{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T11:21:07Z","timestamp":1790508067199,"version":"4.1.0"},"reference-count":46,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[2022,2,24]],"date-time":"2022-02-24T00:00:00Z","timestamp":1645660800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/100019191","name":"Texas A&M AgriLife Research","doi-asserted-by":"crossref","award":["H-8200"],"award-info":[{"award-number":["H-8200"]}],"id":[{"id":"10.13039\/100019191","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,4,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>This study determined the oxidation of amino acids, glucose and fatty acid in enterocytes of developing chickens. Jejunal enterocytes were isolated from 0-, 7-, 21-, and 42-d-old broiler chickens, and incubated at 40\u00b0C for 30\u00a0min in Krebs\u2013Henseleit bicarbonate buffer (pH 7.4) containing 5\u00a0mM D-glucose and one of the following: 0.5\u20135\u00a0mM L-[U-14C]glutamate, 0.5\u20135\u00a0mM L-[U-14C]glutamine, 0.5\u20135\u00a0mM L-[U-14C]aspartate, 0.5\u20135\u00a0mM L-[U-14C]alanine, 0.5\u20132\u00a0mM [U-14C]palmitate, D-[U-14C]glucose, 0.5\u20135\u00a0mM [U-14C]propionate, and 0.5\u20135\u00a0mM [1-14C]butyrate. 14CO2 produced from each 14C-labeled substrate was collected for determination of radioactivity. Among all the substrates studied, glutamate had the greatest rate of oxidation in enterocytes from 0- to 42-d-old chickens. Glutamate transaminases, rather than glutamate dehydrogenase, may be primarily responsible for initiating glutamate degradation. Rates of amino acid and fatty acid oxidation by cells increased (P &amp;lt; 0.05) with increasing their extracellular concentrations from 0.5 to 5\u00a0mM. Rates of glutamate and glucose oxidation in enterocytes decreased (P &amp;lt; 0.05) with increasing age, and rates of glutamine, aspartate, propionate, and butyrate oxidation were lower (P &amp;lt; 0.05) in 42-d-old chickens than in 0-d-old chickens. By contrast, oxidation of palmitate at 2\u00a0mM increased (P &amp;lt; 0.05) by 118% in cells from 42-d-old chickens, compared with 0-d-old chickens. Compared with glutamate, oxidation of glutamine, aspartate, alanine, propionate, butyrate, and palmitate was limited in cells from all age groups of chickens. Collectively, these results indicate that glutamate is the major metabolic fuel in enterocytes of 0- to 42-d-old chickens.<\/jats:p>","DOI":"10.1093\/jas\/skac053","type":"journal-article","created":{"date-parts":[[2022,2,24]],"date-time":"2022-02-24T08:20:25Z","timestamp":1645690825000},"source":"Crossref","is-referenced-by-count":26,"title":["Oxidation of amino acids, glucose, and fatty acids as metabolic fuels in enterocytes of post-hatching developing chickens"],"prefix":"10.1093","volume":"100","author":[{"given":"Wenliang","family":"He","sequence":"first","affiliation":[{"name":"Department of Animal Science, Texas A&M University, College Station, TX 77843, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kyohei","family":"Furukawa","sequence":"additional","affiliation":[{"name":"Department of Animal Science, Texas A&M University, College Station, TX 77843, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher A","family":"Bailey","sequence":"additional","affiliation":[{"name":"Department of Poultry Science, Texas A&M University, College Station, TX 77843, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8058-6969","authenticated-orcid":false,"given":"Guoyao","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Animal Science, Texas A&M University, College Station, TX 77843, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2022,2,24]]},"reference":[{"key":"2022041010174143400_CIT0001","doi-asserted-by":"crossref","first-page":"115","DOI":"10.5772\/intechopen.76972","article-title":"Managing dietary energy intake by broiler chickens to reduce production costs and improve product quality.","volume-title":"Animal husbandry and nutrition","author":"Ahiwe","year":"2018"},{"key":"2022041010174143400_CIT0002","doi-asserted-by":"crossref","first-page":"474","DOI":"10.1186\/2193-1801-3-474","article-title":"Rapid publication-ready MS-Word tables for one-way ANOVA.","volume":"3","author":"Assaad","year":"2014","journal-title":"Springerplus"},{"key":"2022041010174143400_CIT0003","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1016\/B978-0-12-817052-6.00021-5","article-title":"Precision poultry nutrition and feed formulation.","volume-title":"Animal agriculture: challenges, innovations, and sustainability","author":"Bailey","year":"2020"},{"key":"2022041010174143400_CIT0004","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/978-3-030-45328-2_1","article-title":"Amino acids in intestinal physiology and health.","volume":"1265","author":"Beaumont","year":"2020","journal-title":"Adv. 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