{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,2]],"date-time":"2025-12-02T18:44:21Z","timestamp":1764701061549,"version":"3.41.2"},"reference-count":52,"publisher":"Oxford University Press (OUP)","license":[{"start":{"date-parts":[[2023,10,4]],"date-time":"2023-10-04T00:00:00Z","timestamp":1696377600000},"content-version":"vor","delay-in-days":276,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Michigan AgBio Research, Michigan State University","award":["MICL02732"],"award-info":[{"award-number":["MICL02732"]}]},{"name":"Multistate Research Project NC-1184","award":["MICL04228"],"award-info":[{"award-number":["MICL04228"]}]},{"name":"Beef Nutrition Program at South Dakota State University"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,1,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Our investigation elucidated the effects of severe temperature fluctuations on cellular and physiological responses in beef cattle. Eighteen Red Angus beef steers with an average body weight of 351\u2005\u00b1\u200524.5 kg were divided into three treatment groups: 1) Control (CON), exposed to a temperature-humidity index (THI) of 42 for 6 h without any temperature changes; 2) Transport (TP), subjected to a one-mile trailer trip with a THI of 42 for 6 h; and 3) Temperature swing (TS), exposed to a one-mile trailer trip with a THI shift from 42 to 72\u201375 for 3 h. Our findings indicate that TS can induce thermal stress in cattle, regardless of whether the overall temperature level is excessively high or not. Behavioral indications of extreme heat stress in the cattle were observed, including extended tongue protrusion, reduced appetite, excessive salivation, and increased respiratory rate. Furthermore, we observed a pronounced overexpression (P\u2005&amp;lt;\u20050.05) of heat shock proteins (HSPs) 20, 27, and 90 in response to the TS treatment in the longissimus muscle (LM). Alterations in signaling pathways associated with skeletal muscle growth were noted, including the upregulation (P\u2005&amp;lt;\u20050.01) of Pax7, Myf5, and myosin heavy chain (MHC) isoforms. In addition, an increase (P\u2005&amp;lt;\u20050.05) in transcription factors associated with adipogenesis was detected (P\u2005&amp;lt;\u20050.05), such as PPAR\u03b3, C\/EBP\u03b1, FAS, and SCD in the TS group, suggesting the potential for adipose tissue accumulation due to temperature fluctuations. Our data illustrated the potential impacts of these temperature fluctuations on the growth of skeletal muscle and adipose tissue in beef cattle.<\/jats:p>","DOI":"10.1093\/jas\/skad343","type":"journal-article","created":{"date-parts":[[2023,10,4]],"date-time":"2023-10-04T14:30:06Z","timestamp":1696429806000},"source":"Crossref","is-referenced-by-count":3,"title":["Temperature Fluctuations Modulate Molecular Mechanisms in Skeletal Muscle and Influence Growth Potential in Beef Steers"],"prefix":"10.1093","volume":"101","author":[{"given":"Zachary K","family":"Smith","sequence":"first","affiliation":[{"name":"Department of Animal Science, South Dakota State University , Brookings, SD , USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Erika","family":"Eckhardt","sequence":"additional","affiliation":[{"name":"Department of Animal Science, Michigan State University , East Lansing, MI , USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Won Seob","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Animal Science, Michigan State University , East Lansing, MI , USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana Clara Baio","family":"Menezes","sequence":"additional","affiliation":[{"name":"Department of Animal Science, South Dakota State University , Brookings, SD , USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Warren C","family":"Rusche","sequence":"additional","affiliation":[{"name":"Department of Animal Science, South Dakota State University , Brookings, SD , USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jongkyoo","family":"Kim","sequence":"additional","affiliation":[{"name":"Animal Science and Food Science and Human Nutrition, Michigan State University , East Lansing, MI , USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2023,10,4]]},"reference":[{"key":"2023101816391461600_CIT0001","doi-asserted-by":"crossref","first-page":"399","DOI":"10.1016\/S0248-4900(03)00087-X","article-title":"Myosin heavy chain isoforms in postnatal muscle development of mice","volume":"95","author":"Agbulut","year":"2003","journal-title":"Biol. 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