{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T16:12:03Z","timestamp":1789834323714,"version":"4.0.1"},"reference-count":36,"publisher":"Oxford University Press (OUP)","license":[{"start":{"date-parts":[[2022,12,30]],"date-time":"2022-12-30T00:00:00Z","timestamp":1672358400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/501100022077","name":"Institut national de recherche pour l\u2019agriculture, l\u2019alimentation, et l\u2019environnement","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100022077","id-type":"DOI","asserted-by":"crossref"}]},{"name":"BCF Life Sciences"},{"name":"Convention Industrielle de Formation par la Recherche"},{"DOI":"10.13039\/501100003032","name":"Association Nationale de la Recherche et de la Technologie","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003032","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,1,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Dietary amino acids (AA) supplied as protein or in free form are not only digested and absorbed at different rates but can also induce differences in the intestinal physiology of pigs. We compared the apparent jejunal AA digestibility, intestinal morphology, and gene expression of AA transporters of pigs fed diets providing different forms of AA. Thirty growing pigs (33.7 \u00b1 4.1 kg) were fed one of three experimental diets that provided AA either as protein from feather meal (INT), as free AA and small peptides obtained by extensive acid hydrolysis of feathers (HYD), or as a mix of individual purified AA with the same AA profile as HYD (FAA). Pigs were fed the same quantity of feed, energy, and AA. After 14 d, pigs were slaughtered 3 h after feeding a meal with indigestible markers. Digesta and tissue were collected from different sections of the small intestine. Jejunal digesta was used to measure apparent jejunal digestibility of AA. Samples of the duodenum, jejunum, and ileum were used to measure intestinal morphology and the gene expression of intestinal AA transporters. The measured apparent jejunal digestibility of AA of INT was lower compared to HYD and FAA (P &amp;lt; 0.05). The apparent jejunal digestibility of Cys, Gly, His, Met, and Pro was lower for FAA compared to HYD (P &amp;lt; 0.05). This may be due to the small peptides in HYD, which are absorbed faster than individual AA. The villi area in the ileum of HYD fed pigs was the highest (P &amp;lt; 0.05) among the treatments, which may be associated with the reabsorption of endogenous proteins, which occurs mostly in the ileum. In the duodenum, HYD and FAA had lower expression of PepT1 (P &amp;lt; 0.01) probably due to the rapid transit time of digesta compared to INT fed pigs. Pigs fed HYD expressed more ASCT2 (P = 0.02) and CAT-1 (P = 0.04) in the jejunum compared to the pigs fed the other diets. The expression of these transporters along the intestine depended on the relative abundance of readily absorbable dietary AA. Results showed that dietary AA form can have an influence on the morphology and on the expression of different AA transporters along the different sections of the small intestine.<\/jats:p>","DOI":"10.1093\/jas\/skac417","type":"journal-article","created":{"date-parts":[[2022,12,30]],"date-time":"2022-12-30T16:06:31Z","timestamp":1672416391000},"source":"Crossref","is-referenced-by-count":10,"title":["Apparent jejunal amino acid digestibility, gut morphology, and the expression of intestinal amino acid transporters in pigs fed protein or free amino acids"],"prefix":"10.1093","volume":"101","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8821-2977","authenticated-orcid":false,"given":"Francis Amann","family":"Eugenio","sequence":"first","affiliation":[{"name":"PEGASE, INRAE, Institut Agro , 35590, Saint-Gilles , France"},{"name":"BCF Life Sciences , Boisel, 56140 Pleucadeuc , France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jaap","family":"van Milgen","sequence":"additional","affiliation":[{"name":"PEGASE, INRAE, Institut Agro , 35590, Saint-Gilles , France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jo\u00ebl","family":"Duperray","sequence":"additional","affiliation":[{"name":"BCF Life Sciences , Boisel, 56140 Pleucadeuc , France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Renaud","family":"Sergheraert","sequence":"additional","affiliation":[{"name":"BCF Life Sciences , Boisel, 56140 Pleucadeuc , France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9858-1584","authenticated-orcid":false,"given":"Nathalie","family":"Le Floc\u2019h","sequence":"additional","affiliation":[{"name":"PEGASE, INRAE, Institut Agro , 35590, Saint-Gilles , France"},{"name":"BCF Life Sciences , Boisel, 56140 Pleucadeuc , France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2022,12,30]]},"reference":[{"key":"2023112010571805500_CIT0001","doi-asserted-by":"crossref","first-page":"186","DOI":"10.54846\/jshap\/875","article-title":"Influence of age at weaning and feeding regimes on the postnatal morphology of the porcine small intestine","volume":"23","author":"Al-Masri","year":"2015","journal-title":"J. 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