{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,15]],"date-time":"2026-06-15T10:00:17Z","timestamp":1781517617062,"version":"3.54.1"},"reference-count":33,"publisher":"Oxford University Press (OUP)","license":[{"start":{"date-parts":[[2023,1,1]],"date-time":"2023-01-01T00:00:00Z","timestamp":1672531200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Nature Science Foundation of China","doi-asserted-by":"publisher","award":["32172717"],"award-info":[{"award-number":["32172717"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Nature Science Foundation of China","doi-asserted-by":"publisher","award":["32172756"],"award-info":[{"award-number":["32172756"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Nature Science Foundation of China","doi-asserted-by":"publisher","award":["31972546"],"award-info":[{"award-number":["31972546"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Nature Science Foundation of China","doi-asserted-by":"publisher","award":["32072785"],"award-info":[{"award-number":["32072785"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"JBGS Project of Seed Industry Revitalization in Jiangsu Province","award":["JBGS[2021]030"],"award-info":[{"award-number":["JBGS[2021]030"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,1,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The development of mammalian nonalcoholic fatty liver disease is associated with oxidative stress, reduced mitochondrial function, and increased apoptosis in hepatocytes; however, the expressions of mitochondria-related genes are elevated in goose fatty liver, suggesting that there may be a unique protective mechanism in goose fatty liver. The aim of the study was to investigate this protective mechanism in terms of anti-oxidant capacity. Our data showed no substantial differences in the mRNA expression levels of the apoptosis-related genes including B-cell lymphoma-2 (Bcl-2), BCL2-associated X (Bax), cysteinyl aspartate-specific proteinase-3 (Caspase-3), and cysteinyl aspartate-specific proteinase-9 (Caspase-9) in the livers of the control and overfeeding Lander geese groups. The protein expression levels of Caspase-3 and cleaved Caspase-9 were not markedly different between the groups. Compared with the control group, malondialdehyde content was significantly lower (P &amp;lt; 0.01), glutathione peroxidase (GSH-Px) activity, glutathione (GSH) content, and mitochondrial membrane potential levels were higher (P &amp;lt; 0.01) in the overfeeding group. The mRNA expression levels of the anti-oxidant genes superoxide dismutase 1 (SOD1), glutathione peroxidase 1 (GPX1), and glutathione peroxidase 2 (GPX2) were increased in goose primary hepatocytes after 40 mM and 60 mM glucose treatment. Reactive oxygen species (ROS) levels were significantly reduced (P &amp;lt; 0.01), whereas the mitochondrial membrane potential was maintained at normal levels. The mRNA expression levels of the apoptosis-related genes Bcl-2, Bax, and Caspase-3 were not substantial. There were no significant differences in the expression levels of Caspase-3 and cleaved Caspase-9 proteins. In conclusion, glucose-induced enhanced anti-oxidant capacity may help protect the function of mitochondria and inhibit the occurrence of apoptosis in goose fatty liver.<\/jats:p>","DOI":"10.1093\/jas\/skad059","type":"journal-article","created":{"date-parts":[[2023,2,20]],"date-time":"2023-02-20T19:44:20Z","timestamp":1676922260000},"source":"Crossref","is-referenced-by-count":11,"title":["Glucose-induced enhanced anti-oxidant activity inhibits apoptosis in goose fatty liver"],"prefix":"10.1093","volume":"101","author":[{"given":"Qingyun","family":"Sun","sequence":"first","affiliation":[{"name":"College of Animal Science and Technology, Yangzhou University , Yangzhou, Jiangsu Province 225009 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erpeng","family":"Dai","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Yangzhou University , Yangzhou, Jiangsu Province 225009 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meng","family":"Chen","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Yangzhou University , Yangzhou, Jiangsu Province 225009 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinqi","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Yangzhou University , Yangzhou, Jiangsu Province 225009 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ji\u2019an","family":"Mu","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Yangzhou University , Yangzhou, Jiangsu Province 225009 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Long","family":"Liu","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Yangzhou University , Yangzhou, Jiangsu Province 225009 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tuoyu","family":"Geng","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Yangzhou University , Yangzhou, Jiangsu Province 225009 , China"},{"name":"Joint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education of China, Yangzhou University , Yangzhou, Jiangsu Province 225009 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daoqing","family":"Gong","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Yangzhou University , Yangzhou, Jiangsu Province 225009 , China"},{"name":"Joint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education of China, Yangzhou University , Yangzhou, Jiangsu Province 225009 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanjun","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Mechanical Engineering, Yangzhou University , Yangzhou, Jiangsu Province 225009 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Minmeng","family":"Zhao","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Yangzhou University , Yangzhou, Jiangsu Province 225009 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2023,2,20]]},"reference":[{"key":"2023031905360753400_","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1016\/j.mito.2020.12.010","article-title":"Mitochondrial dysfunction as a mechanistic biomarker in patients with non-alcoholic fatty liver disease (NAFLD)","volume":"57","author":"Ajaz","year":"2021","journal-title":"Mitochondrion"},{"key":"2023031905360753400_","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1586\/egh.11.6","article-title":"Apoptosis in nonalcoholic fatty liver disease: diagnostic and therapeutic implications","volume":"5","author":"Alkhouri","year":"2011","journal-title":"Expert Rev. Gastroenterol. Hepatol"},{"key":"2023031905360753400_","doi-asserted-by":"crossref","first-page":"892","DOI":"10.1152\/japplphysiol.00789.2019","article-title":"Quantification of mitochondrial membrane potential in the isolated rat lung using rhodamine 6G","volume":"128","author":"Audi","year":"2020","journal-title":"J Appl. Physiol. (1985)"},{"key":"2023031905360753400_","doi-asserted-by":"crossref","first-page":"773","DOI":"10.1016\/j.jhep.2010.11.006","article-title":"Drug-induced toxicity on mitochondria and lipid metabolism: mechanistic diversity and deleterious consequences for the liver","volume":"54","author":"Begriche","year":"2011","journal-title":"J. Hepatol"},{"key":"2023031905360753400_","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1007\/s00018-018-2947-0","article-title":"Molecular pathways of nonalcoholic fatty liver disease development and \u00adprogression","volume":"76","author":"Bessone","year":"2019","journal-title":"Cell. Mol. Life Sci."},{"key":"2023031905360753400_","doi-asserted-by":"crossref","first-page":"595","DOI":"10.1016\/j.cld.2004.04.009","article-title":"Mitochondria in nonalcoholic fatty liver disease","volume":"8","author":"Caldwell","year":"2004","journal-title":"Clin. Liver Dis"},{"key":"2023031905360753400_","doi-asserted-by":"crossref","first-page":"813","DOI":"10.1089\/ars.2006.8.813","article-title":"Redox pathways of the mitochondrion","volume":"8","author":"Carla","year":"2006","journal-title":"Antioxid Redox Signal"},{"key":"2023031905360753400_","doi-asserted-by":"crossref","first-page":"582","DOI":"10.1002\/cbin.11137","article-title":"Cell death: a review of the major forms of apoptosis, necrosis and autophagy","volume":"43","author":"D\u2019Arcy","year":"2019","journal-title":"Cell Biol. Int"},{"key":"2023031905360753400_","doi-asserted-by":"crossref","first-page":"268","DOI":"10.1016\/j.jhep.2017.09.003","article-title":"Genetics and epigenetics of NAFLD and NASH: Clinical impact","volume":"68","author":"Eslam","year":"2018","journal-title":"J. Hepatol"},{"key":"2023031905360753400_","doi-asserted-by":"crossref","first-page":"481","DOI":"10.1126\/science.1112125","article-title":"Mitochondrial DNA mutations, oxidative stress, and apoptosis in mammalian aging","volume":"309","author":"G C Kujoth","year":"2005","journal-title":"Science"},{"key":"2023031905360753400_","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1016\/j.bcp.2018.11.020","article-title":"Targeting mitochondria to oppose the progression of nonalcoholic fatty liver disease","volume":"160","author":"Grattagliano","year":"2019","journal-title":"Biochem. Pharmacol"},{"key":"2023031905360753400_","doi-asserted-by":"crossref","first-page":"415","DOI":"10.3390\/antiox10030415","article-title":"Generation of reactive oxygen species by mitochondria","volume":"10","author":"Hernansanz-Agust\u00edn","year":"2021","journal-title":"Antioxidants"},{"key":"2023031905360753400_","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1016\/j.bcp.2014.04.018","article-title":"Cyanidin-3-O-beta-glucoside protects primary mouse hepatocytes against high glucose-induced apoptosis by modulating mitochondrial dysfunction and the PI3K\/Akt pathway","volume":"90","author":"Jiang","year":"2014","journal-title":"Biochem. Pharmacol"},{"key":"2023031905360753400_","doi-asserted-by":"crossref","first-page":"931","DOI":"10.1017\/S0950268818000420","article-title":"Seven cases of probable endotoxin poisoning related to contaminated glutathione infusions","volume":"146","author":"Johnstone","year":"2018","journal-title":"Epidemiol. Infect"},{"key":"2023031905360753400_","doi-asserted-by":"crossref","first-page":"2661","DOI":"10.3748\/wjg.v24.i25.2661","article-title":"Apoptosis and non-alcoholic fatty liver diseases","volume":"24","author":"Kanda","year":"2018","journal-title":"World J. Gastroenterol"},{"key":"2023031905360753400_","doi-asserted-by":"crossref","first-page":"e13622","DOI":"10.1111\/eci.13622","article-title":"Mitochondria, oxidative stress and nonalcoholic fatty liver disease: a complex relationship","volume":"52","author":"Karkucinska-Wieckowska","year":"2022","journal-title":"Eur. J. Clin. Invest"},{"key":"2023031905360753400_","first-page":"603","article-title":"Anti-obesity effects of Diospyros lotus leaf extract in mice with high-fat diet-induced obesity","volume":"43","author":"Kim","year":"2019","journal-title":"Int. J. Mol. Med"},{"key":"2023031905360753400_","doi-asserted-by":"crossref","first-page":"3991","DOI":"10.4238\/2014.May.23.10","article-title":"Roles of Fas\/Fasl, Bcl-2\/Bax, and Caspase-8 in rat nonalcoholic fatty liver disease pathogenesis","volume":"13","author":"Li","year":"2014","journal-title":"Genet. Mol. Res"},{"key":"2023031905360753400_","doi-asserted-by":"crossref","first-page":"428","DOI":"10.1002\/tox.21769","article-title":"The roles of endoplasmic reticulum stress and mitochondrial apoptotic signaling pathway in quercetin-mediated cell death of human prostate cancer PC-3 cells","volume":"29","author":"Liu","year":"2014","journal-title":"Environ. Toxicol"},{"key":"2023031905360753400_","doi-asserted-by":"crossref","DOI":"10.1186\/s12917-018-1653-3","article-title":"Role of miR29c in goose fatty liver is mediated by its target genes that are involved in energy homeostasis and cell growth","volume":"14","author":"Liu","year":"2018","journal-title":"BMC Vet. Res"},{"key":"2023031905360753400_","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1007\/s11010-016-2737-7","article-title":"Fads1 and 2 are promoted to meet instant need for long-chain polyunsaturated fatty acids in goose fatty liver","volume":"418","author":"Osman","year":"2016","journal-title":"Mol. Cell. Biochem"},{"key":"2023031905360753400_","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1016\/j.cbpb.2015.11.006","article-title":"Expression of mitochondria-related genes is elevated in overfeeding-induced goose fatty liver","volume":"192","author":"Osman","year":"2016","journal-title":"Comp Biochem Physiol B Biochem Mol Biol"},{"key":"2023031905360753400_","doi-asserted-by":"crossref","first-page":"14205","DOI":"10.3748\/wjg.v20.i39.14205","article-title":"Oxidative stress, cardiolipin and mitochondrial dysfunction in \u00adnonalcoholic fatty liver disease","volume":"20","author":"Paradies","year":"2014","journal-title":"World J. Gastroenterol"},{"key":"2023031905360753400_","doi-asserted-by":"crossref","first-page":"1977","DOI":"10.1194\/jlr.M085613","article-title":"Mitochondrial dysfunction-related lipid changes occur in nonalcoholic fatty liver disease progression","volume":"59","author":"Peng","year":"2018","journal-title":"J. Lipid Res"},{"key":"2023031905360753400_","doi-asserted-by":"crossref","first-page":"723784","DOI":"10.3389\/fphar.2021.723784","article-title":"Ginsenoside Rb1 attenuates triptolide-induced cytotoxicity in HL-7702 cells via the activation of keap1\/Nrf2\/ARE pathway","volume":"12","author":"Peng","year":"2021","journal-title":"Front. Pharmacol"},{"key":"2023031905360753400_","doi-asserted-by":"crossref","first-page":"119905","DOI":"10.1016\/j.lfs.2021.119905","article-title":"Pancreastatin induces hepatic steatosis in type 2 diabetes by impeding mitochondrial functioning","volume":"284","author":"Reza","year":"2021","journal-title":"Life Sci"},{"key":"2023031905360753400_","doi-asserted-by":"crossref","first-page":"377","DOI":"10.1038\/s41575-019-0144-8","article-title":"Past, present and future perspectives in nonalcoholic fatty liver disease","volume":"16","author":"Sanyal","year":"2019","journal-title":"Nat. Rev. Gastroenterol. Hepatol"},{"key":"2023031905360753400_","doi-asserted-by":"crossref","first-page":"1101","DOI":"10.1038\/nprot.2008.73","article-title":"Analyzing real-time PCR data by the comparative C(T) method","volume":"3","author":"Schmittgen","year":"2008","journal-title":"Nat. Protocols"},{"key":"2023031905360753400_","doi-asserted-by":"crossref","first-page":"2539","DOI":"10.1093\/nar\/gkp100","article-title":"Oxidative stress induces degradation of mitochondrial DNA","volume":"37","author":"Shokolenko","year":"2009","journal-title":"Nucleic Acids Res"},{"key":"2023031905360753400_","doi-asserted-by":"crossref","first-page":"1700","DOI":"10.1002\/tox.22172","article-title":"Endotoxin-induced acute lung injury in mice is protected by 5,7-dihydroxy-8-methoxyflavone via inhibition of oxidative stress and HIF-1alpha","volume":"31","author":"Sun","year":"2016","journal-title":"Environ. Toxicol"},{"key":"2023031905360753400_","doi-asserted-by":"crossref","first-page":"730","DOI":"10.1093\/abbs\/gmv061","article-title":"Mitochondrial fusion\/fission process involved in the improvement of catalpol on high glucose-induced hepatic mitochondrial dysfunction","volume":"47","author":"Xu","year":"2015","journal-title":"Acta Biochim. Biophys. Sin"},{"key":"2023031905360753400_","doi-asserted-by":"crossref","first-page":"2108","DOI":"10.1016\/j.foodchem.2013.05.019","article-title":"Effects of flavonoids from Rosa laevigata Michx fruit against high-fat diet-induced non-alcoholic fatty liver disease in rats","volume":"141","author":"Zhang","year":"2013","journal-title":"Food Chem"},{"key":"2023031905360753400_","doi-asserted-by":"crossref","first-page":"909","DOI":"10.1152\/physrev.00026.2013","article-title":"Mitochondrial reactive oxygen species (ROS) and ROS-induced ROS release","volume":"94","author":"Zorov","year":"2014","journal-title":"Physiol. Rev"}],"container-title":["Journal of Animal Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/jas\/advance-article-pdf\/doi\/10.1093\/jas\/skad059\/49280769\/skad059.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jas\/article-pdf\/doi\/10.1093\/jas\/skad059\/49559666\/skad059.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jas\/article-pdf\/doi\/10.1093\/jas\/skad059\/49559666\/skad059.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,3,25]],"date-time":"2023-03-25T21:43:26Z","timestamp":1679780606000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jas\/article\/doi\/10.1093\/jas\/skad059\/7048970"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,1,1]]},"references-count":33,"URL":"https:\/\/doi.org\/10.1093\/jas\/skad059","relation":{},"ISSN":["0021-8812","1525-3163"],"issn-type":[{"value":"0021-8812","type":"print"},{"value":"1525-3163","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2023,1,1]]},"published":{"date-parts":[[2023,1,1]]},"article-number":"skad059"}}