{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T22:24:37Z","timestamp":1792794277769,"version":"4.5.0"},"reference-count":61,"publisher":"Oxford University Press (OUP)","license":[{"start":{"date-parts":[[2023,6,27]],"date-time":"2023-06-27T00:00:00Z","timestamp":1687824000000},"content-version":"vor","delay-in-days":177,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32072718"],"award-info":[{"award-number":["32072718"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key R&D Program of China","doi-asserted-by":"publisher","award":["2021YFD1200903"],"award-info":[{"award-number":["2021YFD1200903"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key R&D Program of China","doi-asserted-by":"publisher","award":["2021YFD1200900"],"award-info":[{"award-number":["2021YFD1200900"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010038","name":"Earmarked Fund for China Agriculture Research System","doi-asserted-by":"publisher","award":["CARS-36"],"award-info":[{"award-number":["CARS-36"]}],"id":[{"id":"10.13039\/501100010038","id-type":"DOI","asserted-by":"publisher"}]},{"name":"China Agriculture Research System","id":[{"id":"https:\/\/ror.org\/03p5eky76","id-type":"ROR","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100005045","name":"MOF","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100005045","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100009233","name":"MARA","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100009233","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Seed Fund"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,1,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Considering that artificial insemination is the most widely used assisted reproductive technique in the dairy industry, the semen quality of bulls is very important for selecting excellent stud bulls. Sperm motility is one of the important traits of semen quality, and related genes may be regulated by environmental factors. Seminal plasma can affect sperm cell transcriptome and further affect sperm motility through exosome or other processes. However, the molecular regulation mechanism of bull sperm motility has not been studied by combining the sperm cell transcriptome with seminal plasma metabolome. The number of motile sperm per ejaculate (NMSPE) is an integrated indicator for assessing sperm motility in stud bulls. In the present study, we selected 7 bulls with higher NMSPE (5,698.55 million +\/\u2212 945.40 million) as group H and 7 bulls with lower NMSPE (2,279.76 million +\/\u2212 1,305.69 million) as group L from 53 Holstein stud bulls. The differentially expressed genes (DEGs) in sperm cells were evaluated between the two groups (H vs. L). We conducted gene co-expression network analysis (WGCNA) on H and L groups of bulls, as well as two monozygotic twin Holstein bulls with different NMSPE values, to screen candidate genes for NMSPE. The regulatory effect of seminal plasma metabolome on the candidate genes of NMSPE was also investigated. A total of 1,099 DEGs were identified in the sperm cells of H and L groups. These DEGs were primarily concentrated in energy metabolism and sperm cell transcription. The significantly enriched Kyoto encyclopedia of genes and genomes (KEGG) pathways of the 57 differential metabolites were the aminoacyl\u2013tRNA biosynthesis pathway and vitamin B6 metabolism pathway. Our study discovered 14 genes as the potential candidate markers for sperm motility, including FBXO39. We observed a broad correlation between transcriptome of sperm cells and seminal plasma metabolome, such as three metabolites, namely, mesaconic acid, 2-coumaric acid, and 4-formylaminoantipyrine, might regulate FBXO39 expression through potential pathways. The genes related to seminal plasma metabolites expressed in sperm cells are not only located near the quantitative trait loci of reproductive traits, but also enriched in the genome-wide association study signal of sire conception rate. Collectively, this study was the first to investigate the interplays among transcriptome of sperm cells and seminal plasma metabolome from Holstein stud bulls with different sperm motility.<\/jats:p>","DOI":"10.1093\/jas\/skad214","type":"journal-article","created":{"date-parts":[[2023,6,27]],"date-time":"2023-06-27T05:09:31Z","timestamp":1687842571000},"source":"Crossref","is-referenced-by-count":17,"title":["Integrating sperm cell transcriptome and seminal plasma metabolome to analyze the molecular regulatory mechanism of sperm motility in Holstein stud bulls"],"prefix":"10.1093","volume":"101","author":[{"given":"Wenlong","family":"Li","sequence":"first","affiliation":[{"name":"Key Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Agriculture and Rural Affairs & National Engineering Laboratory for Animal Breeding, Department of Animal Breeding and Genetics, College of Animal Science and Technology, China Agricultural University , Beijing 100193 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siyuan","family":"Mi","sequence":"additional","affiliation":[{"name":"Key Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Agriculture and Rural Affairs & National Engineering Laboratory for Animal Breeding, Department of Animal Breeding and Genetics, College of Animal Science and Technology, China Agricultural University , Beijing 100193 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinning","family":"Zhang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Agriculture and Rural Affairs & National Engineering Laboratory for Animal Breeding, Department of Animal Breeding and Genetics, College of Animal Science and Technology, China Agricultural University , Beijing 100193 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xueqin","family":"Liu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Agriculture and Rural Affairs & National Engineering Laboratory for Animal Breeding, Department of Animal Breeding and Genetics, College of Animal Science and Technology, China Agricultural University , Beijing 100193 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siqian","family":"Chen","sequence":"additional","affiliation":[{"name":"Key Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Agriculture and Rural Affairs & National Engineering Laboratory for Animal Breeding, Department of Animal Breeding and Genetics, College of Animal Science and Technology, China Agricultural University , Beijing 100193 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuli","family":"Liu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Agriculture and Rural Affairs & National Engineering Laboratory for Animal Breeding, Department of Animal Breeding and Genetics, College of Animal Science and Technology, China Agricultural University , Beijing 100193 , China"},{"name":"School of Life Sciences, Westlake University , Hangzhou, Zhejiang 310024 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xia","family":"Feng","sequence":"additional","affiliation":[{"name":"Key Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Agriculture and Rural Affairs & National Engineering Laboratory for Animal Breeding, Department of Animal Breeding and Genetics, College of Animal Science and Technology, China Agricultural University , Beijing 100193 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongjie","family":"Tang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Agriculture and Rural Affairs & National Engineering Laboratory for Animal Breeding, Department of Animal Breeding and Genetics, College of Animal Science and Technology, China Agricultural University , Beijing 100193 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanhua","family":"Li","sequence":"additional","affiliation":[{"name":"Beijing Dairy Cattle Center , Qinghe\u2019nanzhen Deshengmenwai Road, Beijing 100192 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lin","family":"Liu","sequence":"additional","affiliation":[{"name":"Beijing Dairy Cattle Center , Qinghe\u2019nanzhen Deshengmenwai Road, Beijing 100192 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lingzhao","family":"Fang","sequence":"additional","affiliation":[{"name":"MRC Human Genetics Unit, Institute of Genetics and Molecular Medicine, University of Edinburgh , Edinburgh EH4 2XU , UK"},{"name":"Center for Quantitative Genetics and Genomics (QGG), Aarhus University , Aarhus , Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shengli","family":"Zhang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Agriculture and Rural Affairs & National Engineering Laboratory for Animal Breeding, Department of Animal Breeding and Genetics, College of Animal Science and Technology, China Agricultural University , Beijing 100193 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Yu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Agriculture and Rural Affairs & National Engineering Laboratory for Animal Breeding, Department of Animal Breeding and Genetics, College of Animal Science and Technology, China Agricultural University , Beijing 100193 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2023,6,27]]},"reference":[{"key":"2023071917144202100_CIT0001","first-page":"e13556","article-title":"Seminal plasma vitamin B-6 levels in men with asthenozoospermia and men with normal sperm motility, a measurement using liquid chromatography with tandem mass spectrometry","volume":"5","author":"Banihani","year":"2020","journal-title":"Andrologia"},{"key":"2023071917144202100_CIT0002","first-page":"274","article-title":"Factors affecting breeding soundness classification of beef bulls examined at the Western College of Veterinary Medicine","volume":"4","author":"Barth","year":"2002","journal-title":"Can. 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