{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T01:20:43Z","timestamp":1791249643301,"version":"4.1.0"},"reference-count":45,"publisher":"Oxford University Press (OUP)","license":[{"start":{"date-parts":[[2024,1,1]],"date-time":"2024-01-01T00:00:00Z","timestamp":1704067200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,1,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Adding multienzymes to poultry feed rations is recognized as a nutritional strategy aimed at improving poultry performance and health status. Nonetheless, some literatures present an ongoing debate about the extent of multienzymes beneficial impact on poultry growth performance. This study aimed to explore the impacts of dietary multienzyme supplementation on broilers, focusing specifically on growth performance, carcass characteristics, apparent nutrient digestibility, excreta noxious gas emission, and intestinal nutrient transporter gene expression. A total of 3,200 broilers were randomly assigned to five groups (eight replicates per treatment group) and treated with the following: normal control (CON), CON\u2005+\u2005100\u00a0g\/t multienzyme (ME100), CON\u2005+\u2005150\u00a0g\/t multienzyme (ME150), CON\u2005+\u2005200\u00a0g\/t multienzyme (ME200), and CON\u2005+\u2005250\u00a0g\/t multienzyme (ME250). Supplementing with multienzymes significantly influenced the feed conversion rate (linear, P\u2005=\u20050.007; quadratic, P\u2005=\u20050.024) and the European broiler index (linear, P\u2005=\u20050.004; quadratic, P\u2005=\u20050.016) in broilers. Dietary multienzymes significantly influenced apparent metabolizable energy (quadratic, P\u2005=\u20050.015) and neutral detergent fiber (quadratic, P\u2005&amp;lt;\u20050.001). Moreover, multienzyme supplementation in the diet also decreased the emission of ammonia (linear, P\u2005=\u20050.001; quadratic, P\u2005=\u20050.006) and hydrogen sulfide (quadratic, P\u2005=\u20050.006) in the excreta. In addition, dietary multi-enzyme notably elevated (P\u2005&amp;lt;\u20050.05) the mRNA expression of nutrient transporter genes, including peptide transporter 1 (PePT1), Na-dependent neutral amino acid transporter (B0AT), glucose transporter 2 (GLUT2), and fatty acid binding protein1 (FABP1). These findings suggest that dietary supplementation with multienzymes can improve the efficiency of feed utilization, and the digestion and absorption of nutrients and reduce excreta gas emission. Furthermore, this study provides a theoretical basis for advancing the use of multienzymes in broiler production.<\/jats:p>","DOI":"10.1093\/jas\/skae133","type":"journal-article","created":{"date-parts":[[2024,5,11]],"date-time":"2024-05-11T07:40:45Z","timestamp":1715413245000},"source":"Crossref","is-referenced-by-count":17,"title":["Effects of dietary multienzymes on the growth performance, digestive enzyme activity, nutrient digestibility, excreta noxious gas emission, and nutrient transporter gene expression in white feather broilers"],"prefix":"10.1093","volume":"102","author":[{"given":"Wuzhou","family":"Yi","sequence":"first","affiliation":[{"name":"College of Animal Sciences, Zhejiang University , Hangzhou , China"},{"name":"The Key Laboratory of Molecular Animal Nutrition, Ministry of Education , Hangzhou , China"},{"name":"Zhejiang Provincial Laboratory of Feed and Animal Nutrition , Hangzhou , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qixin","family":"Huang","sequence":"additional","affiliation":[{"name":"College of Animal Sciences, Zhejiang University , Hangzhou , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanjie","family":"Liu","sequence":"additional","affiliation":[{"name":"Jinan Bestzyme Bio-Engineering Co., Ltd , Jinan , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shijun","family":"Fu","sequence":"additional","affiliation":[{"name":"Shandong Binzhou Animal Science & Veterinary Medicine Academy , Binzhou , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4738-414X","authenticated-orcid":false,"given":"Tizhong","family":"Shan","sequence":"additional","affiliation":[{"name":"College of Animal Sciences, Zhejiang University , Hangzhou , China"},{"name":"The Key Laboratory of Molecular Animal Nutrition, Ministry of Education , Hangzhou , China"},{"name":"Zhejiang Provincial Laboratory of Feed and Animal Nutrition , Hangzhou , China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2024,5,11]]},"reference":[{"key":"2024060513273077100_CIT0001","doi-asserted-by":"crossref","first-page":"150","DOI":"10.1080\/1745039X.2017.1283823","article-title":"Feeding value of field beans (Vicia faba L. var. minor) with and without enzyme containing tannase, pectinase and xylanase activities for broilers","volume":"71","author":"Abdulla","year":"2017","journal-title":"Arch. Anim. Nutr"},{"key":"2024060513273077100_CIT0002","doi-asserted-by":"crossref","first-page":"3189","DOI":"10.2527\/jas.2010-3715","article-title":"BOARD-INVITED REVIEW: opportunities and challenges in using exogenous enzymes to improve nonruminant animal production","volume":"89","author":"Adeola","year":"2011","journal-title":"J. Anim. Sci"},{"key":"2024060513273077100_CIT0003","doi-asserted-by":"crossref","first-page":"G897","DOI":"10.1152\/ajpgi.00454.2004","article-title":"Immediate early genes of glucocorticoid action on the developing intestine","volume":"288","author":"Agbemafle","year":"2005","journal-title":"Am. J. Physiol. Gastrointest. Liver Physiol"},{"key":"2024060513273077100_CIT0004","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1186\/s12917-021-02946-2","article-title":"Effects of different feeding regimens with protease supplementation on growth, amino acid digestibility, economic efficiency, blood biochemical parameters, and intestinal histology in broiler chickens","volume":"17","author":"Amer","year":"2021","journal-title":"BMC Vet. Res"},{"key":"2024060513273077100_CIT0005","doi-asserted-by":"crossref","first-page":"807","DOI":"10.3382\/ps\/pew297","article-title":"Effect of exogenous xylanase, amylase, and protease as single or combined activities on nutrient digestibility and growth performance of broilers fed corn\/soy diets","volume":"96","author":"Amerah","year":"2017","journal-title":"Poult. Sci"},{"key":"2024060513273077100_CIT0006","doi-asserted-by":"crossref","first-page":"1012462","DOI":"10.3389\/fvets.2022.1012462","article-title":"The impact of multi-enzyme fortification on growth performance, intestinal morphology, nutrient digestibility, and meat quality of broiler chickens fed a standard or low-density diet","volume":"9","author":"Attia","year":"2022","journal-title":"Front. Vet. Sci"},{"key":"2024060513273077100_CIT0007","doi-asserted-by":"crossref","first-page":"1050","DOI":"10.1111\/jpn.12476","article-title":"Effect of dietary lignocellulose on ileal and total tract digestibility of fat and fatty acids in broiler chickens","volume":"100","author":"Bogus\u0142awska-Tryk","year":"2016","journal-title":"J. Anim. Physiol. Anim. Nutr. (Berl)"},{"key":"2024060513273077100_CIT0008","doi-asserted-by":"crossref","first-page":"1321","DOI":"10.3382\/ps\/pey456","article-title":"A mono-component microbial protease improves performance, net energy, and digestibility of amino acids and starch, and upregulates jejunal expression of genes responsible for peptide transport in broilers fed corn\/wheat-based diets supplemented with xylanase and phytase","volume":"98","author":"Cowieson","year":"2019","journal-title":"Poult. Sci"},{"key":"2024060513273077100_CIT0009","doi-asserted-by":"crossref","first-page":"925","DOI":"10.1093\/ps\/83.6.925","article-title":"Influence of whole wheat and xylanase on broiler performance and microbial composition and activity in the digestive tract","volume":"83","author":"Engberg","year":"2004","journal-title":"Poult. Sci"},{"key":"2024060513273077100_CIT0010","doi-asserted-by":"crossref","first-page":"1975","DOI":"10.3382\/ps.2010-01264","article-title":"Effects of xylanase and citric acid on the performance, nutrient retention, and characteristics of gastrointestinal tract of broilers fed low-phosphorus wheat-based diets","volume":"90","author":"Esmaeilipour","year":"2011","journal-title":"Poult. Sci"},{"key":"2024060513273077100_CIT0011","doi-asserted-by":"crossref","first-page":"489","DOI":"10.1038\/nrd2589","article-title":"Fatty acid-binding proteins: role in metabolic diseases and potential as drug targets","volume":"7","author":"Furuhashi","year":"2008","journal-title":"Nat. Rev. Drug Discov"},{"key":"2024060513273077100_CIT0012","doi-asserted-by":"crossref","first-page":"1663","DOI":"10.3382\/ps.2010-00801","article-title":"Dietary protein composition influences abundance of peptide and amino acid transporter messenger ribonucleic acid in the small intestine of 2 lines of broiler chicks","volume":"89","author":"Gilbert","year":"2010","journal-title":"Poult. Sci"},{"key":"2024060513273077100_CIT0013","doi-asserted-by":"crossref","first-page":"204","DOI":"10.1111\/jpn.13231","article-title":"Influence of exogenous acid protease in broiler chickens fed corn-soybean meal-based diets","volume":"104","author":"Hu","year":"2020","journal-title":"J. Anim. Physiol. Anim. Nutr. (Berl)"},{"key":"2024060513273077100_CIT0014","doi-asserted-by":"crossref","first-page":"644325","DOI":"10.3389\/fvets.2021.644325","article-title":"Impact of fermented or enzymatically fermented dried olive pomace on growth, expression of digestive enzyme and glucose transporter genes, oxidative stability of frozen meat, and economic efficiency of broiler chickens","volume":"8","author":"Ibrahim","year":"2021","journal-title":"Front Vet Sci"},{"key":"2024060513273077100_CIT0015","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1016\/S0377-8401(00)00223-6","article-title":"Intestinal development and body growth of broiler chicks on diets supplemented with non-starch polysaccharides","volume":"89","author":"Iji","year":"2001","journal-title":"Anim. Feed Sci. Technol"},{"key":"2024060513273077100_CIT0016","doi-asserted-by":"crossref","first-page":"1365","DOI":"10.5713\/ab.20.0663","article-title":"Synergistic effect of exogenous multi-enzyme and phytase on growth performance, nutrients digestibility, blood metabolites, intestinal microflora and morphology in broilers fed corn-wheat-soybean meal diets","volume":"34","author":"Kim","year":"2021","journal-title":"Anim. Biosci"},{"key":"2024060513273077100_CIT0017","doi-asserted-by":"crossref","first-page":"1561","DOI":"10.3382\/ps.0681561","article-title":"Influence of age on lipase, amylase, and protease activities in pancreatic tissue and intestinal contents of young turkeys","volume":"68","author":"Krogdahl","year":"1989","journal-title":"Poult. Sci"},{"key":"2024060513273077100_CIT0018","doi-asserted-by":"crossref","first-page":"2078","DOI":"10.3382\/ps\/pey042","article-title":"Meta-analysis: explicit value of mono-component proteases in monogastric diets","volume":"97","author":"Lee","year":"2018","journal-title":"Poult. Sci"},{"key":"2024060513273077100_CIT0019","doi-asserted-by":"crossref","first-page":"144","DOI":"10.1111\/jpn.13211","article-title":"Age-related intestinal monosaccharides transporters expression and villus surface area increase in broiler and layer chickens","volume":"104","author":"Li","year":"2020","journal-title":"J. Anim. Physiol. Anim. Nutr. (Berl)"},{"key":"2024060513273077100_CIT0021","doi-asserted-by":"crossref","first-page":"566","DOI":"10.3382\/ps\/pew258","article-title":"Effects of dietary xylanase supplementation on performance and functional digestive parameters in broilers fed wheat-based diets","volume":"96","author":"Liu","year":"2017","journal-title":"Poult. Sci"},{"key":"2024060513273077100_CIT0020","doi-asserted-by":"crossref","first-page":"109177","DOI":"10.1016\/j.meatsci.2023.109177","article-title":"Fermented mixed feed regulates intestinal microbial community and metabolism and alters pork flavor and umami","volume":"201","author":"Liu","year":"2023","journal-title":"Meat Sci"},{"key":"2024060513273077100_CIT0022","volume-title":"Feeding standard of chicken (NY\/T 33-2004)","author":"MAP, M. o. A. o. P. R. C","year":"2004"},{"key":"2024060513273077100_CIT0023","doi-asserted-by":"crossref","first-page":"101846","DOI":"10.1016\/j.psj.2022.101846","article-title":"Non-starch polysaccharide degradation in the gastrointestinal tract of broiler chickens fed commercial-type diets supplemented with either a single dose of xylanase, a double dose of xylanase, or a cocktail of non-starch polysaccharide-degrading enzymes","volume":"101","author":"Morgan","year":"2022","journal-title":"Poult. Sci"},{"key":"2024060513273077100_CIT0024","volume-title":"Nutrient requirments of poultry","author":"NRC","year":"1994"},{"key":"2024060513273077100_CIT0025","doi-asserted-by":"crossref","first-page":"353","DOI":"10.1007\/s00726-017-2510-6","article-title":"Intestinal amino acid and peptide transporters in broiler are modulated by dietary amino acids and protein","volume":"50","author":"Osmanyan","year":"2018","journal-title":"Amino Acids"},{"key":"2024060513273077100_CIT0026","doi-asserted-by":"crossref","first-page":"2854","DOI":"10.3382\/ps\/pey151","article-title":"Effects of a protease and essential oils on growth performance, blood cell profiles, nutrient retention, ileal microbiota, excreta gas emission, and breast meat quality in broiler chicks","volume":"97","author":"Park","year":"2018","journal-title":"Poult. Sci"},{"key":"2024060513273077100_CIT0027","doi-asserted-by":"crossref","first-page":"463","DOI":"10.3382\/ps\/pez533","article-title":"Effects of dietary Achyranthes japonica extract supplementation on the growth performance, total tract digestibility, cecal microflora, excreta noxious gas emission, and meat quality of broiler chickens","volume":"99","author":"Park","year":"2020","journal-title":"Poult. Sci"},{"key":"2024060513273077100_CIT0028","doi-asserted-by":"crossref","first-page":"2451","DOI":"10.3382\/ps\/pey093","article-title":"Effect of dietary Spirulina (Arthrospira) platensis on the growth performance, antioxidant enzyme activity, nutrient digestibility, cecal microflora, excreta noxious gas emission, and breast meat quality of broiler chickens","volume":"97","author":"Park","year":"2018","journal-title":"Poult. Sci"},{"key":"2024060513273077100_CIT0029","doi-asserted-by":"crossref","first-page":"594","DOI":"10.1080\/00071660701615788","article-title":"Influence of wheat-based diets supplemented with xylanase, bile acid and antibiotics on performance, digestive tract measurements and gut morphology of broilers compared with a maize-based diet","volume":"48","author":"Parsaie","year":"2007","journal-title":"Br. Poult. Sci"},{"key":"2024060513273077100_CIT0030","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1016\/j.aninu.2017.12.001","article-title":"Influence of dietary carbohydrases, individually or in combination with phytase or an acidifier, on performance, gut morphology and microbial population in broiler chickens fed a wheat-based diet","volume":"5","author":"Roofchaei","year":"2019","journal-title":"Anim Nutr"},{"key":"2024060513273077100_CIT0032","doi-asserted-by":"crossref","first-page":"602","DOI":"10.1080\/00071660500255661","article-title":"A mixture of pure cellulase, hemicellulase and pectinase improves broiler performance","volume":"46","author":"Saleh","year":"2005","journal-title":"Br. Poult. Sci"},{"key":"2024060513273077100_CIT0031","doi-asserted-by":"crossref","first-page":"e0198085","DOI":"10.1371\/journal.pone.0198085","article-title":"Exogenous dietary enzyme formulations improve growth performance of broiler chickens fed a low-energy diet targeting the intestinal nutrient transporter genes","volume":"13","author":"Saleh","year":"2018","journal-title":"PLoS One"},{"key":"2024060513273077100_CIT0033","doi-asserted-by":"crossref","first-page":"647","DOI":"10.1111\/j.1439-0396.2008.00852.x","article-title":"Intestinal function and gut microflora of broiler chickens as influenced by cereal grains and microbial enzyme supplementation","volume":"93","author":"Shakouri","year":"2009","journal-title":"J. Anim. Physiol. Anim. Nutr. (Berl)"},{"key":"2024060513273077100_CIT0034","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1017\/S0043933915000136","article-title":"\u00df-Mannan and mannanase in poultry nutrition","volume":"71","author":"Shastak","year":"2015","journal-title":"World\u2019s Poultry Sci. J"},{"key":"2024060513273077100_CIT0035","doi-asserted-by":"crossref","first-page":"3733","DOI":"10.1007\/s13197-021-05262-7","article-title":"Maltooligosaccharide forming amylases and their applications in food and pharma industry","volume":"59","author":"Shinde","year":"2022","journal-title":"J. Food Sci. Technol"},{"key":"2024060513273077100_CIT0036","doi-asserted-by":"crossref","first-page":"2013","DOI":"10.3382\/ps.2011-01372","article-title":"Recent advances in research on enzymes for poultry diets","volume":"90","author":"Slominski","year":"2011","journal-title":"Poult. Sci"},{"key":"2024060513273077100_CIT0037","doi-asserted-by":"crossref","first-page":"5862","DOI":"10.3382\/ps\/pez290","article-title":"Utilization of corn-based diets supplemented with an exogenous \u03b1-amylase for broilers","volume":"98","author":"Stefanello","year":"2019","journal-title":"Poult. Sci"},{"key":"2024060513273077100_CIT0038","doi-asserted-by":"crossref","first-page":"500","DOI":"10.2527\/jas.2007-0536","article-title":"A comparison of methods of fitting several models to nutritional response data","volume":"86","author":"Vedenov","year":"2008","journal-title":"J. Anim. Sci"},{"key":"2024060513273077100_CIT0039","doi-asserted-by":"crossref","first-page":"1733","DOI":"10.5713\/ab.21.0534","article-title":"Efficacy of combination of endo-xylanase and xylan-debranching enzymes in improving cereal bran utilization in piglet diet","volume":"35","author":"Wang","year":"2022","journal-title":"Anim Biosci"},{"key":"2024060513273077100_CIT0040","doi-asserted-by":"crossref","first-page":"1328","DOI":"10.5187\/jast.2021.e104","article-title":"Multi-carbohydrase application into energy and amino acid deficient broiler diets: A strategy to enhance performance of broiler chickens","volume":"63","author":"Wickramasuriya","year":"2021","journal-title":"J. Anim. Sci. Technol"},{"key":"2024060513273077100_CIT0041","doi-asserted-by":"crossref","first-page":"102268","DOI":"10.1016\/j.psj.2022.102268","article-title":"Effect of emulsifier and multicarbohydrase enzyme supplementation on performance and nutrient digestibility in broiler diets containing rapeseed meal","volume":"102","author":"Wi\u015bniewska","year":"2023","journal-title":"Poult. Sci"},{"key":"2024060513273077100_CIT0042","doi-asserted-by":"crossref","first-page":"210","DOI":"10.1080\/00071660701252988","article-title":"Effect of cellulase supplementation in low-crude protein diets on performance, nitrogen excretion, fat deposition, hepatic lipogenic and lipolytic enzyme activity in broilers","volume":"48","author":"Yamazaki","year":"2007","journal-title":"Br. Poult. Sci"},{"key":"2024060513273077100_CIT0043","doi-asserted-by":"crossref","first-page":"9297","DOI":"10.1021\/acs.jafc.7b02746","article-title":"Effects of long-term protein restriction on meat quality, muscle amino acids, and amino acid transporters in pigs","volume":"65","author":"Yin","year":"2017","journal-title":"J. Agric. Food Chem"},{"key":"2024060513273077100_CIT0045","doi-asserted-by":"crossref","first-page":"e0173941","DOI":"10.1371\/journal.pone.0173941","article-title":"Effects of protease and non-starch polysaccharide enzyme on performance, digestive function, activity and gene expression of endogenous enzyme of broilers","volume":"12","author":"Yuan","year":"2017","journal-title":"PLoS One"},{"key":"2024060513273077100_CIT0044","doi-asserted-by":"crossref","first-page":"2102","DOI":"10.1080\/1828051X.2021.2000895","article-title":"Effects of dietary phytosterols supplementation on serum parameters, nutrient digestibility and digestive enzyme of white feather broilers","volume":"20","author":"Yuan","year":"2021","journal-title":"Ital. J. Anim. Sci"}],"container-title":["Journal of Animal Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/jas\/advance-article-pdf\/doi\/10.1093\/jas\/skae133\/57520357\/skae133.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jas\/article-pdf\/doi\/10.1093\/jas\/skae133\/58104998\/skae133.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jas\/article-pdf\/doi\/10.1093\/jas\/skae133\/58104998\/skae133.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,5]],"date-time":"2024-06-05T17:49:14Z","timestamp":1717609754000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jas\/article\/doi\/10.1093\/jas\/skae133\/7668624"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,1,1]]},"references-count":45,"URL":"https:\/\/doi.org\/10.1093\/jas\/skae133","relation":{},"ISSN":["0021-8812","1525-3163"],"issn-type":[{"value":"0021-8812","type":"print"},{"value":"1525-3163","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2024,1,1]]},"published":{"date-parts":[[2024,1,1]]},"article-number":"skae133"}}