{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,20]],"date-time":"2026-06-20T15:04:08Z","timestamp":1781967848952,"version":"3.54.5"},"reference-count":26,"publisher":"Oxford University Press (OUP)","license":[{"start":{"date-parts":[[2024,6,27]],"date-time":"2024-06-27T00:00:00Z","timestamp":1719446400000},"content-version":"vor","delay-in-days":178,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,1,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Our objective was to determine if the addition of a concentrated human recombinant transforming growth factor beta-1 (TGF) to bovine semen at the time of AI would result in increased risk of pregnancy in beef and dairy cows. Suckled beef cows (n\u2005=\u20051,132) in 11 herds across 2 states and lactating dairy cows (n\u2005=\u20052,208) in one organic-certified herd were enrolled. Beef cows received fixed-time AI (FTAI) following a 7 d CO-Synch\u2005+\u2005controlled internal drug release estrous synchronization protocol. Dairy cows were inseminated following observation of natural estrus expression. Cows received either no treatment as a control (CON) or 10\u00a0ng of TGF in 10 \u03bcL added through the cut-end of a thawed straw of semen immediately prior to AI. At the time of FTAI of beef cows, the mean\u2005\u00b1\u2005SD age was 5.0\u2005\u00b1\u20052.4 yr, BCS was 5.3\u2005\u00b1\u20050.7, and days postpartum was 78.2\u2005\u00b1\u200515.5 d. The overall pregnancy risk (PR) in beef cows was 55.2% to AI and 90.5% season-long. PR in beef cows was not affected (P\u2005=\u20050.27) by the addition of TGF (53.1% vs. 58.1%). Furthermore, there was no difference (P\u2005=\u20050.88) for season-long PR in beef cows that received TGF (91.2% vs. 91.5%). At the time of insemination of dairy cows, the mean\u2005\u00b1\u2005SD lactation was 3.0\u2005\u00b1\u20051.3 lactations, BCS was 2.9\u2005\u00b1\u20050.3, days in milk was 115.6\u2005\u00b1\u200556.6 d, and cows had received 2.4\u2005\u00b1\u20051.5 inseminations\/cow. The overall pregnancy risk to AI in dairy cows was 23.1%. PR to AI for dairy cows was not affected (P\u2005=\u20050.32) by addition of TGF (22.0% vs. 23.8%). In conclusion, PR to AI was not affected by addition of TGF to thawed semen immediately prior to AI in beef or dairy cows.<\/jats:p>","DOI":"10.1093\/jas\/skae169","type":"journal-article","created":{"date-parts":[[2024,6,27]],"date-time":"2024-06-27T11:16:52Z","timestamp":1719487012000},"source":"Crossref","is-referenced-by-count":4,"title":["Pregnancy risk in beef and dairy cows after supplementing semen with transforming growth factor beta-1 at the time of artificial insemination"],"prefix":"10.1093","volume":"102","author":[{"given":"Katelyn L M","family":"Fritsche","sequence":"first","affiliation":[{"name":"Department of Animal Sciences, Colorado State University , Fort Collins, CO 80523 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jason K","family":"Ahola","sequence":"additional","affiliation":[{"name":"Department of Animal Sciences, Colorado State University , Fort Collins, CO 80523 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7111-3377","authenticated-orcid":false,"given":"Pablo J","family":"Pinedo","sequence":"additional","affiliation":[{"name":"Department of Animal Sciences, Colorado State University , Fort Collins, CO 80523 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"George E","family":"Seidel","sequence":"additional","affiliation":[{"name":"Animal Reproduction and Biotechnology Laboratory, Colorado State University , Fort Collins, CO 80523 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ryan D","family":"Rhoades","sequence":"additional","affiliation":[{"name":"Department of Animal Sciences, Colorado State University , Fort Collins, CO 80523 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeffrey S","family":"Stevenson","sequence":"additional","affiliation":[{"name":"Department of Animal Sciences and Industry, Kansas State University , Manhattan, KS 66506 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K C","family":"Olson","sequence":"additional","affiliation":[{"name":"Department of Animal Sciences and Industry, Kansas State University , Manhattan, KS 66506 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John R","family":"Jaeger","sequence":"additional","affiliation":[{"name":"Department of Animal Sciences and Industry, Kansas State University , Manhattan, KS 66506 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David M","family":"Grieger","sequence":"additional","affiliation":[{"name":"Department of Animal Sciences and Industry, Kansas State University , Manhattan, KS 66506 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5438-2137","authenticated-orcid":false,"given":"John J","family":"Bromfield","sequence":"additional","affiliation":[{"name":"Department of Animal Sciences, University of Florida , Gainesville, FL 32611 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2024,6,27]]},"reference":[{"key":"2024071310004629000_CIT0001","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1016\/j.anireprosci.2008.10.015","article-title":"Species-specific interaction of seminal plasma on sperm-neutrophil binding","volume":"114","author":"Alghamdi","year":"2009","journal-title":"Anim. 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