{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,10]],"date-time":"2026-04-10T11:07:18Z","timestamp":1775819238036,"version":"3.50.1"},"reference-count":64,"publisher":"Oxford University Press (OUP)","license":[{"start":{"date-parts":[[2024,1,1]],"date-time":"2024-01-01T00:00:00Z","timestamp":1704067200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,1,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Alternative splicing (AS) plays an important role in the co-transcription and post-transcriptional regulation of gene expression during mammalian spermatogenesis. The dzo is the male F1 offspring of an interspecific hybrid between a domestic bull (Bos taurus \u2642) and a yak (Bos grunniens \u2640) which exhibits male sterility. This study aimed to identify the testis-specific genes and AS associated with hybrid male sterility in dzo. The iDEP90 program and rMATS software were used to identify the differentially expressed genes (DEG) and differential alternative splicing genes (DSG) based on RNA-seq data from the liver (n\u2005=\u20059) and testis (n\u2005=\u20056) tissues of domestic cattle, yak, and dzo. Splicing factors (SF) were obtained from the AmiGO2 and the NCBI databases, and Pearson correlation analysis was performed on the differentially expressed SFs and DSGs. We focused on the testis-specific DEGs and DSGs between dzo and cattle and yak. Among the top 3,000 genes with the most significant variations between these 15 samples, a large number of genes showed testis-specific expression involved with spermatogenesis. Cluster analysis showed that the expression levels of these testis-specific genes were dysregulated during mitosis with a burst downregulation during the pachynema spermatocyte stage. The occurrence of AS events in the testis was about 2.5 fold greater than in the liver, with exon skipping being the major AS event (81.89% to 82.73%). A total of 74 DSGs were specifically expressed in the testis and were significantly enriched during meiosis I, synapsis, and in the piRNA biosynthesis pathways. Notably, STAG3 and DDX4 were of the exon skipping type, and DMC1 was a mutually exclusive exon. A total of 36 SFs were significantly different in dzo testis, compared with cattle and yak. DDX4, SUGP1, and EFTUD2 were potential SFs leading to abnormal AS of testis-specific genes in dzo. These results show that AS of testis-specific genes can affect synapsis and the piRNA biosynthetic processes in dzo, which may be important factors associated with hybrid male sterility in dzo.<\/jats:p>","DOI":"10.1093\/jas\/skae091","type":"journal-article","created":{"date-parts":[[2024,3,29]],"date-time":"2024-03-29T08:08:12Z","timestamp":1711699692000},"source":"Crossref","is-referenced-by-count":3,"title":["mRNA-Seq of testis and liver tissues reveals a testis-specific gene and alternative splicing associated with hybrid male sterility in dzo"],"prefix":"10.1093","volume":"102","author":[{"given":"Rui","family":"Hong","sequence":"first","affiliation":[{"name":"College of Animal Science and Technology, Southwest University , Rongchang, 402460 Chongqing , China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiaxin","family":"Wu","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Southwest University , Rongchang, 402460 Chongqing , China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xining","family":"Chen","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Southwest University , Rongchang, 402460 Chongqing , China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhenghao","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Southwest University , Rongchang, 402460 Chongqing , China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xinyue","family":"Liu","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Southwest University , Rongchang, 402460 Chongqing , China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Meichen","family":"Li","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Southwest University , Rongchang, 402460 Chongqing , China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fuyuan","family":"Zuo","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Southwest University , Rongchang, 402460 Chongqing , China"},{"name":"Beef Cattle Engineering and Technology Research Center of Chongqing, Southwest University , Rongchang, 402460 Chongqing , China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gong-Wei","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Southwest University , Rongchang, 402460 Chongqing , China"},{"name":"Beef Cattle Engineering and Technology Research Center of Chongqing, Southwest University , Rongchang, 402460 Chongqing , China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2024,3,29]]},"reference":[{"key":"2024070118223717100_CIT0001","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1016\/j.mcp.2016.03.001","article-title":"Exon skipping creates novel splice variants of DMC1 gene in ruminants","volume":"30","author":"Ahlawat","year":"2016","journal-title":"Mol. Cell. Probes"},{"key":"2024070118223717100_CIT0002","doi-asserted-by":"crossref","first-page":"1587","DOI":"10.1126\/science.1230612","article-title":"The evolutionary landscape of alternative splicing in vertebrate species","volume":"338","author":"Barbosa-Morais","year":"2012","journal-title":"Science"},{"key":"2024070118223717100_CIT0003","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1016\/j.theriogenology.2016.09.047","article-title":"Comparative analysis of testis transcriptomes associated with male infertility in cattleyak","volume":"88","author":"Cai","year":"2017","journal-title":"Theriogenology"},{"key":"2024070118223717100_CIT0004","doi-asserted-by":"crossref","DOI":"10.3390\/ijms232415810","article-title":"Analysis of chromatin openness in testicle tissue of Yak and Cattle-Yak","volume":"23","author":"Cao","year":"2022","journal-title":"Int. J. Mol. Sci."},{"key":"2024070118223717100_CIT0005","doi-asserted-by":"crossref","first-page":"240","DOI":"10.1038\/s41419-020-2443-y","article-title":"Combinatorial control of Spo11 alternative splicing by modulation of RNA polymerase II dynamics and splicing factor recruitment during meiosis","volume":"11","author":"Cesari","year":"2020","journal-title":"Cell Death Dis"},{"key":"2024070118223717100_CIT0007","doi-asserted-by":"crossref","first-page":"741","DOI":"10.1038\/nrm2777","article-title":"Mechanisms of alternative splicing regulation: insights from molecular and genomics approaches","volume":"10","author":"Chen","year":"2009","journal-title":"Nat. Rev. Mol. Cell Biol"},{"key":"2024070118223717100_CIT0006","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1016\/j.theriogenology.2022.04.022","article-title":"N6-methyladenosine RNA demethylase ALKBH5 is testis-specifically downregulated in hybrid male sterile dzo and is a target gene of bta-miR-200a","volume":"187","author":"Chen","year":"2022","journal-title":"Theriogenology"},{"key":"2024070118223717100_CIT0008","doi-asserted-by":"crossref","first-page":"176","DOI":"10.1007\/s00239-016-9734-z","article-title":"Misregulation of gene expression and sterility in interspecies hybrids: Causal links and alternative hypotheses","volume":"82","author":"Civetta","year":"2016","journal-title":"J. Mol. Evol"},{"key":"2024070118223717100_CIT0009","doi-asserted-by":"crossref","first-page":"294","DOI":"10.1186\/s12864-016-2618-1","article-title":"Transcriptome analysis of highly purified mouse spermatogenic cell populations: gene expression signatures switch from meiotic-to postmeiotic-related processes at pachytene stage","volume":"17","author":"da Cruz","year":"2016","journal-title":"BMC Genomics"},{"key":"2024070118223717100_CIT0010","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1016\/j.semcdb.2014.04.021","article-title":"Regulation of spermatogenesis by small non-coding RNAs: Role of the germ granule","volume":"29","author":"de Mateo","year":"2014","journal-title":"Semin Cell Dev Biol"},{"key":"2024070118223717100_CIT0011","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1038\/s41421-023-00577-5","article-title":"A novel recombination protein C12ORF40\/REDIC1 is required for meiotic crossover formation","volume":"9","author":"Fan","year":"2023","journal-title":"Cell Discov"},{"key":"2024070118223717100_CIT0012","doi-asserted-by":"crossref","first-page":"3588","DOI":"10.1038\/s41467-022-31364-7","article-title":"hnRNPH1 recruits PTBP2 and SRSF3 to modulate alternative splicing in germ cells","volume":"13","author":"Feng","year":"2022","journal-title":"Nat. Commun"},{"key":"2024070118223717100_CIT0013","doi-asserted-by":"crossref","first-page":"689","DOI":"10.1038\/nrg3778","article-title":"Context-dependent control of alternative splicing by RNA-binding proteins","volume":"15","author":"Fu","year":"2014","journal-title":"Nat. Rev. Genet"},{"key":"2024070118223717100_CIT0014","doi-asserted-by":"crossref","first-page":"534","DOI":"10.1186\/s12859-018-2486-6","article-title":"iDEP: an integrated web application for differential expression and pathway analysis of RNA-Seq data","volume":"19","author":"Ge","year":"2018","journal-title":"BMC Bioinf"},{"key":"2024070118223717100_CIT0015","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1152\/physrev.00013.2015","article-title":"Spermatogenesis: The commitment to meiosis","volume":"96","author":"Griswold","year":"2016","journal-title":"Physiol. Rev"},{"key":"2024070118223717100_CIT0016","doi-asserted-by":"crossref","first-page":"664260","DOI":"10.3389\/fgene.2021.664260","article-title":"Large-scale multiplexing permits full-length transcriptome annotation of 32 bovine tissues from a single nanopore flow cell","volume":"12","author":"Halstead","year":"2021","journal-title":"Front. Genet"},{"key":"2024070118223717100_CIT0017","doi-asserted-by":"crossref","first-page":"2598","DOI":"10.1016\/j.celrep.2017.05.089","article-title":"Ptbp2 controls an alternative splicing network required for cell communication during spermatogenesis","volume":"19","author":"Hannigan","year":"2017","journal-title":"Cell Rep"},{"key":"2024070118223717100_CIT0018","doi-asserted-by":"crossref","first-page":"677","DOI":"10.1126\/science.abj9090","article-title":"Exon architecture controls mRNA m6A suppression and gene expression","volume":"379","author":"He","year":"2023","journal-title":"Science"},{"key":"2024070118223717100_CIT0019","doi-asserted-by":"crossref","first-page":"689","DOI":"10.1016\/j.molcel.2020.06.015","article-title":"The configuration of RPA, RAD51, and DMC1 binding in meiosis reveals the nature of critical recombination intermediates","volume":"79","author":"Hinch","year":"2020","journal-title":"Mol. Cell"},{"key":"2024070118223717100_CIT0020","doi-asserted-by":"crossref","first-page":"1023","DOI":"10.1083\/jcb.201304004","article-title":"A mammalian KASH domain protein coupling meiotic chromosomes to the cytoskeleton","volume":"202","author":"Horn","year":"2013","journal-title":"J. Cell Biol"},{"key":"2024070118223717100_CIT0021","doi-asserted-by":"crossref","first-page":"291","DOI":"10.4141\/cjas2012-018","article-title":"Quantitation of alternative splicing variants of lactate dehydrogenase C gene in testes of adult yak, sexually immature yak calf and sterile male hybrid of yak","volume":"92","author":"Huang","year":"2012","journal-title":"Can. J. Anim. Sci"},{"key":"2024070118223717100_CIT0022","doi-asserted-by":"crossref","first-page":"715","DOI":"10.1038\/nrg3052","article-title":"Functional consequences of developmentally regulated alternative splicing","volume":"12","author":"Kalsotra","year":"2011","journal-title":"Nat. Rev. Genet"},{"key":"2024070118223717100_CIT0023","doi-asserted-by":"crossref","first-page":"916","DOI":"10.1126\/science.1195774","article-title":"Distinct properties of the XY pseudoautosomal region crucial for male meiosis","volume":"331","author":"Kauppi","year":"2011","journal-title":"Science"},{"key":"2024070118223717100_CIT0024","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1038\/nmeth.3317","article-title":"HISAT: a fast spliced aligner with low memory requirements","volume":"12","author":"Kim","year":"2015","journal-title":"Nat. Methods"},{"key":"2024070118223717100_CIT0025","doi-asserted-by":"crossref","first-page":"887","DOI":"10.1101\/gad.1902110","article-title":"MVH in piRNA processing and gene silencing of retrotransposons","volume":"24","author":"Kuramochi-Miyagawa","year":"2010","journal-title":"Genes Dev"},{"key":"2024070118223717100_CIT0026","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1186\/s12915-023-01736-6","article-title":"SRSF2 is required for mRNA splicing during spermatogenesis","volume":"21","author":"Lei","year":"2023","journal-title":"BMC Biol"},{"key":"2024070118223717100_CIT0027","doi-asserted-by":"crossref","first-page":"4883","DOI":"10.7150\/ijbs.83474","article-title":"SRSF1-mediated alternative splicing is required for spermatogenesis","volume":"19","author":"Lei","year":"2023","journal-title":"Int. J. Biol. Sci"},{"key":"2024070118223717100_CIT0029","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1016\/j.molcel.2013.02.016","article-title":"An ancient transcription factor initiates the burst of piRNA production during early meiosis in mouse testes","volume":"50","author":"Li","year":"2013","journal-title":"Mol. Cell"},{"key":"2024070118223717100_CIT0028","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1016\/j.gene.2014.08.028","article-title":"Identification and characterization of yak (Bos grunniens) b-Boule gene and its alternative splice variants","volume":"550","author":"Li","year":"2014","journal-title":"Gene"},{"key":"2024070118223717100_CIT0030","doi-asserted-by":"crossref","first-page":"164","DOI":"10.1016\/j.theriogenology.2020.01.001","article-title":"The expression of histone methyltransferases and distribution of selected histone methylations in testes of yak and cattle-yak hybrid","volume":"144","author":"Li","year":"2020","journal-title":"Theriogenology"},{"key":"2024070118223717100_CIT0031","doi-asserted-by":"crossref","first-page":"14182","DOI":"10.1038\/ncomms14182","article-title":"BCAS2 is involved in alternative mRNA splicing in spermatogonia and the","volume":"8","author":"Liu","year":"2017","journal-title":"Nat. Commun"},{"key":"2024070118223717100_CIT0032","doi-asserted-by":"crossref","first-page":"3421","DOI":"10.1093\/hmg\/ddu051","article-title":"STAG3 is a strong candidate gene for male infertility","volume":"23","author":"Llano","year":"2014","journal-title":"Hum. Mol. Genet"},{"key":"2024070118223717100_CIT0033","doi-asserted-by":"crossref","first-page":"208","DOI":"10.1016\/j.livsci.2013.12.017","article-title":"Histological evaluation and Prdm9 expression level in the testis of sterile male cattle-yaks","volume":"160","author":"Lou","year":"2014","journal-title":"Livest. Sci"},{"key":"2024070118223717100_CIT0034","doi-asserted-by":"crossref","first-page":"550","DOI":"10.1186\/s13059-014-0550-8","article-title":"Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2","volume":"15","author":"Love","year":"2014","journal-title":"Genome Biol"},{"key":"2024070118223717100_CIT0035","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1186\/1471-2156-14-58","article-title":"Splice variants and promoter methylation status of the Bovine Vasa Homology (Bvh) gene may be involved in bull spermatogenesis","volume":"14","author":"Luo","year":"2013","journal-title":"BMC Genet"},{"key":"2024070118223717100_CIT0036","doi-asserted-by":"crossref","first-page":"eabk1789","DOI":"10.1126\/sciadv.abk1789","article-title":"RAD51AP2 is required for efficient meiotic recombination between X and Y chromosomes","volume":"8","author":"Ma","year":"2022","journal-title":"Sci. Adv"},{"key":"2024070118223717100_CIT0037","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1016\/j.tig.2016.11.003","article-title":"Gene regulation and speciation","volume":"33","author":"Mack","year":"2017","journal-title":"Trends. Genet"},{"key":"2024070118223717100_CIT0038","doi-asserted-by":"crossref","first-page":"1593","DOI":"10.1126\/science.1228186","article-title":"Evolutionary dynamics of gene and isoform regulation in mammalian tissues","volume":"338","author":"Merkin","year":"2012","journal-title":"Science"},{"key":"2024070118223717100_CIT0039","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1186\/s12864-023-09251-2","article-title":"Single-cell transcriptome analysis and in vitro differentiation of testicular cells reveal novel insights into male sterility of the interspecific hybrid cattle-yak","volume":"24","author":"Mipam","year":"2023","journal-title":"BMC Genomics"},{"key":"2024070118223717100_CIT0040","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1186\/s13072-018-0242-9","article-title":"EZH2 variants differentially regulate polycomb repressive complex 2 in histone methylation and cell differentiation","volume":"11","author":"Mu","year":"2018","journal-title":"Epigenet. Chromatin"},{"key":"2024070118223717100_CIT0041","doi-asserted-by":"crossref","first-page":"495","DOI":"10.1093\/biolre\/ioaa207","article-title":"Male hybrid sterility in the cattle-yak and other bovines: a review","volume":"104","author":"Niayale","year":"2021","journal-title":"Biol. Reprod"},{"key":"2024070118223717100_CIT0042","doi-asserted-by":"crossref","first-page":"304","DOI":"10.1111\/rda.14061","article-title":"Aberrant levels of DNA methylation and H3K9 acetylation in the testicular cells of crossbred cattle-yak showing infertility","volume":"57","author":"Phakdeedindan","year":"2022","journal-title":"Reprod. Domest. Anim"},{"key":"2024070118223717100_CIT0043","doi-asserted-by":"crossref","first-page":"10170","DOI":"10.1093\/nar\/gkt811","article-title":"The splicing landscape is globally reprogrammed during male meiosis","volume":"41","author":"Schmid","year":"2013","journal-title":"Nucleic Acids Res"},{"key":"2024070118223717100_CIT0044","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1262\/jrd.2018-109","article-title":"PTBP1 contributes to spermatogenesis through regulation of proliferation in spermatogonia","volume":"65","author":"Senoo","year":"2019","journal-title":"J. Reprod. Dev"},{"key":"2024070118223717100_CIT0045","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1093\/biolre\/ioz056","article-title":"GPAT2 is required for piRNA biogenesis, transposon silencing, and maintenance of spermatogonia in mice","volume":"101","author":"Shiromoto","year":"2019","journal-title":"Biol. Reprod"},{"key":"2024070118223717100_CIT0046","doi-asserted-by":"crossref","first-page":"38","DOI":"10.7150\/ijbs.34422","article-title":"The function of pre-mRNA alternative splicing in mammal spermatogenesis","volume":"16","author":"Song","year":"2020","journal-title":"Int. J. Biol. Sci"},{"key":"2024070118223717100_CIT0047","doi-asserted-by":"crossref","first-page":"2179","DOI":"10.1016\/j.celrep.2013.05.031","article-title":"Cellular source and mechanisms of high transcriptome complexity in the mammalian testis","volume":"3","author":"Soumillon","year":"2013","journal-title":"Cell Rep"},{"key":"2024070118223717100_CIT0048","doi-asserted-by":"crossref","first-page":"E325","DOI":"10.1073\/pnas.1717794115","article-title":"ALKBH5-dependent m6A demethylation controls splicing and stability of long 3\u02b9-UTR mRNAs in male germ cells","volume":"115","author":"Tang","year":"2018","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2024070118223717100_CIT0049","first-page":"2112","article-title":"Mutations in the stromal antigen 3 (STAG3) gene cause male infertility due to meiotic arrest","volume":"34","author":"van der Bijl","year":"2019","journal-title":"Hum. Reprod"},{"key":"2024070118223717100_CIT0050","doi-asserted-by":"crossref","first-page":"971515","DOI":"10.3389\/fvets.2022.971515","article-title":"Characterization of N(6)-methyladenosine in cattle-yak testis tissue","volume":"9","author":"Wang","year":"2022","journal-title":"Front Vet Sci"},{"key":"2024070118223717100_CIT0051","doi-asserted-by":"crossref","first-page":"623","DOI":"10.1016\/j.devcel.2017.05.021","article-title":"Distinct Roles of RNA Helicases MVH and TDRD9 in PIWI slicing-triggered mammalian piRNA biogenesis and function","volume":"41","author":"Wenda","year":"2017","journal-title":"Dev. Cell"},{"key":"2024070118223717100_CIT0052","doi-asserted-by":"crossref","first-page":"a002626","DOI":"10.1101\/cshperspect.a002626","article-title":"Unique aspects of transcription regulation in male germ cells","volume":"3","author":"White-Cooper","year":"2011","journal-title":"Cold Spring Harbor Perspect. Biol"},{"key":"2024070118223717100_CIT0055","doi-asserted-by":"crossref","first-page":"e0229503","DOI":"10.1371\/journal.pone.0229503","article-title":"Testis transcriptome profiling identified genes involved in spermatogenic arrest of cattleyak","volume":"15","author":"Wu","year":"2020","journal-title":"PLoS One"},{"key":"2024070118223717100_CIT0053","doi-asserted-by":"crossref","first-page":"162","DOI":"10.1080\/15476286.2021.2024033","article-title":"Alternative splicing and MicroRNA: epigenetic mystique in male reproduction","volume":"19","author":"Wu","year":"2022","journal-title":"RNA Biol"},{"key":"2024070118223717100_CIT0054","doi-asserted-by":"crossref","first-page":"e2300107","DOI":"10.1002\/pmic.202300107","article-title":"Comparative proteomic analysis identifies differentially expressed proteins associated with meiotic","volume":"23","author":"Wu","year":"2023","journal-title":"Proteomics"},{"key":"2024070118223717100_CIT0056","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1016\/j.theriogenology.2023.03.017","article-title":"Transcriptome analysis reveals dysregulated gene expression networks in Sertoli cells of cattle-yak hybrids","volume":"203","author":"Wu","year":"2023","journal-title":"Theriogenology"},{"key":"2024070118223717100_CIT0057","doi-asserted-by":"crossref","first-page":"e22918","DOI":"10.1096\/fj.202202152RR","article-title":"SRSF2 in Sertoli cells is essential for testicular development and spermatogenesis in mice","volume":"37","author":"Xie","year":"2023","journal-title":"FASEB J"},{"key":"2024070118223717100_CIT0058","doi-asserted-by":"crossref","first-page":"239","DOI":"10.7150\/ijbs.38232","article-title":"Bovid microRNAs involved in the process of spermatogonia differentiation into spermatocytes","volume":"16","author":"Xu","year":"2020","journal-title":"Int. J. Biol. Sci"},{"key":"2024070118223717100_CIT0059","doi-asserted-by":"crossref","first-page":"gaac021","DOI":"10.1093\/molehr\/gaac021","article-title":"Novel bi-allelic variants in KASH5 are associated with meiotic arrest and non-obstructive azoospermia","volume":"28","author":"Yang","year":"2022","journal-title":"Mol. Hum. Reprod"},{"key":"2024070118223717100_CIT0060","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1261\/rna.079472.122","article-title":"A-MYB\/TCFL5 regulatory architecture ensures the production of pachytene piRNAs in placental mammals","volume":"29","author":"Yu","year":"2023","journal-title":"RNA"},{"key":"2024070118223717100_CIT0063","doi-asserted-by":"crossref","first-page":"6263","DOI":"10.3168\/jds.2018-15680","article-title":"Comparison of Y-chromosome-linked TSPY, TSPY2, and PRAMEY genes in Taurus cattle, yaks, and interspecific hybrid bulls","volume":"102","author":"Zhang","year":"2019","journal-title":"J. Dairy Sci"},{"key":"2024070118223717100_CIT0064","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1016\/j.molcel.2019.07.017","article-title":"Disease-causing mutations in SF3B1 alter splicing by disrupting interaction with SUGP1","volume":"76","author":"Zhang","year":"2019","journal-title":"Mol. Cell"},{"key":"2024070118223717100_CIT0061","doi-asserted-by":"crossref","first-page":"914","DOI":"10.1080\/15592294.2020.1738026","article-title":"Promoter hypermethylation of PIWI\/piRNA pathway genes associated with diminished pachytene piRNA production in bovine hybrid male sterility","volume":"15","author":"Zhang","year":"2020","journal-title":"Epigenetics"},{"key":"2024070118223717100_CIT0062","doi-asserted-by":"crossref","first-page":"4366","DOI":"10.3168\/jds.2022-22963","article-title":"Evaluation of MYBL1 as the master regulator for pachytene spermatocyte genes dysregulated in interspecific hybrid dzo","volume":"106","author":"Zhang","year":"2023","journal-title":"J. Dairy Sci"}],"container-title":["Journal of Animal Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/jas\/advance-article-pdf\/doi\/10.1093\/jas\/skae091\/57117567\/skae091.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jas\/article-pdf\/doi\/10.1093\/jas\/skae091\/58381790\/skae091.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jas\/article-pdf\/doi\/10.1093\/jas\/skae091\/58381790\/skae091.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,7,1]],"date-time":"2024-07-01T18:32:06Z","timestamp":1719858726000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jas\/article\/doi\/10.1093\/jas\/skae091\/7637297"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,1,1]]},"references-count":64,"URL":"https:\/\/doi.org\/10.1093\/jas\/skae091","relation":{},"ISSN":["0021-8812","1525-3163"],"issn-type":[{"value":"0021-8812","type":"print"},{"value":"1525-3163","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2024,1,1]]},"published":{"date-parts":[[2024,1,1]]},"article-number":"skae091"}}