{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T15:55:34Z","timestamp":1791647734356,"version":"4.3.4"},"reference-count":45,"publisher":"Oxford University Press (OUP)","funder":[{"name":"Nonassistance Cooperative Agreement","award":["5042-32630-004-001-S"],"award-info":[{"award-number":["5042-32630-004-001-S"]}]},{"name":"USDA-ARS Forage-Animal Production Research Unit"},{"DOI":"10.13039\/100011136","name":"University of Kentucky Agricultural Experiment Station","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100011136","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,1,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Ergovaline (ERV), produced in toxic endophyte-infected tall fescue, causes potent vasoconstriction of bovine peripheral and visceral vasculature. Ergovaline acts as both an agonist and an antagonist in bovine gut blood vessels through serotonin (5-HT) receptors and it appears that the type of action could be influenced by the extent of ERV exposure. Because it was unclear how the duration of ERV exposure influences 5-HT-mediated vasoactivity, experiments were designed to evaluate how simultaneous or prior ERV exposure influenced 5-HT-mediated vasoactivity of mesenteric artery (MA) and vein (MV) segments from Holstein steers (N = 10). Vessels were incubated in Krebs\u2013Henseleit buffer containing 0, 0.01, or 0.1 \u03bcM ERV for 24 h prior to the 5-HT dose-response or exposed to fixed concentrations of 0, 0.01, or 0.1 \u03bcM ERV simultaneously during the 5-HT dose-response. Vessels were suspended in chambers of a multimyograph containing Krebs\u2013Henseleit buffer and equilibrated to 1 g tension for 90 min. Vessels were exposed to increasing concentrations of 5-HT (5 \u00d7 10\u22128 M to 1 \u00d7 10\u22124 M) every 15 min and contractile responses were normalized as a percentage of the maximum contractile response induced by 120 mM KCl reference addition. Two-way analysis of variance was used to separately analyze data for each vessel type and duration of exposure using the MIXED procedure of SAS. When 5-HT concentration increased from 5 \u00d7 10\u22128 to 1 \u00d7 10\u22126 M, simultaneous addition of 0.1 \u03bcM ERV increased (P &amp;lt; 0.01) the contractile response of MV compared with additions of 0 and 0.01 \u03bcM ERV. At 1 \u00d7 10\u22124 M 5-HT, the simultaneous presence of 0.01 and 0.1 \u03bcM ERV decreased (P &amp;lt; 0.01) the contractile response of both MA and MV compared with 0 \u03bcM ERV addition. As 5-HT concentrations increased, the contractile response increased (P &amp;lt; 0.01) in both MA and MV with no previous ERV exposure, but decreased in MA and MV with 24 h prior exposure to 0.01 and 0.1 \u03bcM ERV. These data demonstrate that the duration of ERV exposure influences 5-HT-mediated vasoconstriction and likely vasorelaxation in bovine mesenteric vasculature. If ERV and 5-HT exposure occur simultaneously, ERV can act as a partial agonist of 5-HT-mediated vasoconstriction. If 5-HT exposure occurs after blood vessels have had prior ERV exposure, it appears that 5-HT may induce vasorelaxation of blood vessels. More research is needed to identify cellular and molecular mechanisms involved with 5-HT-mediated vasoactivity.<\/jats:p>","DOI":"10.1093\/jas\/skad100","type":"journal-article","created":{"date-parts":[[2023,4,2]],"date-time":"2023-04-02T17:01:18Z","timestamp":1680454878000},"source":"Crossref","is-referenced-by-count":5,"title":["Duration of ergovaline exposure influences serotonin-mediated vasoactivity of bovine mesenteric vasculature"],"prefix":"10.1093","volume":"101","author":[{"given":"Ronald J","family":"Trotta","sequence":"first","affiliation":[{"name":"Department of Animal and Food Sciences, University of Kentucky , Lexington, KY 40546 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5187-1920","authenticated-orcid":false,"given":"David L","family":"Harmon","sequence":"additional","affiliation":[{"name":"Department of Animal and Food Sciences, University of Kentucky , Lexington, KY 40546 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huihua","family":"Ji","sequence":"additional","affiliation":[{"name":"Kentucky Tobacco Research and Development Center, University of Kentucky , Lexington, KY 40546 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9728-1889","authenticated-orcid":false,"given":"James L","family":"Klotz","sequence":"additional","affiliation":[{"name":"USDA-ARS, Forage-Animal Production Research Unit , Lexington , KY 40546 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2023,4,2]]},"reference":[{"key":"2023042620005279600_CIT0001","doi-asserted-by":"crossref","first-page":"744","DOI":"10.3390\/toxins12120744","article-title":"Rumen and serum metabolomes in response to endophyte-infected tall fescue seed and isoflavone supplementation in beef steers","volume":"12","author":"Ault-Seay","year":"2020","journal-title":"Toxins"},{"key":"2023042620005279600_CIT0002","first-page":"3","article-title":"Ergot compounds: a synopsis, ergot compounds and brain function: neuroendocrine and neuropsychiatric aspects no","author":"Berde","year":"1980"},{"key":"2023042620005279600_CIT0003","doi-asserted-by":"crossref","first-page":"941","DOI":"10.2135\/cropsci1982.0011183X002200050010x","article-title":"Association of N-acetyl loline and N-formyl loline with Epichloe typhina in tall fescue","volume":"22","author":"Bush","year":"1982","journal-title":"Crop Sci"},{"key":"2023042620005279600_CIT0004","doi-asserted-by":"crossref","first-page":"36","DOI":"10.3389\/fnins.2013.00036","article-title":"Serotonin and the regulation of mammalian energy balance","volume":"7","author":"Donovan","year":"2013","journal-title":"Front. 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