{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T03:16:32Z","timestamp":1792120592549,"version":"4.4.0"},"reference-count":37,"publisher":"Oxford University Press (OUP)","license":[{"start":{"date-parts":[[2022,12,27]],"date-time":"2022-12-27T00:00:00Z","timestamp":1672099200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31902162"],"award-info":[{"award-number":["31902162"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Project of Science and Technology"},{"DOI":"10.13039\/501100010211","name":"Education Department of Jilin Province","doi-asserted-by":"publisher","award":["JJKH20220361KJ"],"award-info":[{"award-number":["JJKH20220361KJ"]}],"id":[{"id":"10.13039\/501100010211","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,1,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>In vitro-cultured oocytes are separated from the follicular micro-environment in vivo and are more vulnerable than in vivo oocytes to changes in the external environment. This vulnerability disrupts the homeostasis of the intracellular environment, affecting oocyte meiotic completion, and subsequent embryonic developmental competence in vitro. Glycine, one of the main components of glutathione (GSH), plays an important role in the protection of porcine oocytes in vitro. However, the protective mechanism of glycine needs to be further clarified. Our results showed that glycine supplementation promoted cumulus cell expansion and oocyte maturation. Detection of oocyte development ability showed that glycine significantly increased the cleavage rate and blastocyst rate during in vitro fertilization (IVF). SMART-seq revealed that this effect was related to glycine-mediated regulation of cell membrane structure and function. Exogenous addition of glycine significantly increased the levels of the anti-oxidant GSH and the expression of anti-oxidant-related genes (glutathione peroxidase 4 [GPX4], catalase [CAT], superoxide dismutase 1 [SOD1], superoxide dismutase 2 [SOD2], and mitochondrial solute carrier family 25, member 39 [SLC25A39]), decreased the lipid peroxidation caused by reactive oxygen species (ROS) and reduced the level of malondialdehyde (MDA) by enhancing the functions of mitochondria, peroxisomes and lipid droplets (LDs) and the levels of lipid metabolism-related factors (peroxisome proliferator activated receptor coactivator 1 alpha [PGC-1\u03b1], peroxisome proliferator-activated receptor \u03b3 [PPAR\u03b3], sterol regulatory element binding factor 1 [SREBF1], autocrine motility factor receptor [AMFR], and ATP). These effects further reduced ferroptosis and maintained the normal structure and function of the cell membrane. Our results suggest that glycine plays an important role in oocyte maturation and later development by regulating ROS-induced lipid metabolism, thereby protecting against biomembrane damage.<\/jats:p>","DOI":"10.1093\/jas\/skac425","type":"journal-article","created":{"date-parts":[[2022,12,27]],"date-time":"2022-12-27T06:51:37Z","timestamp":1672123897000},"source":"Crossref","is-referenced-by-count":65,"title":["Glycine regulates lipid peroxidation promoting porcine oocyte maturation and early embryonic development"],"prefix":"10.1093","volume":"101","author":[{"given":"Lepeng","family":"Gao","sequence":"first","affiliation":[{"name":"College of Animal Science and Technology, Jilin Agricultural University , Changchun 130118 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chang","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Jilin Agricultural University , Changchun 130118 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yingying","family":"Zheng","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Jilin Agricultural University , Changchun 130118 , China"},{"name":"Ministry of Education Laboratory of Animal Production and Quality Security , Changchun 130118 , China"},{"name":"Jilin Provincial Key Lab of Animal Nutrition and Feed Science , Changchun 130118 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Deyi","family":"Wu","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Jilin Agricultural University , Changchun 130118 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinyuan","family":"Chen","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Jilin Agricultural University , Changchun 130118 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hainan","family":"Lan","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Jilin Agricultural University , Changchun 130118 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Zheng","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Jilin Agricultural University , Changchun 130118 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hao","family":"Wu","sequence":"additional","affiliation":[{"name":"COFCO Corporation , Beijing 100020 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suo","family":"Li","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Jilin Agricultural University , Changchun 130118 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2022,12,27]]},"reference":[{"key":"2023112010571355400_CIT0001","doi-asserted-by":"crossref","first-page":"525","DOI":"10.1089\/ars.2009.2996","article-title":"Peroxisomes are oxidative organelles","volume":"13","author":"Antonenkov","year":"2010","journal-title":"Antioxid. 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