{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T11:55:12Z","timestamp":1753876512523,"version":"3.41.2"},"reference-count":44,"publisher":"Oxford University Press (OUP)","license":[{"start":{"date-parts":[[2024,1,1]],"date-time":"2024-01-01T00:00:00Z","timestamp":1704067200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,1,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Infertility or subfertility impacts approximately 5% and 15% of dairy and beef heifers (Bos taurus), respectively. Heifers that do not produce a calf within an optimum window of time have a significant negative impact on the profitability and sustainability of the cattle industry. Selection of heifers based on their fertility potential remains a challenge yet to be resolved. Here, we tested the hypothesis that heifers of different fertility potential have differing metabolome signatures in their plasma. We obtained blood from Bos taurus heifers at their first artificial insemination and processed the samples to separate the plasma. The heifers were classified based on their reproductive outcome as fertile (pregnant and delivered a calf after their first artificial insemination (AI)) or sub-fertile (Angus heifers: no pregnancy after two AI and exposure to a bull; Holstein heifers: no pregnancy by the third AI). We tested the relative abundance of 140 metabolites obtained from 22 heifers (Angus fertile n\u2005=\u20055, Angus sub-fertile n\u2005=\u20057, Holstein fertile N\u2005=\u20055, Holstein sub-fertile N\u2005=\u20055). The metabolite 2-Dehydro-D-gluconate (C6H10O7) was significantly more abundant in the plasma of sub-fertile heifers in both breeds (1.4-fold, false discovery rate\u2005&amp;lt;\u20050.1). In the context that a small proportion of circulating metabolites in the plasma were quantified in this study, the results show that the metabolomic profile in the blood stream may be associated with heifer fertility potential.<\/jats:p>","DOI":"10.1093\/jas\/skae126","type":"journal-article","created":{"date-parts":[[2024,5,9]],"date-time":"2024-05-09T07:20:43Z","timestamp":1715239243000},"source":"Crossref","is-referenced-by-count":0,"title":["Higher abundance of 2-dehydro-<scp>d<\/scp>-gluconate in the plasma of sub-fertile or infertile <i>Bos taurus<\/i> heifers"],"prefix":"10.1093","volume":"102","author":[{"given":"Mackenzie","family":"Marrella","sequence":"first","affiliation":[{"name":"School of Animal Sciences, Virginia Polytechnique Institute and State University , Blacksburg, VA 24061 , USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9243-3863","authenticated-orcid":false,"given":"Sarah E D","family":"Moorey","sequence":"additional","affiliation":[{"name":"Department of Animal Science, University of Tennessee , Knoxville, TN 37996 , USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shawn R","family":"Campagna","sequence":"additional","affiliation":[{"name":"Department of Chemistry, University of Tennessee , Knoxville, TN 37919 , USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qudus","family":"Sarumi","sequence":"additional","affiliation":[{"name":"Department of Chemistry, University of Tennessee , Knoxville, TN 37919 , USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7895-0223","authenticated-orcid":false,"given":"Fernando H","family":"Biase","sequence":"additional","affiliation":[{"name":"School of Animal Sciences, Virginia Polytechnique Institute and State University , Blacksburg, VA 24061 , USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2024,5,9]]},"reference":[{"key":"2024071512311135900_CIT0001","doi-asserted-by":"crossref","first-page":"e201301009","DOI":"10.5936\/csbj.201301009","article-title":"Statistical methods for the analysis of high-throughput metabolomics data","volume":"4","author":"Bartel","year":"2013","journal-title":"Comput. 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B Methodol"},{"key":"2024071512311135900_CIT0004","doi-asserted-by":"crossref","first-page":"185","DOI":"10.4236\/ojas.2015.52021","article-title":"A study of dairy heifer rearing practices from birth to weaning and their associated costs on UK dairy farms","volume":"05","author":"Boulton","year":"2015","journal-title":"Open J. Anim. Sci"},{"key":"2024071512311135900_CIT0005","doi-asserted-by":"crossref","first-page":"4486","DOI":"10.2527\/jas.2013-6465","article-title":"Heifer calving date positively influences calf weaning weights through six parturitions","volume":"91","author":"Cushman","year":"2013","journal-title":"J. Anim. Sci"},{"key":"2024071512311135900_CIT0006","doi-asserted-by":"crossref","first-page":"S61","DOI":"10.1093\/tas\/txy020","article-title":"Effect of calving period on beef cow longevity and lifetime productivity in western Canada","volume":"2","author":"Damiran","year":"2018","journal-title":"Transl. Anim. Sci"},{"key":"2024071512311135900_CIT0007","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1016\/j.theriogenology.2020.01.041","article-title":"Breeding animals to feed people: the many roles of animal reproduction in ensuring global food security","volume":"150","author":"Davis","year":"2020","journal-title":"Theriogenology"},{"key":"2024071512311135900_CIT0008","doi-asserted-by":"crossref","first-page":"706","DOI":"10.1016\/j.dib.2018.03.062","article-title":"Transcriptome data of peripheral white blood cells from beef heifers collected at the time of artificial insemination","volume":"18","author":"Dickinson","year":"2018","journal-title":"Data Brief"},{"key":"2024071512311135900_CIT0009","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1186\/s12864-018-4505-4","article-title":"Transcriptome profiles in peripheral white blood cells at the time of artificial insemination discriminate beef heifers with different fertility potential","volume":"19","author":"Dickinson","year":"2018","journal-title":"BMC Genomics"},{"key":"2024071512311135900_CIT0010","doi-asserted-by":"crossref","first-page":"1560","DOI":"10.1038\/s42255-022-00670-1","article-title":"Genome-microbiome interplay provides insight into the determinants of the human blood metabolome","volume":"4","author":"Diener","year":"2022","journal-title":"Nat. Metab"},{"key":"2024071512311135900_CIT0011","doi-asserted-by":"crossref","first-page":"767","DOI":"10.3390\/genes11070767","article-title":"Identification of loci and pathways associated with heifer conception rate in U.S. Holsteins","volume":"11","author":"Galliou","year":"2020","journal-title":"Genes (Basel)"},{"key":"2024071512311135900_CIT0012","doi-asserted-by":"crossref","first-page":"3179","DOI":"10.3168\/jds.S0022-0302(93)77656-0","article-title":"Raising dairy replacements to meet the needs of the 21st century","volume":"76","author":"Heinrichs","year":"1993","journal-title":"J. Dairy Sci"},{"key":"2024071512311135900_CIT0013","doi-asserted-by":"crossref","first-page":"836","DOI":"10.2527\/1997.753836x","article-title":"Optimum body size of Holstein replacement heifers","volume":"75","author":"Hoffman","year":"1997","journal-title":"J. Anim. 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Dairy Sci"},{"key":"2024071512311135900_CIT0019","doi-asserted-by":"crossref","first-page":"1","DOI":"10.2527\/jas1973.3611","article-title":"Date of first calving in beef cows and subsequent calf production","volume":"36","author":"Lesmeister","year":"1973","journal-title":"J. Anim. Sci"},{"key":"2024071512311135900_CIT0020","doi-asserted-by":"crossref","first-page":"152","DOI":"10.1080\/01652176.2014.962116","article-title":"Plasma metabolic profiling of dairy cows affected with clinical ketosis using LC\/MS technology","volume":"34","author":"Li","year":"2014","journal-title":"Vet. Q"},{"key":"2024071512311135900_CIT0021","doi-asserted-by":"crossref","first-page":"7054","DOI":"10.3168\/jds.2013-7093","article-title":"Hormonal manipulations in the 5-day timed artificial insemination protocol to optimize estrous cycle synchrony and fertility in dairy heifers","volume":"96","author":"Lima","year":"2013","journal-title":"J. Dairy Sci"},{"key":"2024071512311135900_CIT0022","doi-asserted-by":"crossref","first-page":"274","DOI":"10.1016\/j.tibs.2017.01.004","article-title":"Metabolomics: a primer","volume":"42","author":"Liu","year":"2017","journal-title":"Trends Biochem. Sci"},{"key":"2024071512311135900_CIT0023","doi-asserted-by":"crossref","first-page":"3212","DOI":"10.1021\/ac902837x","article-title":"Metabolomic analysis via reversed-phase ion-pairing liquid chromatography coupled to a stand alone orbitrap mass spectrometer","volume":"82","author":"Lu","year":"2010","journal-title":"Anal. Chem"},{"key":"2024071512311135900_CIT0024","doi-asserted-by":"crossref","first-page":"12664","DOI":"10.1038\/s41598-023-39858-0","article-title":"A multi-omics analysis identifies molecular features associated with fertility in heifers (Bos taurus)","volume":"13","author":"Marrella","year":"2023","journal-title":"Sci. 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