{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T23:42:37Z","timestamp":1790725357840,"version":"4.1.0"},"reference-count":56,"publisher":"Oxford University Press (OUP)","issue":"8","license":[{"start":{"date-parts":[[2022,5,13]],"date-time":"2022-05-13T00:00:00Z","timestamp":1652400000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,8,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The first objective was to investigate the effects of feeding rumen-protected methionine (RPM) during a heat stress (HS) challenge on abundance and phosphorylation of mechanistic target of rapamycin (mTOR)-related signaling proteins in mammary gland. The second objective was to investigate how HS and RPM may modulate the response of mammary gland explants to an inflammatory challenge using lipopolysaccharide (LPS). Thirty-two multiparous, lactating Holstein cows (184 \u00b1 59 DIM) were randomly assigned to 1 of 2 environmental treatment groups, and 1 of 2 dietary treatments [TMR with RPM (Smartamine M; Adisseo Inc.; 0.105% DM as top dress) or TMR without RPM (CON)] in a crossover design. There were two periods with two phases per period. In phase 1 (9 d), all cows were in thermoneutral conditions (TN) and fed ad libitum. During phase 2 (9 d), group 1 (n = 16) cows were exposed to HS using electric heat blankets, whereas group 2 cows (n = 16) remained in TN but were pair-fed to HS counterparts to control for DMI decreases associated with HS. After a washout period (14 d), the study was repeated (period 2). Environmental treatments were inverted in period 2 (sequence), whereas dietary treatments remained the same. Mammary tissue was harvested via biopsy at the end of both periods. Tissue was used for protein abundance analysis and also for incubation with 0 or 3 \u03bcg\/mL of LPS for 2 h and subsequently used for mRNA abundance. Data were analyzed using PROC MIXED in SAS. Analysis of protein abundance data included the effects of diet, environment and their interaction, and period and sequence to account for the crossover design. The explant data model also included the effect of LPS and its interaction with environment and diet. Abundance of phosphorylated mTOR and ratio of phosphorylated eukaryotic translation elongation factor 2 (p-EEF2) to total EEF2 in non-challenged tissue was greater with RPM supplementation (P = 0.04 for both) and in both cases tended to be greater with HS (P = 0.08 for both). Regardless of RPM supplementation, incubation with LPS upregulated mRNA abundance of IL8, IL6, IL1B, CXCL2, TNF, NFKB1, and TLR2 (P &amp;lt; 0.05). An environment \u00d7 LPS interaction was observed for NFKB1 (P = 0.03); abundance was greater in LPS-treated explants from non-HS compared with HS cows. Abundance of CXCL2, NFKB1, NOS2, NOS1, and SOD2 was lower with HS (P &amp;lt; 0.05). Although LPS did not alter mRNA abundance of the antioxidant transcription factor NFE2L2 (P = 0.59), explants from HS cows had lower abundance of NFE2L2 (P\u2009&amp;lt;\u20090.001) and CUL3 (P\u2009=\u20090.04). Overall, RPM supplementation may alter mTOR activation in mammary tissue. Additionally, although HS reduced explant immune and antioxidant responses, RPM did not attenuate the inflammatory response induced by LPS in vitro.<\/jats:p>","DOI":"10.1093\/jas\/skac175","type":"journal-article","created":{"date-parts":[[2022,5,13]],"date-time":"2022-05-13T11:48:54Z","timestamp":1652442534000},"source":"Crossref","is-referenced-by-count":18,"title":["Increased supply of methionine during a heat-stress challenge in lactating holstein cows alters mammary tissue mTOR signaling and its response to lipopolysaccharide"],"prefix":"10.1093","volume":"100","author":[{"given":"Danielle Nicole","family":"Coleman","sequence":"first","affiliation":[{"name":"Department of Animal Sciences, University of Illinois , Urbana, IL 61801 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mario","family":"Vailati-Riboni","sequence":"additional","affiliation":[{"name":"Department of Animal Sciences, University of Illinois , Urbana, IL 61801 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Russell T","family":"Pate","sequence":"additional","affiliation":[{"name":"Department of Animal Sciences, University of Illinois , Urbana, IL 61801 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahmad","family":"Aboragah","sequence":"additional","affiliation":[{"name":"Department of Animal Sciences, University of Illinois , Urbana, IL 61801 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"Luchini","sequence":"additional","affiliation":[{"name":"Adisseo , Alpharetta, GA 30022 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1025-3231","authenticated-orcid":false,"given":"Felipe C","family":"Cardoso","sequence":"additional","affiliation":[{"name":"Department of Animal Sciences, University of Illinois , Urbana, IL 61801 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1586-4365","authenticated-orcid":false,"given":"Juan J","family":"Loor","sequence":"additional","affiliation":[{"name":"Department of Animal Sciences, University of Illinois , Urbana, IL 61801 USA"},{"name":"Division of Nutritional Sciences, University of Illinois , Urbana, IL 61801 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2022,5,13]]},"reference":[{"key":"2022081814445757800_CIT0001","doi-asserted-by":"crossref","first-page":"684","DOI":"10.3168\/jds.2018-15128","article-title":"Technical note: developing a heat stress model in dairy cows using an electric heat blanket","volume":"102","author":"Al-Qaisi","year":"2019","journal-title":"J. Dairy Sci"},{"key":"2022081814445757800_CIT0002","doi-asserted-by":"crossref","first-page":"1640","DOI":"10.3945\/jn.117.251876","article-title":"Placentome nutrient transporters and mammalian target of rapamycin signaling proteins are altered by the methionine supply during late gestation in dairy cows and are associated with newborn birth weight","volume":"147","author":"Batistel","year":"2017","journal-title":"J. Nutr"},{"key":"2022081814445757800_CIT0003","doi-asserted-by":"crossref","first-page":"480","DOI":"10.3168\/jds.2017-13185","article-title":"Ethyl-cellulose rumen-protected methionine alleviates inflammation and oxidative stress and improves neutrophil function during the periparturient period and early lactation in Holstein dairy cows","volume":"101","author":"Batistel","year":"2018","journal-title":"J. Dairy Sci"},{"key":"2022081814445757800_CIT0004","doi-asserted-by":"crossref","first-page":"311","DOI":"10.1146\/annurev-animal-031412-103644","article-title":"Effects of heat stress on postabsorptive metabolism and energetics","volume":"1","author":"Baumgard","year":"2013","journal-title":"Annu. Rev. Anim. Biosci"},{"key":"2022081814445757800_CIT0005","doi-asserted-by":"crossref","first-page":"401","DOI":"10.1111\/jpn.12379","article-title":"Heat stress effects on livestock: molecular, cellular and metabolic aspects, a review","volume":"100","author":"Belhadj Slimen","year":"2016","journal-title":"J. Anim. Physiol. Anim. Nutr. (Berl)"},{"key":"2022081814445757800_CIT0006","doi-asserted-by":"crossref","first-page":"3605","DOI":"10.3168\/jds.S0022-0302(03)73966-6","article-title":"Effects of body surface area estimates on predicted energy requirements and heat stress","volume":"86","author":"Berman","year":"2003","journal-title":"J. Dairy Sci"},{"key":"2022081814445757800_CIT0007","doi-asserted-by":"crossref","first-page":"2173","DOI":"10.3168\/jds.S0022-0302(02)74296-3","article-title":"Markers of oxidative status in plasma and erythrocytes of transition dairy cows during hot season","volume":"85","author":"Bernabucci","year":"2002","journal-title":"J. Dairy Sci"},{"key":"2022081814445757800_CIT0008","doi-asserted-by":"crossref","first-page":"1167","DOI":"10.1017\/S175173111000090X","article-title":"Metabolic and hormonal acclimation to heat stress in domesticated ruminants","volume":"4","author":"Bernabucci","year":"2010","journal-title":"Animal"},{"key":"2022081814445757800_CIT0009","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1097\/WOX.0b013e3182439613","article-title":"Oxidative stress and antioxidant defense","volume":"5","author":"Birben","year":"2012","journal-title":"World Allergy Organ. J"},{"key":"2022081814445757800_CIT0010","doi-asserted-by":"crossref","first-page":"1636s","DOI":"10.1093\/jn\/136.6.1636S","article-title":"The sulfur-containing amino acids: an overview","volume":"136","author":"Brosnan","year":"2006","journal-title":"J. Nutr"},{"key":"2022081814445757800_CIT0011","doi-asserted-by":"crossref","first-page":"705","DOI":"10.1016\/j.bcp.2012.11.016","article-title":"The Nrf2 cell defence pathway: Keap1-dependent and -independent mechanisms of regulation","volume":"85","author":"Bryan","year":"2013","journal-title":"Biochem. Pharmacol"},{"key":"2022081814445757800_CIT0012","doi-asserted-by":"crossref","first-page":"711","DOI":"10.13031\/2013.34325","article-title":"Black globe-humidity index (BGHI) as comfort equation for dairy cows","volume":"24","author":"Buffington","year":"1981","journal-title":"Trans. ASAE"},{"key":"2022081814445757800_CIT0013","doi-asserted-by":"crossref","first-page":"1812","DOI":"10.1111\/febs.13627","article-title":"NFKB1: a suppressor of inflammation, ageing and cancer","volume":"283","author":"Cartwright","year":"2016","journal-title":"FEBS J"},{"key":"2022081814445757800_CIT0014","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1016\/j.fct.2013.07.008","article-title":"Glutathione defense mechanism in liver injury: insights from animal models","volume":"60","author":"Chen","year":"2013","journal-title":"Food Chem. Toxicol"},{"key":"2022081814445757800_CIT0015","doi-asserted-by":"crossref","first-page":"676","DOI":"10.3168\/jds.2019-16631","article-title":"Methionine and arginine supplementation alter inflammatory and oxidative stress responses during lipopolysaccharide challenge in bovine mammary epithelial cells in vitro","volume":"103","author":"Dai","year":"2020","journal-title":"J. Dairy Sci"},{"key":"2022081814445757800_CIT0016","doi-asserted-by":"crossref","first-page":"5647","DOI":"10.3168\/jds.2018-15721","article-title":"Effect of heat stress during early, late, and entire dry period on dairy cattle","volume":"102","author":"Fabris","year":"2019","journal-title":"J. Dairy Sci"},{"key":"2022081814445757800_CIT0017","doi-asserted-by":"crossref","first-page":"5040","DOI":"10.3168\/jds.2016-11913","article-title":"The effects of heat stress on protein metabolism in lactating Holstein cows","volume":"100","author":"Gao","year":"2017","journal-title":"J. Dairy Sci"},{"key":"2022081814445757800_CIT0018","doi-asserted-by":"crossref","first-page":"400","DOI":"10.1152\/physiolgenomics.00039.2019","article-title":"Heat stress negatively affects the transcriptome related to overall metabolism and milk protein synthesis in mammary tissue of midlactating dairy cows","volume":"51","author":"Gao","year":"2019","journal-title":"Physiol. Genomics"},{"key":"2022081814445757800_CIT0019","doi-asserted-by":"crossref","first-page":"813","DOI":"10.1126\/science.aao3265","article-title":"SAMTOR is an S-adenosylmethionine sensor for the mTORC1 pathway","volume":"358","author":"Gu","year":"2017","journal-title":"Science (New York, N.Y.)"},{"key":"2022081814445757800_CIT0020","doi-asserted-by":"crossref","first-page":"8505","DOI":"10.3168\/jds.2017-14206","article-title":"Methionine supply alters mammary gland antioxidant gene networks via phosphorylation of nuclear factor erythroid 2-like 2 (NFE2L2) protein in dairy cows during the periparturient period","volume":"101","author":"Han","year":"2018","journal-title":"J. Dairy Sci"},{"key":"2022081814445757800_CIT0021","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1007\/s12192-014-0530-7","article-title":"Methionine protects against hyperthermia-induced cell injury in cultured bovine mammary epithelial cells","volume":"20","author":"Han","year":"2015","journal-title":"Cell Stress Chaperones"},{"key":"2022081814445757800_CIT0022","doi-asserted-by":"crossref","first-page":"e0142633","DOI":"10.1371\/journal.pone.0142633","article-title":"Insulin sensitivity in adipose and skeletal muscle tissue of dairy cows in response to dietary energy level and 2,4-thiazolidinedione (TZD)","volume":"10","author":"Hosseini","year":"2015","journal-title":"PLoS One"},{"key":"2022081814445757800_CIT0023","doi-asserted-by":"crossref","first-page":"448","DOI":"10.1038\/ni.3153","article-title":"IL-6 as a keystone cytokine in health and disease","volume":"16","author":"Hunter","year":"2015","journal-title":"Nat. Immunol"},{"key":"2022081814445757800_CIT0024","doi-asserted-by":"crossref","first-page":"1979","DOI":"10.1007\/s00438-015-1055-1","article-title":"Cross talk between cytokine and hyperthermia-induced pathways: identification of different subsets of NF-\u03baB-dependent genes regulated by TNF\u03b1 and heat shock","volume":"290","author":"Janus","year":"2015","journal-title":"Mol. Genet. Genomics"},{"key":"2022081814445757800_CIT0025","doi-asserted-by":"crossref","first-page":"7130","DOI":"10.1128\/MCB.24.16.7130-7139.2004","article-title":"Oxidative stress sensor Keap1 functions as an adaptor for Cul3-based E3 ligase to regulate proteasomal degradation of Nrf2","volume":"24","author":"Kobayashi","year":"2004","journal-title":"Mol. Cell. Biol"},{"key":"2022081814445757800_CIT0026","doi-asserted-by":"crossref","first-page":"3589","DOI":"10.1242\/jcs.051011","article-title":"mTOR signaling at a glance","volume":"122","author":"Laplante","year":"2009","journal-title":"J. Cell Sci"},{"key":"2022081814445757800_CIT0027","doi-asserted-by":"crossref","first-page":"10477","DOI":"10.3168\/jds.2020-18638","article-title":"Methyl donor supply to heat stress-challenged polymorphonuclear leukocytes from lactating Holstein cows enhances 1-carbon metabolism, immune response, and cytoprotective gene network abundance","volume":"103","author":"Lopreiato","year":"2020","journal-title":"J. Dairy Sci"},{"key":"2022081814445757800_CIT0028","doi-asserted-by":"crossref","first-page":"1957","DOI":"10.1089\/ars.2010.3586","article-title":"Glutathione peroxidase-1 in health and disease: from molecular mechanisms to therapeutic opportunities","volume":"15","author":"Lubos","year":"2011","journal-title":"Antioxid Redox Signal"},{"key":"2022081814445757800_CIT0029","doi-asserted-by":"crossref","first-page":"723","DOI":"10.1038\/nrendo.2014.171","article-title":"Branched-chain amino acids in metabolic signalling and insulin resistance","volume":"10","author":"Lynch","year":"2014","journal-title":"Nat. Rev. Endocrinol"},{"key":"2022081814445757800_CIT0030","doi-asserted-by":"crossref","first-page":"4264","DOI":"10.3168\/jds.2018-15451","article-title":"Phosphorylation of AKT serine\/threonine kinase and abundance of milk protein synthesis gene networks in mammary tissue in response to supply of methionine in periparturient Holstein cows","volume":"102","author":"Ma","year":"2019","journal-title":"J. Dairy Sci"},{"key":"2022081814445757800_CIT0031","doi-asserted-by":"crossref","first-page":"9360","DOI":"10.3168\/jds.2018-14549","article-title":"Nutrient-sensing kinase signaling in bovine immune cells is altered during the postpartum nutrient deficit: a possible role in transition cow inflammatory response","volume":"101","author":"Mann","year":"2018","journal-title":"J. Dairy Sci"},{"key":"2022081814445757800_CIT0032","doi-asserted-by":"crossref","first-page":"a033092","DOI":"10.1101\/cshperspect.a033092","article-title":"Protein synthesis initiation in eukaryotic cells","volume":"10","author":"Merrick","year":"2018","journal-title":"Cold Spring Harb. Perspect. Biol"},{"key":"2022081814445757800_CIT0033","doi-asserted-by":"crossref","first-page":"296","DOI":"10.1016\/j.bbrc.2016.01.185","article-title":"Long-term heat stress induces the inflammatory response in dairy cows revealed by plasma proteome analysis","volume":"471","author":"Min","year":"2016","journal-title":"Biochem. Biophys. Res. Commun"},{"key":"2022081814445757800_CIT0034","doi-asserted-by":"crossref","first-page":"1788","DOI":"10.1128\/MCB.01457-14","article-title":"Elongation factor 2 kinase is regulated by proline hydroxylation and protects cells during hypoxia","volume":"35","author":"Moore","year":"2015","journal-title":"Mol. Cell. Biol"},{"key":"2022081814445757800_CIT0035","volume-title":"Stress proteins in biology and medicine","author":"Morimoto","year":"1990"},{"key":"2022081814445757800_CIT0036","doi-asserted-by":"crossref","first-page":"971","DOI":"10.1002\/cbin.10271","article-title":"Cytoprotection of methionine on hyperthermia-induced damage in bovine mammary epithelial cells","volume":"38","author":"Mu","year":"2014","journal-title":"Cell Biol. Int"},{"key":"2022081814445757800_CIT0037","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1146\/annurev-physiol-020518-114455","article-title":"Branched chain amino acids","volume":"81","author":"Neinast","year":"2019","journal-title":"Annu. Rev. Physiol"},{"key":"2022081814445757800_CIT0038","doi-asserted-by":"crossref","first-page":"254","DOI":"10.3390\/cells7120254","article-title":"Modulation of protein synthesis by eIF2\u03b1 phosphorylation protects cell from heat stress-mediated apoptosis","volume":"7","author":"Park","year":"2018","journal-title":"Cells"},{"key":"2022081814445757800_CIT0039","doi-asserted-by":"crossref","first-page":"2800","DOI":"10.3168\/jds.2019-17305","article-title":"Effects of rumen-protected methionine on lactation performance and physiological variables during a heat stress challenge in lactating Holstein cows","volume":"103","author":"Pate","year":"2020","journal-title":"J. Dairy Sci"},{"issue":"12","key":"2022081814445757800_CIT0040","doi-asserted-by":"crossref","first-page":"skab323","DOI":"10.1093\/jas\/skab323","article-title":"Immune and metabolic effects of rumen-protected methionine during a heat stress challenge in lactating Holstein cows","volume":"99","author":"Pate","year":"2021","journal-title":"J. Anim. Sci"},{"key":"2022081814445757800_CIT0041","doi-asserted-by":"crossref","first-page":"39","DOI":"10.15232\/aas.2018-01805","article-title":"Adaptations of protein and amino acid metabolism to heat stress in dairy cows and other livestock species.","volume":"35","author":"R\u00edus","year":"2019","journal-title":"Appl. Anim. Sci"},{"key":"2022081814445757800_CIT0042","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1089\/107999003321455507","article-title":"The primary role in biologic activity of the neutrophil chemokines IL-8 and GRO-alpha in cultured nasal epithelial cells","volume":"23","author":"Rudack","year":"2003","journal-title":"J. Interferon Cytokine Res"},{"key":"2022081814445757800_CIT0043","doi-asserted-by":"crossref","first-page":"e1003471","DOI":"10.1371\/journal.pcbi.1003471","article-title":"Overexpression of heat shock protein 72 attenuates NF-\u03baB activation using a combination of regulatory mechanisms in microglia","volume":"10","author":"Sheppard","year":"2014","journal-title":"PLoS Comput. Biol"},{"key":"2022081814445757800_CIT0044","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1016\/j.freeradbiomed.2015.06.006","article-title":"Molecular basis of the Keap1\u2013Nrf2 system","volume":"88","author":"Suzuki","year":"2015","journal-title":"Free Radic. Biol. Med"},{"key":"2022081814445757800_CIT0045","doi-asserted-by":"crossref","first-page":"6128","DOI":"10.1074\/jbc.M110.166991","article-title":"Amino acids activate mammalian target of rapamycin complex 2 (mTORC2) via PI3K\/Akt signaling","volume":"286","author":"Tato","year":"2011","journal-title":"J. Biol. Chem"},{"key":"2022081814445757800_CIT0046","doi-asserted-by":"crossref","first-page":"1727","DOI":"10.1089\/ars.2017.7342","article-title":"Transcriptional regulation by Nrf2","volume":"29","author":"Tonelli","year":"2018","journal-title":"Antioxid Redox Signal"},{"key":"2022081814445757800_CIT0047","doi-asserted-by":"crossref","first-page":"1057","DOI":"10.3389\/fmicb.2019.01057","article-title":"The role of interleukin 6 during viral infections","volume":"10","author":"Velazquez-Salinas","year":"2019","journal-title":"Front. Microbiol"},{"key":"2022081814445757800_CIT0048","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1042\/BJ20150089","article-title":"Regulated stability of eukaryotic elongation factor 2 kinase requires intrinsic but not ongoing activity","volume":"467","author":"Wang","year":"2015","journal-title":"Biochem. J"},{"key":"2022081814445757800_CIT0049","doi-asserted-by":"crossref","first-page":"4370","DOI":"10.1093\/emboj\/20.16.4370","article-title":"Regulation of elongation factor 2 kinase by p90(RSK1) and p70 S6 kinase","volume":"20","author":"Wang","year":"2001","journal-title":"EMBO J"},{"key":"2022081814445757800_CIT0050","doi-asserted-by":"crossref","first-page":"621","DOI":"10.1042\/BST20150014","article-title":"Dissecting molecular cross-talk between Nrf2 and NF-\u03baB response pathways","volume":"43","author":"Wardyn","year":"2015","journal-title":"Biochem. Soc. Trans"},{"key":"2022081814445757800_CIT0051","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1007\/s00726-008-0210-y","article-title":"Arginine metabolism and nutrition in growth, health and disease","volume":"37","author":"Wu","year":"2009","journal-title":"Amino Acids"},{"key":"2022081814445757800_CIT0052","doi-asserted-by":"crossref","first-page":"405","DOI":"10.3390\/nu8070405","article-title":"The emerging role of branched-chain amino acids in insulin resistance and metabolism","volume":"8","author":"Yoon","year":"2016","journal-title":"Nutrients"},{"key":"2022081814445757800_CIT0053","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3390\/ani10061015","article-title":"Transcriptome functional analysis of mammary gland of cows in heat stress and thermoneutral condition","volume":"10","author":"Yue","year":"2020","journal-title":"Animals"},{"key":"2022081814445757800_CIT0054","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1016\/j.jprot.2017.02.011","article-title":"Seasonal heat stress affects adipose tissue proteome toward enrichment of the Nrf2-mediated oxidative stress response in late-pregnant dairy cows","volume":"158","author":"Zachut","year":"2017","journal-title":"J. Proteomics"},{"key":"2022081814445757800_CIT0055","doi-asserted-by":"crossref","first-page":"8716","DOI":"10.3168\/jds.2015-10525","article-title":"Better postpartal performance in dairy cows supplemented with rumen-protected methionine compared with choline during the peripartal period","volume":"99","author":"Zhou","year":"2016","journal-title":"J. Dairy Sci"},{"key":"2022081814445757800_CIT0056","doi-asserted-by":"crossref","first-page":"8956","DOI":"10.3168\/jds.2016-10986","article-title":"Rumen-protected methionine compared with rumen-protected choline improves immunometabolic status in dairy cows during the peripartal period","volume":"99","author":"Zhou","year":"2016","journal-title":"J. Dairy Sci"}],"container-title":["Journal of Animal Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/jas\/advance-article-pdf\/doi\/10.1093\/jas\/skac175\/43699097\/skac175.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jas\/article-pdf\/100\/8\/skac175\/45476387\/skac175.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jas\/article-pdf\/100\/8\/skac175\/45476387\/skac175.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,2,5]],"date-time":"2023-02-05T04:39:25Z","timestamp":1675571965000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jas\/article\/doi\/10.1093\/jas\/skac175\/6585298"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,5,13]]},"references-count":56,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2022,5,13]]},"published-print":{"date-parts":[[2022,8,1]]}},"URL":"https:\/\/doi.org\/10.1093\/jas\/skac175","relation":{},"ISSN":["0021-8812","1525-3163"],"issn-type":[{"value":"0021-8812","type":"print"},{"value":"1525-3163","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2022,8,1]]},"published":{"date-parts":[[2022,5,13]]},"article-number":"skac175"}}