumen and colon tissues.","volume":"90","author":"Emmanuel","year":"2007","journal-title":"J. Dairy Sci"},{"issue":"2","key":"2022022708492158400_CIT0012","doi-asserted-by":"crossref","first-page":"606","DOI":"10.3168\/jds.2007-0256","article-title":"Feeding high proportions of barley grain stimulates an inflammatory response in dairy cows.","volume":"91","author":"Emmanuel","year":"2008","journal-title":"J. Dairy Sci"},{"issue":"6","key":"2022022708492158400_CIT0013","doi-asserted-by":"crossref","first-page":"5040","DOI":"10.3168\/jds.2016-11913","article-title":"The effects of heat stress on protein metabolism in lactating holstein cows.","volume":"100","author":"Gao","year":"2017","journal-title":"J. Dairy Sci"},{"issue":"1","key":"2022022708492158400_CIT0014","doi-asserted-by":"crossref","first-page":"R173","DOI":"10.1152\/ajpregu.00425.2004","article-title":"Functional organization of the bovine rumen epithelium","volume":"288","author":"Graham","year":"2005","journal-title":"Am. J. Physiol. Regul. Integr. Comp. Physiol"},{"issue":"1","key":"2022022708492158400_CIT0015","doi-asserted-by":"crossref","first-page":"126","DOI":"10.1093\/jas\/skx017","article-title":"Effect of individual SCFA on the epithelial barrier of sheep rumen under physiological and acidotic luminal pH conditions.","volume":"96","author":"Greco","year":"2018","journal-title":"J. Anim. Sci"},{"issue":"29","key":"2022022708492158400_CIT0016","doi-asserted-by":"crossref","first-page":"46769","DOI":"10.18632\/oncotarget.18151","article-title":"Rumen-derived lipopolysaccharide provoked inflammatory injury in the liver of dairy cows fed a high-concentrate diet.","volume":"8","author":"Guo","year":"2017","journal-title":"Oncotarget"},{"key":"2022022708492158400_CIT0017","doi-asserted-by":"crossref","first-page":"702572","DOI":"10.1155\/2014\/702572","article-title":"Ruminal acidosis in feedlot: from aetiology to prevention.","volume":"2014","author":"Hernandez","year":"2014","journal-title":"Sci. World J"},{"issue":"2","key":"2022022708492158400_CIT0018","doi-asserted-by":"crossref","first-page":"380","DOI":"10.1111\/jpn.12850","article-title":"Signals for identifying cows at risk of subacute ruminal acidosis in dairy veterinary practice.","volume":"102","author":"Humer","year":"2018","journal-title":"J. Anim. Physiol. Anim. Nutr. (Berl)"},{"issue":"1","key":"2022022708492158400_CIT0019","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1113\/jphysiol.1974.sp010743","article-title":"Absorption and catabolism of histamine in sheep","volume":"243","author":"Kay","year":"1974","journal-title":"J. Physiol"},{"issue":"10","key":"2022022708492158400_CIT0020","doi-asserted-by":"crossref","first-page":"9587","DOI":"10.3168\/jds.2020-18652","article-title":"Effects of lipopolysaccharide exposure in primary bovine ruminal epithelial cells.","volume":"103","author":"Kent-Dennis","year":"2020","journal-title":"J. Dairy Sci"},{"issue":"3","key":"2022022708492158400_CIT0021","doi-asserted-by":"crossref","first-page":"1060","DOI":"10.3168\/jds.2008-1389","article-title":"A grain-based subacute ruminal acidosis challenge causes translocation of lipopolysaccharide and triggers inflammation.","volume":"92","author":"Khafipour","year":"2009","journal-title":"J. Dairy Sci"},{"issue":"7","key":"2022022708492158400_CIT0022","doi-asserted-by":"crossref","first-page":"517","DOI":"10.1136\/gut.17.7.517","article-title":"Permeability of the small intestine after intra-arterial injection of histamine-type mediators and irradiation","volume":"17","author":"Kingham","year":"1976","journal-title":"Gut"},{"issue":"4","key":"2022022708492158400_CIT0023","doi-asserted-by":"crossref","first-page":"2293","DOI":"10.3168\/jds.2012-6187","article-title":"Feeding barley grain-rich diets altered electrophysiological properties and permeability of the ruminal wall in a goat model","volume":"96","author":"Klevenhusen","year":"2013","journal-title":"J. Dairy Sci"},{"issue":"3","key":"2022022708492158400_CIT0024","doi-asserted-by":"crossref","first-page":"R232","DOI":"10.1152\/ajpregu.00068.2013","article-title":"A high-grain diet causes massive disruption of ruminal epithelial tight junctions in goats.","volume":"305","author":"Liu","year":"2013","journal-title":"Am. J. Physiol. Regul. Integr. Comp. Physiol"},{"issue":"5","key":"2022022708492158400_CIT0025","doi-asserted-by":"crossref","first-page":"1452","DOI":"10.2527\/jas.2011-4627","article-title":"Growth and development symposium: endotoxin, inflammation, and intestinal function in livestock.","volume":"90","author":"Mani","year":"2012","journal-title":"J. Anim. Sci"},{"issue":"1","key":"2022022708492158400_CIT0026","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1186\/1743-7075-10-6","article-title":"Dietary oil composition differentially modulates intestinal endotoxin transport and postprandial endotoxemia","volume":"10","author":"Mani","year":"2013","journal-title":"Nutr. Metab. (Lond)"},{"issue":"2","key":"2022022708492158400_CIT0027","doi-asserted-by":"crossref","first-page":"525","DOI":"10.1111\/1462-2920.12724","article-title":"Microbiome-metabolome analysis reveals unhealthy alterations in the composition and metabolism of ruminal microbiota with increasing dietary grain in a goat model","volume":"18","author":"Mao","year":"2016","journal-title":"Environ. Microbiol"},{"key":"2022022708492158400_CIT0028","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1146\/annurev.pathol.4.110807.092135","article-title":"Epithelial barriers in homeostasis and disease","volume":"5","author":"Marchiando","year":"2010","journal-title":"Annu. Rev. Pathol"},{"issue":"1","key":"2022022708492158400_CIT0029","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1002\/iub.1347","article-title":"Claudin clusters as determinants of epithelial barrier function","volume":"67","author":"Markov","year":"2015","journal-title":"IUBMB Life"},{"issue":"5","key":"2022022708492158400_CIT0030","doi-asserted-by":"crossref","first-page":"290","DOI":"10.1002\/iub.1622","article-title":"The epithelial barrier and beyond: claudins as amplifiers of physiological organ functions","volume":"69","author":"Markov","year":"2017","journal-title":"IUBMB Life"},{"issue":"8","key":"2022022708492158400_CIT0031","doi-asserted-by":"crossref","first-page":"6662","DOI":"10.3168\/jds.2016-12262","article-title":"Key role of short-chain fatty acids in epithelial barrier failure during ruminal acidosis","volume":"100","author":"Meissner","year":"2017","journal-title":"J. Dairy Sci"},{"issue":"5","key":"2022022708492158400_CIT0032","doi-asserted-by":"crossref","first-page":"1537","DOI":"10.1016\/0016-5085(92)91175-4","article-title":"Rabbit gut permeability in response to histamine chloramines and chemotactic peptide","volume":"103","author":"Miller","year":"1992","journal-title":"Gastroenterology"},{"issue":"3","key":"2022022708492158400_CIT0033","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1111\/j.1476-5381.1978.tb08465.x","article-title":"H1- and H2-receptors in the smooth muscle of the ruminant stomach","volume":"62","author":"Ohga","year":"1978","journal-title":"Br. J. Pharmacol"},{"issue":"1","key":"2022022708492158400_CIT0034","doi-asserted-by":"crossref","first-page":"275","DOI":"10.2527\/1998.761275x","article-title":"Acidosis in cattle: a review","volume":"76","author":"Owens","year":"1998","journal-title":"J. Anim. Sci"},{"issue":"10","key":"2022022708492158400_CIT0035","doi-asserted-by":"crossref","first-page":"4838","DOI":"10.3168\/jds.2010-3406","article-title":"A single mild episode of subacute ruminal acidosis does not affect ruminal barrier function in the short term.","volume":"93","author":"Penner","year":"2010","journal-title":"J. Dairy Sci"},{"issue":"1","key":"2022022708492158400_CIT0036","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1016\/j.livsci.2012.08.009","article-title":"Starch source in high concentrate rations does not affect rumen pH, histamine and lipopolysaccharide concentrations in dairy cows.","volume":"150","author":"Pilachai","year":"2012","journal-title":"Livest. Sci"},{"issue":"1","key":"2022022708492158400_CIT0037","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/j.tvjl.2007.12.016","article-title":"Subacute ruminal acidosis in dairy cows: the physiological causes, incidence and consequences","volume":"176","author":"Plaizier","year":"2008","journal-title":"Vet. J"},{"issue":"7","key":"2022022708492158400_CIT0038","doi-asserted-by":"crossref","first-page":"R802","DOI":"10.1152\/ajpregu.00035.2014","article-title":"Short-term adaptation of the ruminal epithelium involves abrupt changes in sodium and short-chain fatty acid transport","volume":"307","author":"Schurmann","year":"2014","journal-title":"Am. J. Physiol. Regul. Integr. Comp. Physiol"},{"issue":"3","key":"2022022708492158400_CIT0039","first-page":"319","article-title":"Potentiation by aminoguanidine of the sensitivity of sheep to histamine given by mouth. Effect of amino-guanidine on the urinary excretion of endogenous histamine","volume":"52","author":"Sjaastad","year":"1967","journal-title":"Q. J. Exp. Physiol. Cogn. Med. Sci"},{"issue":"2","key":"2022022708492158400_CIT0040","doi-asserted-by":"crossref","first-page":"1297","DOI":"10.3168\/jds.2017-12896","article-title":"Subacute ruminal acidosis affects fermentation and endotoxin concentration in the rumen and relative expression of the CD14\/TLR4\/MD2 genes involved in lipopolysaccharide systemic immune response in dairy cows","volume":"101","author":"Stefanska","year":"2018","journal-title":"J. Dairy Sci"},{"issue":"3","key":"2022022708492158400_CIT0041","doi-asserted-by":"crossref","first-page":"1109","DOI":"10.1159\/000478765","article-title":"Histamine induces bovine rumen epithelial cell inflammatory response via NF-kappaB pathway","volume":"42","author":"Sun","year":"2017","journal-title":"Cell. Physiol. Biochem"},{"issue":"1","key":"2022022708492158400_CIT0042","doi-asserted-by":"crossref","first-page":"e13357","DOI":"10.1111\/asj.13357","article-title":"Regulation of the intestinal barrier by nutrients: the role of tight junctions","volume":"91","author":"Suzuki","year":"2020","journal-title":"Anim. Sci. J"},{"issue":"1","key":"2022022708492158400_CIT0043","doi-asserted-by":"crossref","first-page":"33","DOI":"10.2133\/dmpk.19.33","article-title":"Lipopolysaccharide transport system across colonic epithelial cells in normal and infective rat","volume":"19","author":"Tomita","year":"2004","journal-title":"Drug Metab. Pharmacokinet"},{"issue":"4","key":"2022022708492158400_CIT0044","doi-asserted-by":"crossref","first-page":"4260","DOI":"10.3168\/jds.2020-18738","article-title":"Effects of subacute ruminal acidosis on colon epithelial morphological structure, permeability, and expression of key tight junction proteins in dairy goats","volume":"104","author":"Wang","year":"2021","journal-title":"J. Dairy Sci"},{"key":"2022022708492158400_CIT0045","doi-asserted-by":"crossref","first-page":"100370","DOI":"10.1016\/j.animal.2021.100370","article-title":"Thiamine modulates intestinal morphological structure and microbiota under subacute ruminal acidosis induced by a high-concentrate diet in Saanen goats.","volume":"15","author":"Wen","year":"2021","journal-title":"Animal"},{"key":"2022022708492158400_CIT0046","doi-asserted-by":"crossref","first-page":"3153","DOI":"10.2527\/jas.2011-4315","article-title":"Supplemental butyrate does not enhance the absorptive or barrier functions of the isolated ovine ruminal epithelia.","volume":"90","author":"Wilson","year":"2012","journal-title":"J. Anim. Sci"},{"issue":"3","key":"2022022708492158400_CIT0047","doi-asserted-by":"crossref","first-page":"1099","DOI":"10.1016\/j.rvsc.2012.02.004","article-title":"Interplay between rumen digestive disorders and diet-induced inflammation in dairy cattle.","volume":"93","author":"Zebeli","year":"2012","journal-title":"Res. Vet. Sci"},{"issue":"9","key":"2022022708492158400_CIT0048","doi-asserted-by":"crossref","first-page":"1886","DOI":"10.1002\/jsfa.6508","article-title":"Effect of dietary forage sources on rumen microbiota, rumen fermentation and biogenic amines in dairy cows.","volume":"94","author":"Zhang","year":"2014","journal-title":"J. Sci. Food Agric"},{"issue":"1","key":"2022022708492158400_CIT0049","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1186\/s12917-018-1463-7","article-title":"Inflammatory mechanism of rumenitis in dairy cows with subacute ruminal acidosis.","volume":"14","author":"Zhao","year":"2018","journal-title":"BMC Vet. Res"}],"container-title":["Journal of Animal Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/jas\/article-pdf\/100\/2\/skac005\/42621536\/skac005.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jas\/article-pdf\/100\/2\/skac005\/42621536\/skac005.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,27]],"date-time":"2022-02-27T03:49:57Z","timestamp":1645933797000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jas\/article\/doi\/10.1093\/jas\/skac005\/6537709"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,2,1]]},"references-count":49,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2022,2,27]]},"published-print":{"date-parts":[[2022,2,1]]}},"URL":"https:\/\/doi.org\/10.1093\/jas\/skac005","relation":{},"ISSN":["0021-8812","1525-3163"],"issn-type":[{"value":"0021-8812","type":"print"},{"value":"1525-3163","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2022,2,1]]},"published":{"date-parts":[[2022,2,1]]},"article-number":"skac005"}}