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This study investigated the hypothesis that HS alters the ovarian proteome and negatively impacts proteins engaged with insulin signaling, inflammation, and ovarian function. Prepubertal gilts (n\u2005=\u200519) were assigned to one of three environmental groups: thermal neutral with ad libitum feed intake (TN; n\u2005=\u20056), thermal neutral pair-fed (PF; n\u2005=\u20056), or HS (n\u2005=\u20057). For 7 d, HS gilts were exposed to 12-h cyclic temperatures of 35.0\u2005\u00b1\u20050.2 \u00b0C and 32.2\u2005\u00b1\u20050.1 \u00b0C, while TN and PF gilts were housed at 21.0\u2005\u00b1\u20050.1 \u00b0C. Liquid chromatography-tandem mass spectrometry (LC-MS\/MS) was performed on ovarian protein homogenates. Relative to TN gilts, 178 proteins were altered (P\u2005\u2264\u20050.05, log2foldchange\u2005\u2265\u20051) by HS, with 76 increased and 102 decreased. STRING gene ontology classified and identified 45 biological processes including those associated with chaperone protein refolding, cytoplasmic translational initiation, and immune activation; with a protein\u2013protein interaction web network of 158 nodes and 563 edges connected based on protein function (FDR\u2005\u2264\u20050.05). Relative to PF, HS altered 330 proteins (P\u2005\u2264\u20050.05, log2foldchange\u2005\u2265\u20051), with 151 increased and 179 decreased. Fifty-seven biological pathways associated with protein function and assembly, RNA processing, and metabolic processes were identified, with a protein\u2013protein interaction network of 303 nodes and 1,606 edges. Comparing HS with both the TN and PF treatments, 72 ovarian proteins were consistently altered by HS with 68 nodes and 104 edges, with biological pathways associated with translation and gene expression. This indicates that HS alters the ovarian proteome and multiple biological pathways and systems in prepubertal gilts; changes that potentially contribute to female infertility.<\/jats:p>","DOI":"10.1093\/jas\/skae053","type":"journal-article","created":{"date-parts":[[2024,3,27]],"date-time":"2024-03-27T14:34:53Z","timestamp":1711550093000},"source":"Crossref","is-referenced-by-count":11,"title":["Heat stress alters the ovarian proteome in prepubertal gilts"],"prefix":"10.1093","volume":"102","author":[{"given":"Crystal M","family":"Roach","sequence":"first","affiliation":[{"name":"Department of Animal Science, Iowa State University , Ames, IA 50011 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edith J","family":"Mayorga","sequence":"additional","affiliation":[{"name":"Department of Animal Science, Iowa State University , Ames, IA 50011 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lance H","family":"Baumgard","sequence":"additional","affiliation":[{"name":"Department of Animal Science, Iowa State University , Ames, IA 50011 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4950-2258","authenticated-orcid":false,"given":"Jason W","family":"Ross","sequence":"additional","affiliation":[{"name":"Department of Animal Science, Iowa State University , Ames, IA 50011 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aileen F","family":"Keating","sequence":"additional","affiliation":[{"name":"Department of Animal Science, Iowa State University , Ames, IA 50011 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2024,4,12]]},"reference":[{"key":"2024041815090707000_CIT0001","doi-asserted-by":"crossref","first-page":"1441","DOI":"10.3168\/jds.2022-22338","article-title":"Effects of dietary antioxidant supplementation on metabolism and inflammatory biomarkers in heat-stressed dairy cows","volume":"106","author":"Abeyta","year":"2023","journal-title":"J. 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