{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,20]],"date-time":"2026-04-20T23:11:17Z","timestamp":1776726677587,"version":"3.51.2"},"reference-count":31,"publisher":"Oxford University Press (OUP)","issue":"11","license":[{"start":{"date-parts":[[2022,8,4]],"date-time":"2022-08-04T00:00:00Z","timestamp":1659571200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/100012752","name":"Elanco Animal Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100012752","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,11,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Experimental bovine respiratory syncytial virus (BRSV) infection can enhance Histophilus somni (Hs) disease in calves; we thus hypothesized that modified-live virus (MLV) vaccines containing BRSV may alter Hs carriage. Our objective was to determine the effects of an intranasal (IN) trivalent (infectious bovine rhinotracheitis virus [IBRV], parainfluenza-3 virus [PI3V], and BRSV) respiratory vaccine with parenteral (PT) bivalent bovine viral diarrhea virus (BVDV) type I + II vaccine, or a PT pentavalent (BVDV type I and II, IBRV, BRSV, and PI3V) respiratory vaccine, on health, growth, immunity, and nasal pathogen colonization in high-risk beef calves. Calves (n = 525) were received in five truckload blocks and stratified by body weight (213 \u00b1 18.4 kg), sex, and presence of a pre-existing ear-tag. Pens were spatially arranged in sets of three within a block and randomly assigned to treatment with an empty pen between treatment groups consisting of: 1) no MLV respiratory vaccination (CON), 2) IN trivalent MLV respiratory vaccine with PT BVDV type I + II vaccine (INT), or 3) PT pentavalent, MLV respiratory vaccine (INJ). The pen was the experimental unit, with 15 pens\/treatment and 11 to 12 calves\/pen in this 70-d receiving study. Health, performance, and BRSV, Hs, Mycoplasma bovis (Mb), Mannheimia haemolytica (Mh), and Pasteurella multocida (Pm) level in nasal swabs via rtPCR was determined on days 0, 7, 14, and 28, and BRSV-specific serum neutralizing antibody titer, and serum IFN-\u03b3 concentration via ELISA, were evaluated on days 0, 14, 28, 42, 56, and 70. Morbidity (P = 0.83), mortality (P = 0.68) and average daily gain (P \u2265 0.82) did not differ. Serum antibodies against BRSV increased with time (P &amp;lt; 0.01). There was a treatment \u00d7 time interaction (P &amp;lt; 0.01) for Hs detection; on days 14 and 28, INT (21.1% and 57.1%) were more frequently (P &amp;lt; 0.01) Hs positive than CON (3.6% and 25.3%) or INJ (3.4 % and 8.4%). Also, INT had reduced (P = 0.03) cycle time of Hs positive samples on day 28. No difference (P \u2265 0.17) was found for IFN-\u03b3 concentration and Mb, Mh, or Pm detection. The proportion of Mh positive culture from lung specimens differed (P &amp;lt; 0.01); INT had fewer (0.0%; 0 of 9) Mh positive lungs than INJ (45.5%; 6 of 13) or CON (74.0%; 14 of 19). Vaccination of high-risk calves with MLV did not clearly impact health or growth during the receiving period. However, INT was associated with an altered upper respiratory microbial community in cattle resulting in increased detection and level of Hs.<\/jats:p>","DOI":"10.1093\/jas\/skac249","type":"journal-article","created":{"date-parts":[[2022,8,4]],"date-time":"2022-08-04T23:29:51Z","timestamp":1659655791000},"source":"Crossref","is-referenced-by-count":5,"title":["Clinical and microbiological effects in high-risk beef calves administered intranasal or parenteral modified-live virus vaccines"],"prefix":"10.1093","volume":"100","author":[{"given":"Sherri A","family":"Powledge","sequence":"first","affiliation":[{"name":"Department of Agricultural Sciences, West Texas A&M University , Canyon, 79016 TX , USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Taylor B","family":"McAtee","sequence":"additional","affiliation":[{"name":"Department of Agricultural Sciences, West Texas A&M University , Canyon, 79016 TX , USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amelia R","family":"Woolums","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Mississippi State University , Starkville, 39759, MS , USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T","family":"Robin Falkner","sequence":"additional","affiliation":[{"name":"Cattle Flow Consulting , Christiana, 37037 TN , USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John T","family":"Groves","sequence":"additional","affiliation":[{"name":"Livestock Veterinary Service , Eldon, 65026, MO , USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Merilee","family":"Thoresen","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Mississippi State University , Starkville, 39759, MS , USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert","family":"Valeris-Chacin","sequence":"additional","affiliation":[{"name":"VERO, Department of Veterinary Pathobiology, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University , Canyon, 79015 TX , USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6632-8557","authenticated-orcid":false,"given":"John T","family":"Richeson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2022,8,4]]},"reference":[{"key":"2022110917533736900_CIT0001","doi-asserted-by":"crossref","first-page":"1263","DOI":"10.1164\/ajrccm.160.4.9812025","article-title":"Reduced interferon-gamma expression in peripheral blood mononuclear cells of infants with severe respiratory syncytial virus disease","volume":"160","author":"Aberle","year":"1999","journal-title":"Am. 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