{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,12]],"date-time":"2026-04-12T17:26:37Z","timestamp":1776014797231,"version":"3.50.1"},"reference-count":26,"publisher":"Oxford University Press (OUP)","license":[{"start":{"date-parts":[[2023,1,1]],"date-time":"2023-01-01T00:00:00Z","timestamp":1672531200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,1,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The objective of this work was to determine if specific circulating microRNA (miRNA) differed due to pregnancy status in heifers. Blood samples were collected from heifers 21 d after receiving an in vitro-produced embryo. Pregnancy status was diagnosed 21 d after embryo transfer, equivalent to day 28 of gestation, with rectal ultrasonography. Blood samples from 10 pregnant and 10 nonpregnant heifers were then evaluated for miRNA expression. There were five different miRNAs quantified using delta\u2013delta Ct and qPCR methodology. These miRNAs had previously been associated with early pregnancy in cattle. The miRNA Let-7d-5p was decreased in nonpregnant as compared to pregnant females (P &amp;lt; 0.05). There were no changes in 16-5p, 16-1-3p, 16-2-3p, and 26a-5p associated with pregnancy (P &amp;gt; 0.05). Results demonstrate an opportunity to identify and study the differential expression of miRNAs from the blood of pregnant cows. The Let-7d-5p miRNA is a potential early pregnancy marker and is critical to better understand the early relationships of the cellular and molecular interactions of the cow and embryo.<\/jats:p>","DOI":"10.1093\/jas\/skad054","type":"journal-article","created":{"date-parts":[[2023,2,28]],"date-time":"2023-02-28T05:30:31Z","timestamp":1677562231000},"source":"Crossref","is-referenced-by-count":3,"title":["Short communication: profiling the expression of Let-7d-5p microRNA in circulating blood of pregnant and nonpregnant cows"],"prefix":"10.1093","volume":"101","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6659-4732","authenticated-orcid":false,"given":"Jorge A","family":"De los Santos Funes","sequence":"first","affiliation":[{"name":"Department of Animal and Dairy Sciences, University of Wisconsin Madison , WI 53706-1205 , USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jo\u00e3o Paulo N","family":"Andrade","sequence":"additional","affiliation":[{"name":"Department of Animal and Dairy Sciences, University of Wisconsin Madison , WI 53706-1205 , USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jodi","family":"Berndtson","sequence":"additional","affiliation":[{"name":"Department of Animal and Dairy Sciences, University of Wisconsin Madison , WI 53706-1205 , USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John","family":"Parrish","sequence":"additional","affiliation":[{"name":"Department of Animal and Dairy Sciences, University of Wisconsin Madison , WI 53706-1205 , USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2023,2,28]]},"reference":[{"key":"2023040703590443000_","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1016\/j.cell.2009.01.002","article-title":"MicroRNAs: target recognition and regulatory functions","volume":"136","author":"Bartel","year":"2009","journal-title":"Cell"},{"key":"2023040703590443000_","doi-asserted-by":"crossref","first-page":"2812","DOI":"10.3168\/jds.2016-11588","article-title":"Ability of dairy cows to ensure pregnancy according to breed and genetic merit for production traits under contrasted pasture-based systems","volume":"100","author":"Bedere","year":"2017","journal-title":"J. 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