{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T21:45:50Z","timestamp":1788903950894,"version":"build-2803163510"},"reference-count":53,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[2022,3,16]],"date-time":"2022-03-16T00:00:00Z","timestamp":1647388800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"name":"Natural Sciences and Engineering Council of Canada","award":["RGPIN-2017-04967"],"award-info":[{"award-number":["RGPIN-2017-04967"]}]},{"DOI":"10.13039\/501100001805","name":"Canada Foundation for Innovation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001805","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010318","name":"University of Manitoba","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100010318","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,4,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>The calcium-sensing receptor (CaSR) is a kokumi receptor that plays an essential role in nutrient sensing and animal physiology, growth, and development. Pig CaSR (pCaSR) was identified and characterized in the intestine. However, further research is still needed to confirm the expression of CaSR in the epithelial cells isolated from weaned piglets. In this study, primary enterocytes were isolated and characterized from the ileum of weaned piglets by the Weiser distended intestinal sac technique and fluorescence-activated cell sorting (FACS) based on sucrase-isomaltase (SI) as an enterocyte-specific marker. The expression of CaSR was investigated in both primary enterocytes and the intestinal porcine enterocyte cell line-j2 (IPEC-J2) by droplet digital polymerase chain reaction (ddPCR), immunofluorescence staining, and Western blotting. Results demonstrated that porcine enterocytes could be obtained using FACS with the SI as the enterocyte-specific marker and that pCaSR is not expressed in both porcine ileal enterocytes and IPEC-J2 cells, which specifically identified the expression of pCaSR in ileal enterocytes with sensitive and specific approaches.<\/jats:p>","DOI":"10.1093\/jas\/skac085","type":"journal-article","created":{"date-parts":[[2022,3,16]],"date-time":"2022-03-16T16:50:47Z","timestamp":1647449447000},"source":"Crossref","is-referenced-by-count":2,"title":["Calcium-sensing receptor is not expressed in the absorptive enterocytes of weaned piglets"],"prefix":"10.1093","volume":"100","author":[{"given":"Xiaoya","family":"Zhao","sequence":"first","affiliation":[{"name":"Department of Animal Science, University of Manitoba, Winnipeg, MB R3T 2N2, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qianru","family":"Hui","sequence":"additional","affiliation":[{"name":"Department of Animal Science, University of Manitoba, Winnipeg, MB R3T 2N2, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paula","family":"Azevedo","sequence":"additional","affiliation":[{"name":"Department of Animal Science, University of Manitoba, Winnipeg, MB R3T 2N2, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charles Martin","family":"Nyachoti","sequence":"additional","affiliation":[{"name":"Department of Animal Science, University of Manitoba, Winnipeg, MB R3T 2N2, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karmin","family":"O","sequence":"additional","affiliation":[{"name":"Department of Animal Science, University of Manitoba, Winnipeg, MB R3T 2N2, Canada"},{"name":"CCARM, St. Boniface Hospital Research Centre, Winnipeg, MB R2H 2A6, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chengbo","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Animal Science, University of Manitoba, Winnipeg, MB R3T 2N2, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2022,3,16]]},"reference":[{"key":"2022040717320413800_CIT0001","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1016\/j.jsbmb.2015.02.006","article-title":"Active vitamin D potentiates the anti-neoplastic effects of calcium in the colon: a cross talk through the calcium-sensing receptor","volume":"155","author":"Aggarwal","year":"2016","journal-title":"J. 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