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Ruminally and duodenally cannulated Nellore steers (n = 6; 354 \u00b1 12 kg) were used in a 6 \u00d7 6 repeated switchback design balanced for residual effects. Treatments were arranged in a 3 \u00d7 3 factorial of three nitrogen (N) supplements (urea plus soybean meal; corn gluten meal; dried distillers\u2019 grains plus solubles) and three microbial markers (OCFA; double-labeled urea, 15N; microbial nucleic acid bases, MNAB). The total mixed ration was composed of fresh chopped sugarcane as the forage source in an 83:17 concentrate: forage ratio (dry matter basis). Linear regression was used to develop predictions of MicN from OCFA using 15N and MNAB as response variables. Microbial N flow was underestimated by the MNAB marker compared to 15N. Neither NS nor their respective interactions with the marker methods (MM) affected MicN or EMNS (P &amp;gt; 0.05). However, MicN was different for 15N and MNAB (P &amp;gt; 0.001 for both treatments). Marker methods affected EMNS in all energetic bases (total digestible carbohydrates P &amp;lt; 0.001; rumen-fermentable carbohydrates P &amp;lt; 0.001; organic matter truly degradable in the rumen P &amp;lt; 0.001). Equations that utilized OCFA as a regressor to predict MicN under different MM resulted in good fits of the data as observed by the coefficient of determination (R2; 15N = 0.78; MNAB = 0.69). Microbial N flow estimated from OCFA was overpredicted (15N by 7.46%; MNAB by 4.30%) compared with observed values. The OCFA model presented a small slope bias when methodological validation was applied (15N = 0.96%; MNAB = 3.90%), ensuring reliability of the proposed alternative method. Based on the conditions of this experiment, OCFA may be a suitable alternative to other methods that quantify MicN under different dietary conditions.<\/jats:p>","DOI":"10.1093\/jas\/skac089","type":"journal-article","created":{"date-parts":[[2022,3,21]],"date-time":"2022-03-21T08:23:02Z","timestamp":1647850982000},"source":"Crossref","is-referenced-by-count":0,"title":["Odd-chain fatty acids as an alternative method to predict ruminal microbial nitrogen flow of feedlot Nellore steers fed grain-based diets supplemented with different nitrogen sources"],"prefix":"10.1093","volume":"100","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3726-5670","authenticated-orcid":false,"given":"Let\u00edcia M","family":"Campos","sequence":"first","affiliation":[{"name":"Department of Animal Science, S\u00e3o Paulo State University , Jaboticabal, SP 14884-900 ,","place":["Brazil"]},{"name":"Department of Dairy Science, Virginia Polytechnic Institute and State University , Blacksburg, VA 24060 ,","place":["USA"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8893-4451","authenticated-orcid":false,"given":"Vin\u00edcius C","family":"Souza","sequence":"additional","affiliation":[{"name":"Department of Animal Science, S\u00e3o Paulo State University , Jaboticabal, SP 14884-900 ,","place":["Brazil"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yury T","family":"Granja-Salcedo","sequence":"additional","affiliation":[{"name":"Department of Animal Science, S\u00e3o Paulo State University , Jaboticabal, SP 14884-900 ,","place":["Brazil"]},{"name":"Corporaci\u00f3n Colombiana de Investigaci\u00f3n Agropecuaria \u2013 AGROSAVIA, CIEI Nus , San Roque, Antioqu\u00eda 250047 ,","place":["Colombia"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7462-9010","authenticated-orcid":false,"given":"Juliana D","family":"Messana","sequence":"additional","affiliation":[{"name":"Department of Animal Science, S\u00e3o Paulo State University , Jaboticabal, SP 14884-900 ,","place":["Brazil"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4613-9724","authenticated-orcid":false,"given":"Jacquelyn M","family":"Prestegaard-Wilson","sequence":"additional","affiliation":[{"name":"Department of Dairy Science, Virginia Polytechnic Institute and State University , Blacksburg, VA 24060 ,","place":["USA"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5661-5712","authenticated-orcid":false,"given":"Maria J\u00falia G","family":"Ganga","sequence":"additional","affiliation":[{"name":"Department of Animal Science, S\u00e3o Paulo State University , Jaboticabal, SP 14884-900 ,","place":["Brazil"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana Veronica L","family":"Dias","sequence":"additional","affiliation":[{"name":"Department of Animal Science, S\u00e3o Paulo State University , Jaboticabal, SP 14884-900 ,","place":["Brazil"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3889-7514","authenticated-orcid":false,"given":"Vladimir E","family":"Costa","sequence":"additional","affiliation":[{"name":"Department of Physics and Biophysics, S\u00e3o Paulo State University , Botucatu, SP 18618-689 ,","place":["Brazil"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Telma T","family":"Berchielli","sequence":"additional","affiliation":[{"name":"Department of Animal Science, S\u00e3o Paulo State University , Jaboticabal, SP 14884-900 ,","place":["Brazil"]},{"name":"INCT\/CA\u2013UFV-Department of Animal Science , Vi\u00e7osa, MG 36580-900 ,","place":["Brazil"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2022,3,21]]},"reference":[{"key":"2026041420212340300_CIT0001","first-page":"72","article-title":"In vivo methods to measure digestibility and digestion kinetics of feed fractions in the rumen.","volume-title":"Proceedings of 1st simp\u00f3sio internacional de avan\u00e7os em t\u00e9cnicas de pesquisa em nutri\u00e7\u00e3o de ruminantes","author":"Allen","year":"2007"},{"key":"2026041420212340300_CIT0002","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1079\/ASC50390057","article-title":"Validation of purine bases as a microbial marker by 15 N labelling in growing lambs given high-concentrate diets: effects of grain processing, animal age, and digesta sampling site","volume":"81","author":"Askar","year":"2005","journal-title":"Anim. 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