{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T02:29:48Z","timestamp":1789180188413,"version":"build-2803163510"},"reference-count":36,"publisher":"Oxford University Press (OUP)","license":[{"start":{"date-parts":[[2023,1,1]],"date-time":"2023-01-01T00:00:00Z","timestamp":1672531200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,1,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Enterotoxigenic Escherichia coli (ETEC) causes post-weaning diarrhea in piglets, significantly impacting animal welfare and production efficiency. The two primary ETEC pathotypes associated with post-weaning diarrhea are ETEC F4 and ETEC F18. During the post-weaning period, piglets may be exposed to both ETEC F4 and ETEC F18. However, the effects of coinfection by both strains have not been studied. Short chain fatty acid feed additives, such as butyrate and valerate, are being investigated for their potential to improve animal performance and disease resistance. Therefore, this pilot experiment aimed to test the effects of butyrate glycerides or valerate glycerides on growth performance, diarrhea incidence, and immune responses of piglets under ETEC F4-ETEC F18 coinfection conditions. Twenty piglets were individually housed and assigned to one of the three dietary treatments immediately at weaning (21 to 24 d of age). The dietary treatments included control (basal diet formulation), control supplemented with 0.1% butyrate glycerides or 0.1% valerate glycerides. After a 7-d adaptation, all pigs were inoculated with ETEC F4 and ETEC F18 (0.5\u2005\u00d7\u2005109 CFU\/1.5 mL dose for each strain) on three consecutive days. Pigs and feeders were weighed throughout the trial to measure growth performance. Fecal cultures were monitored for hemolytic coliforms, and blood samples were collected for whole blood and serum analysis. Pigs fed valerate glycerides tended (P\u2005=\u20050.095) to have higher final body weight compared with control. The overall severity of diarrhea was significantly (P\u2005&amp;lt;\u20050.05) lower in both treatment groups than control. Pigs fed valerate glycerides tended (P\u2005=\u20050.061) to have lower neutrophils and had significantly (P\u2005&amp;lt;\u20050.05) lower serum TNF-\u03b1 on day 4 post-inoculation. This pilot experiment established an appropriate experimental dose for an ETEC F4-ETEC F18 coinfection disease model in weaned piglets. Results also suggest that butyrate glycerides and valerate glycerides alleviated diarrhea and regulated immune responses in piglets coinfected with ETEC F4 and ETEC F18.<\/jats:p>","DOI":"10.1093\/jas\/skad401","type":"journal-article","created":{"date-parts":[[2023,12,4]],"date-time":"2023-12-04T07:31:22Z","timestamp":1701675082000},"source":"Crossref","is-referenced-by-count":16,"title":["Dietary butyrate and valerate glycerides impact diarrhea severity and immune response of weaned piglets under ETEC F4-ETEC F18 coinfection conditions"],"prefix":"10.1093","volume":"101","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2689-6197","authenticated-orcid":false,"given":"Lauren","family":"Kovanda","sequence":"first","affiliation":[{"name":"Department of Animal Science, University of California, Davis , Davis, CA 95616 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jungjae","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Nutrition, University of California, Davis , Davis, CA 95616 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sangwoo","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Animal Science, University of California, Davis , Davis, CA 95616 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5854-6047","authenticated-orcid":false,"given":"Kwangwook","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Animal Science, Michigan State University , East Lansing, MI 48824 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xunde","family":"Li","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine, University of California, Davis , Davis, CA 95616 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanhong","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Animal Science, University of California, Davis , Davis, CA 95616 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2023,12,4]]},"reference":[{"key":"2023121501425513900_CIT0001","first-page":"73","article-title":"Presentation of postweaning Escherichia coli diarrhea in southern Ontario, prevalence of hemolytic E coli serogroups involved, and their antimicrobial resistance patterns","volume":"66","author":"Amezcua","year":"2002","journal-title":"Can. 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