{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,2]],"date-time":"2026-04-02T19:19:20Z","timestamp":1775157560637,"version":"3.50.1"},"reference-count":39,"publisher":"Oxford University Press (OUP)","license":[{"start":{"date-parts":[[2023,1,1]],"date-time":"2023-01-01T00:00:00Z","timestamp":1672531200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,1,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>The effects of a novel direct-fed microbial (DFM) on feedlot performance, carcass characteristics, digestibility, ruminal morphology, and volatile fatty acid (VFA) profile of finishing steers were evaluated. Single-source Angus-crossbred yearling steers (n\u2005=\u2005144; initial body weight (BW)\u2005=\u2005371\u2005\u00b1\u200519\u00a0kg) were used in a randomized complete block design. Steers were blocked by initial BW and randomly assigned to treatments (12 pens\/treatment; 4 steers\/pen). Treatments included (A) CONTROL (no DFM, tylosin, or monensin, (B) MONTY (monensin sodium [330\u00a0mg\/animal-daily] and tylosin phosphate [90\u00a0mg\/animal-daily]), and (C) MONPRO (monensin sodium [same as previous] and Lactobacillus salivarius L28 [1\u2005\u00d7\u2005106 CFU\/animal-daily]). Treatments were included in a steam-flaked corn-based finisher diet offered once daily using a clean-bunk management for ~149\u00a0d. The digestibility assessment was performed from days 70 to 74. Ruminal fluid and rumen tissue samples were collected at the slaughter for VFA profile and papillae morphology analyses, respectively. Data were analyzed using the GLIMMIX procedure of SAS with pen serving as the experimental unit, treatment as fixed effect, and BW block as random effect. Steers offered MONPRO had on average 5.3% less (P\u2005&amp;lt;\u20050.01) dry matter intake (9.56\u00a0kg\/d) compared with either CONTROL (10.16\u00a0kg\/d) or MONTY (9.96\u00a0kg\/d). The carcass-adjusted final BW (613\u00a0kg; P\u2005=\u20050.23), overall average daily gain (1.64\u00a0kg\/d; P\u2005=\u20050.23), and gain-efficiency (0.165; P\u2005=\u20050.61) were not affected by treatments. Steers offered CONTROL had greater (P\u2005&amp;lt;\u20050.01) marbling score and tended (P\u2005=\u20050.06) to have less carcasses grading Select and tended (P\u2005=\u20050.10) to have more carcasses grading Upper-Choice, while other carcass characteristics and liver-abscesses were not affected (P\u2005\u2265\u20050.23) by treatments. The digestibility of nutrients (P\u2005\u2265\u20050.13) and the ruminal VFA profile (P\u2005\u2265\u20050.12) were not affected by treatments. Steers offered MONPRO tended (P\u2005=\u20050.09) to have 16% greater average papillae number compared to other treatments. Yearlings offered finishing diets containing L. salivarius L28 plus monensin did not affect growth performance, digestibility, or ruminal VFA, but reduced feed intake. Carcass quality was negatively affected by treatments, while animals consuming L. salivarius L28 and monensin tended to improve ruminal morphology. Current findings in ruminal morphology and feed intake may warrant further assessment of diets containing L. salivarius L28 on beef cattle food safety aspects.<\/jats:p>","DOI":"10.1093\/jas\/skad404","type":"journal-article","created":{"date-parts":[[2023,12,9]],"date-time":"2023-12-09T10:54:45Z","timestamp":1702119285000},"source":"Crossref","is-referenced-by-count":6,"title":["Effects of a novel direct-fed microbial on growth performance, carcass characteristics, nutrient digestibility, and ruminal morphology of beef feedlot steers"],"prefix":"10.1093","volume":"101","author":[{"given":"Tosha S","family":"Opheim","sequence":"first","affiliation":[{"name":"Department of Animal and Food Sciences, Texas Tech University , Lubbock, TX 79409 , USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3791-2308","authenticated-orcid":false,"given":"Jhones O","family":"Sarturi","sequence":"additional","affiliation":[{"name":"Department of Animal and Food Sciences, Texas Tech University , Lubbock, TX 79409 , USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Barbara M","family":"Rodrigues","sequence":"additional","affiliation":[{"name":"Department of Animal and Food Sciences, Texas Tech University , Lubbock, TX 79409 , USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kendra\u00a0K","family":"Nightingale","sequence":"additional","affiliation":[{"name":"Department of Animal and Food Sciences, Texas Tech University , Lubbock, TX 79409 , USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mindy","family":"Brashears","sequence":"additional","affiliation":[{"name":"Department of Animal and Food Sciences, Texas Tech University , Lubbock, TX 79409 , USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Beatriz Q","family":"Reis","sequence":"additional","affiliation":[{"name":"Department of Animal and Food Sciences, Texas Tech University , Lubbock, TX 79409 , USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael A","family":"Ballou","sequence":"additional","affiliation":[{"name":"Department of Veterinary Sciences, Texas Tech University , Lubbock, TX 79409 , USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Markus","family":"Miller","sequence":"additional","affiliation":[{"name":"Department of Animal and Food Sciences, Texas Tech University , Lubbock, TX 79409 , USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Diego E","family":"Casas","sequence":"additional","affiliation":[{"name":"Department of Animal and Food Sciences, Texas Tech University , Lubbock, TX 79409 , USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2023,12,9]]},"reference":[{"key":"2024010216312928200_CIT0001","volume-title":"Official method of analysis","author":"AOAC","year":"2005","edition":"18th ed"},{"key":"2024010216312928200_CIT0002","doi-asserted-by":"crossref","first-page":"e00955-17","DOI":"10.1128\/genomeA.00955-17","article-title":"Draft genome sequence of Lactobacillus salivarius L28 isolated from ground beef","volume":"5","author":"Ayala","year":"2017","journal-title":"Genome Announc"},{"key":"2024010216312928200_CIT0003","doi-asserted-by":"crossref","first-page":"146","DOI":"10.1016\/j.anifeedsci.2006.09.011","article-title":"Daily rumen pH pattern of loose-housed dairy cattle as affected by feeding pattern and live yeast supplementation","volume":"136","author":"Bach","year":"2007","journal-title":"Anim. 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