{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,14]],"date-time":"2026-03-14T15:26:29Z","timestamp":1773501989513,"version":"3.50.1"},"reference-count":54,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2022,3,1]],"date-time":"2022-03-01T00:00:00Z","timestamp":1646092800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,3,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Due to the large amounts of calcium transferred to milk from mammary glands, periparturient dairy goats face challenges with calcium metabolism disorder and hypocalcemia. Serotonin (5-hydroxytryptamine, 5-HT), the product of 5-hydroxy-l-tryptophan (5-HTP) catalyzed by tryptophan hydroxylase 1, is a multifunctional monoamine thought to be a homeostatic regulator of the animal. The objective of the current study was to investigate the effects and underlying mechanisms of intramuscular 5-HTP injections on calcium homeostasis in the goat mammary glands. In the in vivo experiment, 30 multiparous Guanzhong dairy goats were randomly assigned to 2 groups, one group was injected with 5-HTP intramuscularly and the other group was injected with normal saline. From the first 10 d of the expected date for delivery, 5-HTP or saline was injected into goats through the shoulder muscle every morning before feeding, with a dose of 1\u00a0mg\/kg per body weight. In the in vitro experiment, goat mammary epithelial cells (GMEC) were treated with 100 \u03bcM 5-HT for the evaluation of 5-HT in calcium transportation. The results demonstrated that 5-HTP treatment had no effect on the basic composition of colostrum (P &amp;gt; 0.05) but increased the serum 5-HT concentrations on days \u22125, \u22124, \u22123, and 5 relative to parturition (P &amp;lt; 0.05). The 5-HTP injection group had greater serum calcium concentration on day 4 and greater serum parathyroid hormone-related protein (PTHrP) on days \u22125, \u22124, \u22121, 3, 4, and 5 compared with the saline injection group (P &amp;lt; 0.05). It was further confirmed that 5-HT could increase intracellular calcium levels by increasing PTHrP and decreasing plasma membrane Ca2+-ATPases1 (PMCA1) in GMEC (P &amp;lt; 0.05). In conclusion, 5-HTP treatment in multiparous goats during the transition period from pregnancy to lactation is a feasible way to protect goats from calcium metabolism disorder.<\/jats:p>","DOI":"10.1093\/jas\/skac065","type":"journal-article","created":{"date-parts":[[2022,3,2]],"date-time":"2022-03-02T22:51:05Z","timestamp":1646261465000},"source":"Crossref","is-referenced-by-count":5,"title":["Increasing serotonin concentrations alter calcium metabolism in periparturient dairy goats"],"prefix":"10.1093","volume":"100","author":[{"given":"ZhiFei","family":"Zhang","sequence":"first","affiliation":[{"name":"Laboratory of Genetic Improvement and Healthy Breeding of Dairy Goats, Shaanxi Key Laboratory of Molecular Biology for Agriculture, College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, People\u2019s Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei","family":"Du","sequence":"additional","affiliation":[{"name":"Laboratory of Genetic Improvement and Healthy Breeding of Dairy Goats, Shaanxi Key Laboratory of Molecular Biology for Agriculture, College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, People\u2019s Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"WenYi","family":"Liu","sequence":"additional","affiliation":[{"name":"Laboratory of Genetic Improvement and Healthy Breeding of Dairy Goats, Shaanxi Key Laboratory of Molecular Biology for Agriculture, College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, People\u2019s Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Braden T","family":"Wong","sequence":"additional","affiliation":[{"name":"Department of Animal Science, University of California, Davis, CA 95616, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"HuiLing","family":"Zheng","sequence":"additional","affiliation":[{"name":"Laboratory of Genetic Improvement and Healthy Breeding of Dairy Goats, Shaanxi Key Laboratory of Molecular Biology for Agriculture, College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, People\u2019s Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2022,3,2]]},"reference":[{"issue":"1","key":"2022032913363297800_CIT0001","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1016\/j.coph.2011.02.004","article-title":"5-HT and the immune system.","volume":"11","author":"Ahern","year":"2011","journal-title":"Curr. 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