{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T22:53:34Z","timestamp":1790722414106,"version":"4.1.0"},"reference-count":64,"publisher":"Oxford University Press (OUP)","license":[{"start":{"date-parts":[[2024,1,1]],"date-time":"2024-01-01T00:00:00Z","timestamp":1704067200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,1,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Mastitis is an important disease with economic and welfare implications in both clinical and subclinical states. The aim of this research was to sequence the hypervariable V4 region of the 16S rRNA gene to describe the microbial diversity and taxonomy of milk from clinically healthy ewes (Rambouillet, WF\u2005=\u20059; Hampshire, BF\u2005=\u20055). Experimental ewes represented a subset of a larger study assessing the impacts of divergent dietary zinc (Zn) concentrations [1\u2005\u00d7\u2005National Academics of Sciences, Engineering, and Medicine (NASEM) recommendations\u2005=\u2005CON or 3\u2005\u00d7\u2005NASEM recommendations\u2005=\u2005ZnTRT] throughout late gestation and lactation. Milk was collected at four periods during early lactation (18 to 24\u00a0h, 7 d, 14 d, and 21 d postpartum) and at weaning (84\u2005\u00b1\u200514 d postpartum). Somatic cell counts (SCC) were quantified, averaged, and classed (low:\u2005&amp;lt;\u2005500\u2005\u00d7\u2005103; medium: 500\u2005\u00d7\u2005103 \u2013 100\u2005\u00d7\u2005104; high:\u2005&amp;gt;\u2005100\u2005\u00d7\u2005104 cells\/mL). Milk samples (n\u2005=\u200567) were sequenced to identify bacteria and archaea; the most abundant phyla were Actinobacteria, Bacteroidetes, Cyanobacteria, Euryarchaeota, Firmicutes, Fusobacteria, Lentisphaerae, Proteobacteria, Spirochaetes, Tenericutes, Saccharibacteria TM7, and Verrucomicrobia. Mastitis pathogens were among the most relatively abundant genera, including Staphylococcus, Mannheimia, Corynebacterium, and Pseudomonas. Effects of breed, dietary Zn concentration, SCC class, and their two-way interactions on milk microbiome diversity and taxonomy were assessed within early lactation (using a repeated measures model) and weaning samples. Alpha-diversity metrics included Pielou\u2019s evenness, Faith\u2019s phylogenetic diversity, and Shannon\u2019s entropy indices. The main and interactive effects between Zn treatment, breed, SCC class, and period were variable in early lactation and not evident in weaning samples. Milk from BF ewes had increased Faith\u2019s phylogenetic diversity and Shannon\u2019s entropy, and differed in unweighted UniFrac composition (P\u2005\u2264\u20050.10). Milk from CON ewes had a reduced rate of composition change through early lactation (P\u2005=\u20050.02) indicating greater microbiome stability than ZnTRT ewe milk. These results support that milk is not sterile, and breed, dietary Zn concentration, and SCC class variably affect the milk microbiome. Findings from the current study provide important foundational insights into the effects of increased dietary Zn supplementation on longitudinal changes in the milk microbiome and associations with mammary gland health and mastitis.<\/jats:p>","DOI":"10.1093\/jas\/skae163","type":"journal-article","created":{"date-parts":[[2024,6,11]],"date-time":"2024-06-11T19:42:41Z","timestamp":1718134961000},"source":"Crossref","is-referenced-by-count":2,"title":["Milk microbiome in the first month of lactation and at weaning from ewes supplemented with zinc pre- and postpartum"],"prefix":"10.1093","volume":"102","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8046-8310","authenticated-orcid":false,"given":"Ryan M","family":"Knuth","sequence":"first","affiliation":[{"name":"University of Wyoming Department of Animal Science, , Laramie, WY 82071 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chad M","family":"Page","sequence":"additional","affiliation":[{"name":"University of Wyoming Department of Animal Science, , Laramie, WY 82071 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1928-0359","authenticated-orcid":false,"given":"Whitney C","family":"Stewart","sequence":"additional","affiliation":[{"name":"University of Wyoming Department of Animal Science, , Laramie, WY 82071 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3276-5190","authenticated-orcid":false,"given":"Gwendolynn L","family":"Hummel","sequence":"additional","affiliation":[{"name":"University of Wyoming Department of Animal Science, , Laramie, WY 82071 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kelly L","family":"Woodruff","sequence":"additional","affiliation":[{"name":"University of Wyoming Department of Animal Science, , Laramie, WY 82071 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jaelyn R","family":"Whaley","sequence":"additional","affiliation":[{"name":"University of Wyoming Department of Animal Science, , Laramie, WY 82071 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexis L","family":"Springer","sequence":"additional","affiliation":[{"name":"University of Wyoming Department of Animal Science, , Laramie, WY 82071 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kathleen J","family":"Austin","sequence":"additional","affiliation":[{"name":"University of Wyoming Department of Animal Science, , Laramie, WY 82071 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3527-8814","authenticated-orcid":false,"given":"Thomas W","family":"Murphy","sequence":"additional","affiliation":[{"name":"USDA, ARS, Livestock Bio-Systems Research Unit, Roman. 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