{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,27]],"date-time":"2025-11-27T06:45:53Z","timestamp":1764225953560,"version":"3.41.2"},"reference-count":32,"publisher":"Oxford University Press (OUP)","license":[{"start":{"date-parts":[[2024,3,6]],"date-time":"2024-03-06T00:00:00Z","timestamp":1709683200000},"content-version":"vor","delay-in-days":65,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,1,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Progeny born to primiparous sows (gilt progeny; GP) have lower birth, weaning and slaughter weights than sow progeny (SP). GP also have reduced gastrointestinal tract (GIT) development, as evidenced by lower organ weights. Therefore, the aim of this experiment was to quantify changes in GIT barrier function that occur in birth and weaning, representing two major challenges to the young piglet. The effects of parity (GP vs. SP) in GIT barrier integrity function were quantified at four timepoints: birth (~0\u00a0h), 24\u00a0h after birth (24\u00a0h), 1-d preweaning (PrW), and 1-d postweaning (PoW) in commercially reared piglets. Due to inherent differences between newborn and weanling pigs, the results were analyzed in two cohorts, birth (0 vs. 24\u00a0h, n\u2005=\u200531) and weaning (PrW vs. PoW, n\u2005=\u200540). Samples of the stomach, jejunum, ileum, and colon were excised after euthanasia and barrier integrity was quantified by measuring transepithelial resistance (TER), macromolecular permeability, the abundance of inflammatory proteins (IL-8, IL-1\u03b2, and TNF-\u03b1) and tight junction proteins (claudin-2 and -3). Papp was characterized using a dual tracer approach comprising 4 KDa fluorescein isothiocyanate (FD4) and 150\u00a0kDa tetramethyl rhodamine isothiocyanate (T150)-labeled dextrans. Characteristic effects of the initiation of feeding and weaning were observed on the GIT with the initiation of feeding, such as increasing TER and reducing Papp at 24\u00a0h, consistent with mucosal growth (P\u2005=\u20050.058) This was accompanied by increased cytokine abundance as evidenced by elevations in TNF-\u03b1 and IL-1\u03b2. However, GP had increased IL-8 abundance (P\u2005=\u20050.011 and 0.063 for jejunum and ileum respectively) at birth than 24\u00a0h overall. In the weaning cohort, jejunal and ileal permeability to FD4 was higher in GP (P\u2005=\u20050.05 and 0.022, respectively) while only higher ileal T150 was observed in GP (P\u2005=\u20050.032). Ileal claudin-2 abundance tended to be higher in SP overall (P\u2005=\u20050.063), but GP ileal claudin-2 expression was upregulated weaning while no change was observed in SP (P\u2005=\u20050.043). Finally, other than a higher jejunal TNF-\u03b1 abundance observed in SP (P\u2005=\u20050.016), no other effect of parity was observed on inflammatory markers in the weaning cohort. The results from this study indicate that the GIT of GP have poorer adaptation to early life events, with the response to weaning, being more challenging which is likely to contribute to poorer postweaning growth.<\/jats:p>","DOI":"10.1093\/jas\/skae054","type":"journal-article","created":{"date-parts":[[2024,3,6]],"date-time":"2024-03-06T20:38:32Z","timestamp":1709757512000},"source":"Crossref","is-referenced-by-count":3,"title":["A comparative analysis of gastrointestinal tract barrier function and immune markers in gilt vs. sow progeny at birth and weaning"],"prefix":"10.1093","volume":"102","author":[{"given":"Udani A","family":"Wijesiriwardana","sequence":"first","affiliation":[{"name":"School of Agriculture, Food and Ecosystem Sciences, The University of Melbourne , Parkville, VIC 3010 , Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John R","family":"Pluske","sequence":"additional","affiliation":[{"name":"School of Agriculture, Food and Ecosystem Sciences, The University of Melbourne , Parkville, VIC 3010 , Australia"},{"name":"Australasian Pork Research Institute Ltd , Willaston , SA 5118 , Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0707-6492","authenticated-orcid":false,"given":"Jessica R","family":"Craig","sequence":"additional","affiliation":[{"name":"Research and Innovation, Rivalea (Australia), Pty. Ltd , Corowa, NSW 2646 , Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John B","family":"Furness","sequence":"additional","affiliation":[{"name":"Florey Institute for Neuroscience and Mental Health , Parkville, VIC 3010 , Australia"},{"name":"Department of Anatomy and Neuroscience, The University of Melbourne , Parkville, VIC, 3010 , Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mitchell","family":"Ringuet","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Neuroscience, The University of Melbourne , Parkville, VIC, 3010 , Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Linda J","family":"Fothergill","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Neuroscience, The University of Melbourne , Parkville, VIC, 3010 , Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Frank R","family":"Dunshea","sequence":"additional","affiliation":[{"name":"School of Agriculture, Food and Ecosystem Sciences, The University of Melbourne , Parkville, VIC 3010 , Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1899-2090","authenticated-orcid":false,"given":"Jeremy J","family":"Cottrell","sequence":"additional","affiliation":[{"name":"School of Agriculture, Food and Ecosystem Sciences, The University of Melbourne , Parkville, VIC 3010 , Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2024,3,6]]},"reference":[{"key":"2024032814065797100_CIT0001","doi-asserted-by":"crossref","first-page":"4969","DOI":"10.1242\/jcs.00165","article-title":"Claudin-2 expression induces cation-selective channels in tight junctions of epithelial cells","volume":"115","author":"Amasheh","year":"2002","journal-title":"J. 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