{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T00:55:14Z","timestamp":1789520114060,"version":"build-2803163510"},"reference-count":47,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2022,6,1]],"date-time":"2022-06-01T00:00:00Z","timestamp":1654041600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/501100005712","name":"University of Jordan","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100005712","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100011755","name":"National Center for Research and Development","doi-asserted-by":"publisher","award":["NEB26-202\/W3112"],"award-info":[{"award-number":["NEB26-202\/W3112"]}],"id":[{"id":"10.13039\/501100011755","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100011755","name":"National Center for Research and Development","doi-asserted-by":"publisher","award":["NEB-26-252\/W4112"],"award-info":[{"award-number":["NEB-26-252\/W4112"]}],"id":[{"id":"10.13039\/501100011755","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,6,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Beef cows with excess androstenedione (A4; High A4) in follicular fluid (FF) and secreted by the ovarian cortex have been reported from the University of Nebraska\u2013Lincoln physiology herd displaying characteristics reminiscent of polycystic ovary syndrome (PCOS). Thus, we hypothesized that naturally occurring High A4 cows were present in other dairy and beef herds. Fourteen Jordan (Amman, Jordon) dairy heifers and 16 U.S. Meat Animal Research Center beef heifers were classified by FF (High A4: A4 &amp;gt; 40 ng\/mL and Control: A4 &amp;lt; 20 ng\/mL) and\/or cortex culture media (High A4 &amp;gt; 1 ng\/mL\/d or Control &amp;lt; 1 ng\/mL\/d). High A4 dairy heifers (n = 6) had greater A4 concentrations (7.6-fold) in FF and (98-fold) greater in ovarian cortex culture media with greater numbers of primordial and fewer later-stage follicles than Controls (n = 8) even after 7 d of culture. Also, the ovarian cortex had greater staining for Picro Sirius red in High A4 dairy heifers compared with Controls indicating increased fibrosis. Thecal cells from High A4 dairy heifers had greater STAR, LHCGR, CYP17A, CD68, and PECAM mRNA expression with increased mRNA abundance of CYP17A1 and CD68 in the ovarian cortex cultures compared with Control dairy heifers. Similarly, cortex culture media from High A4 beef heifers (n = 10) had increased A4 (290-fold; P \u2264 0.001), testosterone (1,427-fold; P \u2264 0.001), and progesterone (9-fold; P \u2264 0.01) compared with Control heifers with increased primordial follicles and decreased later-stage follicles even after 7 d of culture, indicating abnormal follicular development. High A4 ovarian cortex cultures from beef heifers also had increased fibrosis markers and greater expression of PECAM (P = 0.01) with a tendency for increased vascular endothelial cadherin compared with Controls (n = 6). These two trials support our hypothesis that naturally occurring androgen excess cows are present in other dairy and beef herds. The ability to identify these females that have excess A4 ovarian microenvironments may allow for their use in understanding factors causing abnormal follicle development linked to androgen excess and inflammation.<\/jats:p>","DOI":"10.1093\/jas\/skac151","type":"journal-article","created":{"date-parts":[[2022,6,1]],"date-time":"2022-06-01T13:55:44Z","timestamp":1654091744000},"source":"Crossref","is-referenced-by-count":14,"title":["Naturally occurring androgen excess cows are present in dairy and beef herds and have similar characteristics to women with PCOS"],"prefix":"10.1093","volume":"100","author":[{"given":"Mohamed A","family":"Abedal-Majed","sequence":"first","affiliation":[{"name":"Department of Animal Production, School of Agriculture, The University of Jordan , Amman , Jordan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shelby A","family":"Springman","sequence":"additional","affiliation":[{"name":"Department of Animal Science, University of Nebraska\u2013Lincoln , Lincoln , NE 68583 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hanan D","family":"Jafar","sequence":"additional","affiliation":[{"name":"Cell Therapy Center, The University of Jordan , Amman , Jordan"},{"name":"Department of Anatomy and Histology, School of Medicine, The University of Jordan , Amman , Jordan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brooke E","family":"Bell","sequence":"additional","affiliation":[{"name":"Department of Animal Science, University of Nebraska\u2013Lincoln , Lincoln , NE 68583 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Scott G","family":"Kurz","sequence":"additional","affiliation":[{"name":"Department of Animal Science, University of Nebraska\u2013Lincoln , Lincoln , NE 68583 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kyle E","family":"Wilson","sequence":"additional","affiliation":[{"name":"Department of Animal Science, University of Nebraska\u2013Lincoln , Lincoln , NE 68583 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7035-0426","authenticated-orcid":false,"given":"Andrea S","family":"Cupp","sequence":"additional","affiliation":[{"name":"Department of Animal Science, University of Nebraska\u2013Lincoln , Lincoln , NE 68583 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2022,6,1]]},"reference":[{"key":"2022060113543188600_CIT0001","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1677\/joe.0.1740001","article-title":"Developmental origin of polycystic ovary syndrome \u2013 a hypothesis","volume":"174","author":"Abbott","year":"2002","journal-title":"J. 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