{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T19:47:06Z","timestamp":1790624826055,"version":"4.1.0"},"reference-count":56,"publisher":"Oxford University Press (OUP)","license":[{"start":{"date-parts":[[2022,11,28]],"date-time":"2022-11-28T00:00:00Z","timestamp":1669593600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,1,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>This study aimed to determine the regulatory mechanism of bone morphogenetic protein 4 (BMP4) gene in the testes of Tibetan sheep and its role in the blood\u2013testis barrier (BTB). First, we cloned BMP4 gene for bioinformatics analysis, and detected the mRNA and protein expression levels of BMP4 in the testes of Tibetan sheep pre-puberty (3-mo-old), during sexual maturity (1-yr-old), and in adulthood (3-yr-old) by qRT-PCR and Western blot. In addition, the subcellular localization of BMP4 was analyzed by immunohistochemical staining. Next, BMP4 overexpression and silencing vectors were constructed and transfected into primary Sertoli cells (SCs) to promote and inhibit the proliferation of BMP4, respectively. Then, CCK-8 was used to detect the proliferation effect of SCs. The expression of BMP4 and downstream genes, pathway receptors, tight junction-related proteins, and cell proliferation and apoptosis-related genes in SCs were studied using qRT-PCR and Western blot. The results revealed that the relative expression of BMP4 mRNA and protein in testicular tissues of 1Y group and 3Y group was dramatically higher than that of 3M group (P &amp;lt; 0.01), and BMP4 protein is mainly located in SCs and Leydig cells at different development stages. The CDS region of the Tibetan sheep BMP4 gene was 1,229 bp. CCK-8 results demonstrated that the proliferation rate of BMP4 was significantly increased in the overexpression group (pc-DNA-3.1(+)-BMP4; P &amp;lt; 0.05). In addition, the mRNA and protein expressions of SMAD5, BMPR1A, and BMPR1B and tight junction-related proteins Claudin11, Occludin, and ZO1 were significantly increased (P &amp;lt; 0.05). The mRNA expression of cell proliferation-related gene Bcl2 was significantly enhanced (P &amp;lt; 0.05), and the expression of GDNF was enhanced (P &amp;gt; 0.05). The mRNA expression of apoptosis-related genes Caspase3 and Bax decreased significantly (P &amp;lt; 0.05), while the mRNA expression of cell cycle-related genes CyclinA2 and CDK2 increased significantly (P &amp;lt; 0.05). It is worth noting that the opposite results were observed after transfection with si-BMP4. In summary, what should be clear from the results reported here is that BMP4 affects testicular development by regulating the Sertoli cells and BTB, thereby modulating the spermatogenesis of Tibetan sheep.<\/jats:p>","DOI":"10.1093\/jas\/skac393","type":"journal-article","created":{"date-parts":[[2022,11,28]],"date-time":"2022-11-28T12:00:07Z","timestamp":1669636807000},"source":"Crossref","is-referenced-by-count":10,"title":["The regulatory role of BMP4 in testicular Sertoli cells of Tibetan sheep"],"prefix":"10.1093","volume":"101","author":[{"given":"Keyan","family":"Ma","sequence":"first","affiliation":[{"name":"College of Animal Science and Technology, Gansu Agricultural University , Lanzhou 730070 , China"},{"name":"Gansu Key Laboratory of Animal Generational Physiology and Reproductive Regulation , Lanzhou 730070 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nana","family":"Chen","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Gansu Agricultural University , Lanzhou 730070 , China"},{"name":"Gansu Key Laboratory of Animal Generational Physiology and Reproductive Regulation , Lanzhou 730070 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huihui","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Gansu Agricultural University , Lanzhou 730070 , China"},{"name":"Gansu Key Laboratory of Animal Generational Physiology and Reproductive Regulation , Lanzhou 730070 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiao","family":"Li","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Gansu Agricultural University , Lanzhou 730070 , China"},{"name":"Gansu Key Laboratory of Animal Generational Physiology and Reproductive Regulation , Lanzhou 730070 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huibin","family":"Shi","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Gansu Agricultural University , Lanzhou 730070 , China"},{"name":"Gansu Key Laboratory of Animal Generational Physiology and Reproductive Regulation , Lanzhou 730070 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manchun","family":"Su","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Gansu Agricultural University , Lanzhou 730070 , China"},{"name":"Gansu Key Laboratory of Animal Generational Physiology and Reproductive Regulation , Lanzhou 730070 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Gansu Agricultural University , Lanzhou 730070 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Youji","family":"Ma","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Gansu Agricultural University , Lanzhou 730070 , China"},{"name":"Gansu Key Laboratory of Animal Generational Physiology and Reproductive Regulation , Lanzhou 730070 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Taotao","family":"Li","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Gansu Agricultural University , Lanzhou 730070 , China"},{"name":"Gansu Key Laboratory of Animal Generational Physiology and Reproductive Regulation , Lanzhou 730070 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2022,11,28]]},"reference":[{"key":"2023112010573774800_CIT0001","doi-asserted-by":"crossref","first-page":"145897","DOI":"10.1016\/j.gene.2021.145897","article-title":"PGAM1 regulates the glycolytic metabolism of SCs in Tibetan sheep and its influence on the development of SCs","volume":"804","author":"An","year":"2021","journal-title":"Gene"},{"key":"2023112010573774800_CIT0002","doi-asserted-by":"crossref","first-page":"2257","DOI":"10.1242\/dev.01125","article-title":"Epithelial Bmpr1a regulates differentiation and proliferation in postnatal hair follicles and is essential for tooth development","volume":"131","author":"Andl","year":"2004","journal-title":"Development"},{"key":"2023112010573774800_CIT0003","doi-asserted-by":"crossref","first-page":"1179","DOI":"10.1016\/S0006-2952(00)00384-1","article-title":"Cyclin A2 transcriptional regulation: modulation of cell cycle control at the G1\/S transition by peripheral cues","volume":"60","author":"Blanchard","year":"2000","journal-title":"Biochem. Pharmacol"},{"key":"2023112010573774800_CIT0004","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1016\/j.abb.2014.07.011","article-title":"Bone morphogenetic proteins: structure, biological function and therapeutic applications","volume":"561","author":"Carreira","year":"2014","journal-title":"Arch. Biochem. Biophys"},{"key":"2023112010573774800_CIT0005","doi-asserted-by":"crossref","first-page":"723","DOI":"10.1038\/nri.2016.112","article-title":"Immunoregulation by members of the TGF\u03b2 superfamily","volume":"16","author":"Chen","year":"2016","journal-title":"Nat. Rev. Immunol"},{"key":"2023112010573774800_CIT0006","doi-asserted-by":"crossref","first-page":"1109","DOI":"10.1002\/2211-5463.12640","article-title":"Bone morphogenetic protein 4 regulates immortalized chicken preadipocyte proliferation by promoting G1\/S cell \u00adcycle progression","volume":"9","author":"Chen","year":"2019","journal-title":"FEBS Open Bio"},{"key":"2023112010573774800_CIT0007","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1124\/pr.110.002790","article-title":"The blood-testis barrier and its implications for male contraception","volume":"64","author":"Cheng","year":"2012","journal-title":"Pharmacol. Rev"},{"key":"2023112010573774800_CIT0008","doi-asserted-by":"crossref","first-page":"1741","DOI":"10.1161\/CIRCRESAHA.107.153544","article-title":"Cyclin A2 induces cardiac regeneration after myocardial infarction and prevents heart failure","volume":"100","author":"Cheng","year":"2007","journal-title":"Circ. Res"},{"key":"2023112010573774800_CIT0009","doi-asserted-by":"crossref","first-page":"415","DOI":"10.1158\/1541-7786.MCR-11-0293","article-title":"The activation of MEK\/ERK signaling pathway by bone morphogenetic protein 4 to increase hepatocellular carcinoma cell proliferation and migration","volume":"10","author":"Chiu","year":"2012","journal-title":"Mol. Cancer Res"},{"key":"2023112010573774800_CIT0010","doi-asserted-by":"crossref","first-page":"237","DOI":"10.2174\/0929867321666141106113633","article-title":"Cyclin-dependent kinase-2 as a target for cancer therapy: progress in the development of CDK2 inhibitors as anti-cancer agents","volume":"22","author":"Chohan","year":"2015","journal-title":"Curr. Med. Chem"},{"key":"2023112010573774800_CIT0011","doi-asserted-by":"crossref","first-page":"17137","DOI":"10.1074\/jbc.R115.636472","article-title":"Actin mechanics and fragmentation","volume":"290","author":"De La Cruz","year":"2015","journal-title":"J. Biol. Chem"},{"key":"2023112010573774800_CIT0012","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1083\/jcb.200511036","article-title":"Regulation of myogenic progenitor proliferation in human fetal skeletal muscle by BMP4 and its antagonist Gremlin","volume":"175","author":"Frank","year":"2006","journal-title":"J. Cell Biol"},{"key":"2023112010573774800_CIT0013","doi-asserted-by":"crossref","first-page":"1463","DOI":"10.1590\/S0100-879X2005001000003","article-title":"Bone morphogenetic proteins: from structure to clinical use","volume":"38","author":"Granjeiro","year":"2005","journal-title":"Braz. J. Med. Biol. Res"},{"key":"2023112010573774800_CIT0014","doi-asserted-by":"crossref","first-page":"1288","DOI":"10.1161\/CIRCRESAHA.120.317439","article-title":"Shaping waves of bone morphogenetic protein inhibition during vascular growth","volume":"127","author":"Guihard","year":"2020","journal-title":"Circ. Res"},{"key":"2023112010573774800_CIT0015","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s12964-015-0101-2","article-title":"Long-term culture and significant expansion of human Sertoli cells whilst maintaining stable global phenotype and AKT and SMAD1\/5 activation","volume":"13","author":"Guo","year":"2015","journal-title":"Cell Commun. Signal"},{"key":"2023112010573774800_CIT0016","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1016\/j.semcdb.2014.04.007","article-title":"The roles and regulation of Sertoli cells in fate determinations of spermatogonial stem cells and spermatogenesis","volume":"29","author":"Hai","year":"2014","journal-title":"Semin. Cell Dev. Biol"},{"key":"2023112010573774800_CIT0017","first-page":"311","article-title":"BMP4 promotes human Sertoli cell proliferation via Smad1\/5 and ID2\/3 pathway and its abnormality is associated with azoospermia","volume":"19","author":"Hai","year":"2015","journal-title":"Discov. Med"},{"key":"2023112010573774800_CIT0018","doi-asserted-by":"crossref","first-page":"806","DOI":"10.1038\/nrc2467","article-title":"Bone morphogenetic protein signalling in colorectal cancer","volume":"8","author":"Hardwick","year":"2008","journal-title":"Nat. Rev. Cancer"},{"key":"2023112010573774800_CIT0019","doi-asserted-by":"crossref","first-page":"2517","DOI":"10.1002\/stem.1486","article-title":"MEK\/ERK signaling directly and indirectly contributes to the cyclical self-renewal of spermatogonial stem cells","volume":"31","author":"Hasegawa","year":"2013","journal-title":"Stem Cells"},{"key":"2023112010573774800_CIT0020","doi-asserted-by":"crossref","first-page":"2230","DOI":"10.1007\/s12010-012-9932-0","article-title":"Growth characteristics of human adipose-derived stem cells during long time culture regulated by Cyclin A and Cyclin D1","volume":"168","author":"Jiang","year":"2012","journal-title":"Appl. Biochem. Biotechnol"},{"key":"2023112010573774800_CIT0021","doi-asserted-by":"crossref","first-page":"567","DOI":"10.1016\/j.stem.2012.06.011","article-title":"Reconstitution of mouse spermatogonial stem cell niches in culture","volume":"11","author":"Kanatsu-Shinohara","year":"2012","journal-title":"Cell Stem Cell"},{"key":"2023112010573774800_CIT0022","doi-asserted-by":"crossref","first-page":"7837","DOI":"10.1073\/pnas.1914963117","article-title":"Autologous transplantation of spermatogonial stem cells restores fertility in congenitally infertile mice","volume":"117","author":"Kanatsu-Shinohara","year":"2020","journal-title":"Proc. Natl. Acad. Sci. U.S.A"},{"key":"2023112010573774800_CIT0023","doi-asserted-by":"crossref","first-page":"96","DOI":"10.1126\/science.270.5233.96","article-title":"Bax-deficient mice with lymphoid hyperplasia and male germ cell death","volume":"270","author":"Knudson","year":"1995","journal-title":"Science"},{"key":"2023112010573774800_CIT0024","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1016\/S0022-1759(02)00073-X","article-title":"Evaluation of caspase activity in apoptotic cells","volume":"265","author":"Kohler","year":"2002","journal-title":"J. Immunol. Methods"},{"key":"2023112010573774800_CIT0025","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1016\/j.cytogfr.2009.07.007","article-title":"Cytokines and junction restructuring events during spermatogenesis in the testis: an emerging concept of regulation","volume":"20","author":"Li","year":"2009","journal-title":"Cytokine Growth Factor Rev"},{"key":"2023112010573774800_CIT0026","doi-asserted-by":"crossref","first-page":"492","DOI":"10.1111\/j.1365-2184.2008.00535.x","article-title":"Ratio of Wnt3a to BMP4 doses is critical to their synergistic effects on proliferation of differentiating mouse embryonic stem cells","volume":"41","author":"Lin","year":"2008","journal-title":"Cell Prolif"},{"key":"2023112010573774800_CIT0027","volume-title":"Selection, identification and SNP analysis of correlation genes with cashmere growth on cashmere goats","author":"Liu","year":"2012"},{"key":"2023112010573774800_CIT0028","doi-asserted-by":"crossref","first-page":"102702","DOI":"10.1016\/j.dnarep.2019.102702","article-title":"To control or to be controlled? Dual roles of CDK2 in DNA damage and DNA damage response","volume":"85","author":"Liu","year":"2020","journal-title":"DNA Repair (Amst.)"},{"key":"2023112010573774800_CIT0029","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of relative gene expression data using real-time quantitative PCR and the 2\u2212\u0394\u0394CT method","volume":"25","author":"Livak","year":"2001","journal-title":"Methods"},{"key":"2023112010573774800_CIT0030","doi-asserted-by":"crossref","first-page":"291","DOI":"10.5483\/BMBRep.2010.43.4.291","article-title":"Suppression of CDK2 expression by siRNA induces cell cycle arrest and cell proliferation inhibition in human cancer cells","volume":"43","author":"Long","year":"2010","journal-title":"BMB Rep"},{"key":"2023112010573774800_CIT0031","doi-asserted-by":"crossref","first-page":"E1928","DOI":"10.1002\/humu.21376","article-title":"Evaluation of germline BMP4 mutation as a cause of colorectal cancer","volume":"32","author":"Lubbe","year":"2011","journal-title":"Hum. Mutat"},{"key":"2023112010573774800_CIT0032","doi-asserted-by":"crossref","first-page":"1087","DOI":"10.1095\/biolreprod.102.010371","article-title":"Sertoli cell tight junction dynamics: their regulation during spermatogenesis","volume":"68","author":"Lui","year":"2003","journal-title":"Biol. Reprod"},{"key":"2023112010573774800_CIT0033","doi-asserted-by":"crossref","first-page":"202","DOI":"10.1095\/biolreprod.109.078907","article-title":"Claudin 11 \u00addeficiency in mice results in loss of the Sertoli cell epithelial phenotype in the testis","volume":"82","author":"Mazaud-Guittot","year":"2010","journal-title":"Biol. Reprod"},{"key":"2023112010573774800_CIT0034","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1038\/ng0197-83","article-title":"Delayed early embryonic lethality following disruption of the murine cyclin A2 gene","volume":"15","author":"Murphy","year":"1997","journal-title":"Nat. Genet"},{"key":"2023112010573774800_CIT0035","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1146\/annurev.cellbio.24.110707.175355","article-title":"Regulation of spermatogonial stem cell self-renewal in mammals","volume":"24","author":"Oatley","year":"2008","journal-title":"Annu. Rev. Cell Dev. Biol"},{"key":"2023112010573774800_CIT0036","doi-asserted-by":"crossref","first-page":"387","DOI":"10.1093\/jb\/mvn176","article-title":"Signalling pathway involving GULP, MAPK and Rac1 for SR-BI-induced phagocytosis of apoptotic cells","volume":"145","author":"Osada","year":"2009","journal-title":"J. Biochem"},{"key":"2023112010573774800_CIT0037","doi-asserted-by":"crossref","first-page":"3363","DOI":"10.1242\/jcs.00650","article-title":"Developmental expression of BMP4\/ALK3\/SMAD5 signaling pathway in the mouse testis: a potential role of BMP4 in spermatogonia differentiation","volume":"116","author":"Pellegrini","year":"2003","journal-title":"J. Cell Sci"},{"key":"2023112010573774800_CIT0038","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1016\/j.reprotox.2016.03.041","article-title":"Zinc attenuates ethanol-induced Sertoli cell toxicity and apoptosis through caspase-3 \u00admediated pathways","volume":"61","author":"Pourhassanali","year":"2016","journal-title":"Reprod. Toxicol"},{"key":"2023112010573774800_CIT0039","doi-asserted-by":"crossref","first-page":"423","DOI":"10.1002\/(SICI)1521-1878(200005)22:5<423::AID-BIES4>3.0.CO;2-0","article-title":"Germ cell suicide: new insights into apoptosis during spermatogenesis","volume":"22","author":"Print","year":"2000","journal-title":"Bioessays"},{"key":"2023112010573774800_CIT0040","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1006\/dbio.2001.0445","article-title":"BCLW mediates survival of postmitotic Sertoli cells by regulating BAX activity","volume":"239","author":"Ross","year":"2001","journal-title":"Dev. Biol"},{"key":"2023112010573774800_CIT0041","doi-asserted-by":"crossref","first-page":"181","DOI":"10.3389\/fendo.2013.00181","article-title":"Paracrine mechanisms involved in the control of early stages of mammalian spermatogenesis","volume":"4","author":"Rossi","year":"2013","journal-title":"Front. Endocrinol"},{"key":"2023112010573774800_CIT0042","doi-asserted-by":"crossref","first-page":"4131","DOI":"10.1091\/mbc.11.12.4131","article-title":"Complex phenotype of mice lacking occludin, a component of tight junction strands","volume":"11","author":"Saitou","year":"2000","journal-title":"Mol. Biol. Cell"},{"key":"2023112010573774800_CIT0043","doi-asserted-by":"crossref","first-page":"769","DOI":"10.1530\/rep.0.1250769","article-title":"Proliferation and functional maturation of Sertoli cells, and their relevance to disorders of testis function in adulthood","volume":"125","author":"Sharpe","year":"2003","journal-title":"Reproduction"},{"key":"2023112010573774800_CIT0044","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1038\/cdd.2017.145","article-title":"Viewing BCL2 and cell death control from an evolutionary perspective","volume":"25","author":"Strasser","year":"2018","journal-title":"Cell Death Differ"},{"key":"2023112010573774800_CIT0045","doi-asserted-by":"crossref","first-page":"406","DOI":"10.1016\/j.ajhg.2009.02.002","article-title":"Mutations in BMP4 are associated with subepithelial, microform, and overt cleft lip","volume":"84","author":"Suzuki","year":"2009","journal-title":"Am. J. Hum. Genet"},{"key":"2023112010573774800_CIT0046","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1016\/S0925-4773(02)00004-7","article-title":"Homeostatic regulation of germinal stem cell proliferation by the GDNF\/FSH pathway","volume":"113","author":"Tadokoro","year":"2002","journal-title":"Mech. Dev"},{"key":"2023112010573774800_CIT0047","doi-asserted-by":"crossref","first-page":"344","DOI":"10.1016\/j.ydbio.2007.09.029","article-title":"Production of knockout mice by gene targeting in multipotent germline stem cells","volume":"312","author":"Takehashi","year":"2007","journal-title":"Dev. Biol"},{"key":"2023112010573774800_CIT0048","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1016\/j.theriogenology.2022.05.015","article-title":"Knockdown of bone morphogenetic protein 4 gene induces apoptosis and inhibits proliferation of bovine cumulus cells","volume":"188","author":"Tian","year":"2022","journal-title":"Theriogenology"},{"key":"2023112010573774800_CIT0049","first-page":"1","article-title":"Loss of occludin expression and impairment of blood-testis barrier permeability in rats with autoimmune orchitis: effect of interleukin 6 on sertoli cell tight junctions","volume":"87","author":"Valeria Perez","year":"2012","journal-title":"Biol. Reprod"},{"key":"2023112010573774800_CIT0050","first-page":"155","article-title":"Cloning and Sequencing of mature fragment of human BMP4 gene","volume":"12","author":"Wang","year":"2000","journal-title":"J. Xi\u2019an Med. Univ"},{"key":"2023112010573774800_CIT0051","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1016\/j.gendis.2014.07.005","article-title":"Bone Morphogenetic Protein (BMP) signaling in development and human diseases.","volume":"1","author":"Wang","year":"2014","journal-title":"Genes Dis"},{"key":"2023112010573774800_CIT0052","doi-asserted-by":"crossref","first-page":"R31","DOI":"10.1530\/REP-15-0546","article-title":"Establishment and applications of male germ cell and Sertoli cell lines","volume":"152","author":"Wang","year":"2016","journal-title":"Reproduction"},{"key":"2023112010573774800_CIT0053","first-page":"1","article-title":"BMP6 regulates Proliferation and apoptosis of human Sertoli cells Via Smad2\/3 and Cyclin D1 pathway and DACH1 and TFAP2A activation","volume":"7","author":"Wang","year":"2017","journal-title":"Sci. Rep"},{"key":"2023112010573774800_CIT0054","doi-asserted-by":"crossref","first-page":"1119","DOI":"10.1242\/dev.012989","article-title":"BMP type I receptor complexes have distinct activities mediating cell fate and axon guidance decisions","volume":"135","author":"Yamauchi","year":"2008","journal-title":"Development"},{"key":"2023112010573774800_CIT0055","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1530\/REP-16-0292","article-title":"BMP4 promotes mouse iPS cell differentiation to male germ cells via Smad1\/5, Gata4, Id1 and Id2","volume":"153","author":"Yang","year":"2017","journal-title":"Reproduction"},{"key":"2023112010573774800_CIT0056","first-page":"77","article-title":"Bone morphogenetic protein 4 inhibits cell proliferation and induces apoptosis in glioma stem cells","volume":"26","author":"Zhou","year":"2011","journal-title":"Cancer Biother. Radiopharm"}],"container-title":["Journal of Animal Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/jas\/advance-article-pdf\/doi\/10.1093\/jas\/skac393\/47368444\/skac393.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jas\/article-pdf\/doi\/10.1093\/jas\/skac393\/53586151\/skac393.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jas\/article-pdf\/doi\/10.1093\/jas\/skac393\/53586151\/skac393.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,20]],"date-time":"2023-11-20T10:58:42Z","timestamp":1700477922000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jas\/article\/doi\/10.1093\/jas\/skac393\/6849478"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,11,28]]},"references-count":56,"URL":"https:\/\/doi.org\/10.1093\/jas\/skac393","relation":{},"ISSN":["0021-8812","1525-3163"],"issn-type":[{"value":"0021-8812","type":"print"},{"value":"1525-3163","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2023,1,1]]},"published":{"date-parts":[[2022,11,28]]},"article-number":"skac393"}}