{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,20]],"date-time":"2026-04-20T19:47:23Z","timestamp":1776714443856,"version":"3.51.2"},"reference-count":46,"publisher":"Oxford University Press (OUP)","license":[{"start":{"date-parts":[[2022,11,16]],"date-time":"2022-11-16T00:00:00Z","timestamp":1668556800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,1,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>The intestinal morphology and function can be compromised in pigs exposed to heat stress (HS), partly due to increased production of reactive-oxygen species. Because methionine (Met) functions as intracellular antioxidant, the requirement of Met may be increased in HS-pigs. The effect of dietary supplementation with dl-Met above requirement on performance, small intestine morphology, antioxidant enzymes activity, amino acid transporters expression, and serum concentration (SC) of free AA in HS-pigs was evaluated. A basal wheat\u2013soybean meal diet was formulated to meet 100% Met requirement with the other indispensable AA exceeding at least 20% their requirement. Sixty individually housed pigs (23.0 \u00b1 2.4 kg BW, 12 pigs per treatment) were randomly assigned to five treatments: TN100, thermal-neutral (22.7 \u00b0C) housed pigs fed the basal diet; HS100, HS120, HS140, HS160; HS-pigs (29.6 \u00b0C to 39.4 \u00b0C) fed the basal diet supplemented with dl-Met to contain 0%, 20%, 40%, and 60% dl-Met above the requirement, respectively. Pigs had free access to feed and water during the 21-d trial. Blood samples were collected on day 18 to analyze the absorptive AA-SC. The effect of ambient temperature (HS100 vs. TN100), as well as the linear and quadratic effects of increasing Met levels in the diets for HS-pigs were analyzed. The HS100 pigs gained less weight than TN100 and HS120 pigs (P &amp;lt; 0.01); gain:feed was also higher in HS120 pigs than in HS100 pigs (P \u2264 0.05). Feed intake of TN100 pigs was higher than that of HS-pigs fed the dl-Met supplemented diets (P &amp;lt; 0.05). Villi height reduced in pigs HS, but Met supplementation quadratically increased it (P &amp;lt; 0.05). Superoxide dismutase and catalase activities, reduced glutathione concentration, and relative expression of B0AT2 in ileum decreased (P &amp;lt; 0.05), but glutathione peroxidase activity increased in HS-pigs. dl-Met supplementation linearly affected catalase and glutathione peroxidase activities, as well as the relative expression of b0,+AT in jejunum (P &amp;lt; 0.05) of HS-pigs. The SC of Ile, Leu, Lys, Phe, and Val were higher in HS100 pigs than in TN100 pigs (P &amp;lt; 0.05). Graded levels of supplemental dl-Met in diets for HS-pigs linearly decreased SC of Ile, Leu, and Val (P &amp;lt; 0.05), tended to decrease His, Lys, and Thr (P &amp;lt; 0.10), and increased Met (P &amp;lt; 0.01). In conclusion, HS had negative effect on weight gain and intestinal morpho-physiology; however, it was ameliorated by adding 20% Met above the requirement in diets for growing pigs.<\/jats:p>","DOI":"10.1093\/jas\/skac379","type":"journal-article","created":{"date-parts":[[2022,11,16]],"date-time":"2022-11-16T17:28:50Z","timestamp":1668619730000},"source":"Crossref","is-referenced-by-count":14,"title":["Effect of <scp>dl<\/scp>-methionine supplementation above requirement on performance; intestinal morphology, antioxidant activity, and gene expression; and serum concentration of amino acids in heat stressed pigs"],"prefix":"10.1093","volume":"101","author":[{"given":"Adriana","family":"Morales","sequence":"first","affiliation":[{"name":"ICA-Universidad Aut\u00f3noma de Baja California , 21100 Mexicali, B.C. , M\u00e9xico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ver\u00f3nica","family":"S\u00e1nchez","sequence":"additional","affiliation":[{"name":"ICA-Universidad Aut\u00f3noma de Baja California , 21100 Mexicali, B.C. , M\u00e9xico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bayron","family":"P\u00e9rez","sequence":"additional","affiliation":[{"name":"ICA-Universidad Aut\u00f3noma de Baja California , 21100 Mexicali, B.C. , M\u00e9xico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Reyna L","family":"Camacho","sequence":"additional","affiliation":[{"name":"ICA-Universidad Aut\u00f3noma de Baja California , 21100 Mexicali, B.C. , M\u00e9xico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N\u00e9stor","family":"Arce","sequence":"additional","affiliation":[{"name":"ICA-Universidad Aut\u00f3noma de Baja California , 21100 Mexicali, B.C. , M\u00e9xico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ernesto","family":"Avelar","sequence":"additional","affiliation":[{"name":"ICA-Universidad Aut\u00f3noma de Baja California , 21100 Mexicali, B.C. , M\u00e9xico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jollie-Carolline","family":"Gonz\u00e1lez-Vega","sequence":"additional","affiliation":[{"name":"Evonik Operations GmbH, 63457 Hanau , Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John K","family":"Htoo","sequence":"additional","affiliation":[{"name":"Evonik Operations GmbH, 63457 Hanau , Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3713-8305","authenticated-orcid":false,"given":"Miguel","family":"Cervantes","sequence":"additional","affiliation":[{"name":"ICA-Universidad Aut\u00f3noma de Baja California , 21100 Mexicali, B.C. , M\u00e9xico"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2022,11,16]]},"reference":[{"issue":"37","key":"2023112010562821700_CIT0001","first-page":"1","article-title":"Association between heat stress and oxidative stress in poultry; mitochondrial dysfunction and dietary interventions with phytochemicals","volume":"7","author":"Akbarian","year":"2016","journal-title":"J. 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