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No studies have reported bioinformatic analyses of X- and Y-bearing sperm of dairy goats, although this will contribute to exploring novel and applied sex-skewing technologies. Regulatory subunit of the histone methyltransferase complex (DPY30) was determined to be\u00a0the key differentially expressed protein (DEP) among 15 DEPs identified in the present study. The spatiotemporal expression of DPY30 strongly suggested a functional involvement of the protein in spermatogenesis. DPY30 promoted meiosis via upregulating SYCP3, which played a crucial role in mediating sex ratio skewing in goats. Although DPY30 suppressed the self-renewal of spermatogonia stem cells through AKT\/PLZF, DPY30 inhibition in the testis did not induce testicular dysgenesis. Based on the biosafety assessment in mice testes, lentivirus-mediated DPY30 knockdown in bucks\u2019 testes increased X-bearing sperm proportion and female kids\u2019 rate (22.8 percentage points) without affecting sperm quality, pregnancy rate, and kidding rate. This study provides the first evidence of the DEGs in the sexed sperm of dairy goats. DPY30 inhibition in the testes of bucks increased the female kids\u2019 rate without influencing reproductive performance. The present study provides evidence for expanding the female dairy goat population to address the concern of dairy product shortage.<\/jats:p>","DOI":"10.1093\/jas\/skad422","type":"journal-article","created":{"date-parts":[[2024,1,2]],"date-time":"2024-01-02T05:44:02Z","timestamp":1704174242000},"source":"Crossref","is-referenced-by-count":0,"title":["A novel regulatory sex-skewing method that inhibits testicular DPY30 expression to increase female rate of dairy goat offspring"],"prefix":"10.1093","volume":"102","author":[{"given":"Huanshan","family":"He","sequence":"first","affiliation":[{"name":"College of Animal Science and Technology, Northwest A&F University , Yangling, Shaanxi 712100 , People\u2019s Republic of China"},{"name":"Gansu Key Laboratory of Biomonitoring and Bioremediation for Environmental Pollution, School of Life Sciences, Lanzhou University , Lanzhou 730000 , People\u2019s Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiang","family":"Li","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Northwest A&F University , Yangling, Shaanxi 712100 , People\u2019s Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jintao","family":"Li","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Northwest A&F University , Yangling, Shaanxi 712100 , People\u2019s Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yong","family":"Ning","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Northwest A&F University , Yangling, Shaanxi 712100 , People\u2019s Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jun","family":"Luo","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Northwest A&F University , Yangling, Shaanxi 712100 , People\u2019s Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huaiping","family":"Shi","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Northwest A&F University , Yangling, Shaanxi 712100 , People\u2019s Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2024,1,3]]},"reference":[{"key":"2024040612010036600_CIT0001","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1016\/j.anireprosci.2007.06.009","article-title":"Kinetics of fertilization and development, and sex ratio of bovine embryos produced using the semen of different bulls","volume":"107","author":"Alomar","year":"2008","journal-title":"Anim. 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