{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T21:56:39Z","timestamp":1790805399860,"version":"4.1.0"},"reference-count":32,"publisher":"Oxford University Press (OUP)","license":[{"start":{"date-parts":[[2024,7,20]],"date-time":"2024-07-20T00:00:00Z","timestamp":1721433600000},"content-version":"vor","delay-in-days":201,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,1,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Recent study showed that zinc (Zn) and amino acid transporters may be involved in enhancing Zn absorption from Zn proteinate with moderate chelation strength (Zn-Prot M) in the duodenum of broilers. However, the specific mechanisms by which Zn-Prot M promotes the above Zn absorption are unknown. Therefore, in this study, 3 experiments were conducted to investigate specific and direct effects of Zn-Prot M and Zn sulfate (ZnS) on Zn absorption and expression of related transporters in primary duodenal epithelial cells of broiler embryos so as to preliminarily address possible mechanisms. In experiment 1, cells were treated with 100 \u03bcmol Zn\/L as ZnS or Zn-Prot M for 20, 40, 60, 80, 100, or 120\u00a0min. Experiment 2 consisted of 3 sub-experiments. In experiment 2A, cells were treated with a Zn-unsupplemented basal medium (Control) or the basal medium supplemented with 100 or 200 \u03bcmol Zn\/L as ZnS or Zn-Prot M for 60\u00a0min; in experiment 2B, cells were treated with a Zn-unsupplemented basal medium (Control) or the basal medium supplemented with 200 \u03bcmol Zn\/L of as the ZnS or Zn-Prot M for 120\u00a0min; in experiment 2C, cells were treated with a Zn-unsupplemented basal medium (Control) or the basal medium supplemented with 400 or 800 \u03bcmol Zn\/L as ZnS or Zn-Prot M for 120\u00a0min. In experiment 3, cells were treated with a Zn-unsupplemented basal medium (Control) or the basal medium supplemented with 400 \u03bcmol Zn\/L as ZnS or Zn-Prot M for 120\u00a0min. The results of experiment 1 indicated that the minimum incubation time for saturable Zn absorption was determined to be 50.83\u00a0min using the best fit line. The results in experiment 2 demonstrated that a Zn concentration of 400 \u03bcmol\/L and an incubation time of 120\u00a0min were suitable to increase the absorption of Zn from Zn-Prot M compared to ZnS. In experiment 3, Zn absorption across cell monolayers was significantly increased by Zn addition (P\u2005&amp;lt;\u20050.05), and was significantly greater with Zn-Prot M than with ZnS (P\u2005&amp;lt;\u20050.05). Compared to the control, Zn addition significantly decreased Zn transporter 10 and peptide-transporter 1 mRNA expression levels and increased y\u2005+\u2005L-type amino transporter 2 (y\u2005+\u2005LAT2) protein abundance (P\u2005&amp;lt;\u20050.05). Moreover, protein expression levels of zrt\/irt-like protein 3 (ZIP3), zrt\u2013irt-like protein 5 (ZIP5), and y\u2005+\u2005LAT2 were significantly greater for Zn-Prot M than for ZnS (P\u2005&amp;lt;\u20050.05). These findings suggest that Zn-Prot M promote Zn absorption by increasing ZIP3, ZIP5 and y\u2005+\u2005LAT2 protein expression levels in primary duodenal epithelial cells.<\/jats:p>","DOI":"10.1093\/jas\/skae204","type":"journal-article","created":{"date-parts":[[2024,7,20]],"date-time":"2024-07-20T14:11:42Z","timestamp":1721484702000},"source":"Crossref","is-referenced-by-count":9,"title":["Zinc proteinate with moderate chelation strength enhances zinc absorption by upregulating the expression of zinc and amino acid transporters in primary cultured duodenal epithelial cells of broiler embryos"],"prefix":"10.1093","volume":"102","author":[{"given":"Yun","family":"Hu","sequence":"first","affiliation":[{"name":"Poultry Mineral Nutrition Laboratory, College of Animal Science and Technology, Yangzhou University , Yangzhou , People\u2019s Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Wu","sequence":"additional","affiliation":[{"name":"Poultry Mineral Nutrition Laboratory, College of Animal Science and Technology, Yangzhou University , Yangzhou , People\u2019s Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liang","family":"Huang","sequence":"additional","affiliation":[{"name":"Mineral Nutrition Research Division, Institute of Animal Science, Chinese Academy of Agricultural Sciences , Beijing , People\u2019s Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liyang","family":"Zhang","sequence":"additional","affiliation":[{"name":"Mineral Nutrition Research Division, Institute of Animal Science, Chinese Academy of Agricultural Sciences , Beijing , People\u2019s Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunyu","family":"Cao","sequence":"additional","affiliation":[{"name":"Poultry Mineral Nutrition Laboratory, College of Animal Science and Technology, Yangzhou University , Yangzhou , People\u2019s Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weiyun","family":"Zhang","sequence":"additional","affiliation":[{"name":"Poultry Mineral Nutrition Laboratory, College of Animal Science and Technology, Yangzhou University , Yangzhou , People\u2019s Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yangyang","family":"Hu","sequence":"additional","affiliation":[{"name":"Poultry Mineral Nutrition Laboratory, College of Animal Science and Technology, Yangzhou University , Yangzhou , People\u2019s Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoyan","family":"Cui","sequence":"additional","affiliation":[{"name":"Poultry Mineral Nutrition Laboratory, College of Animal Science and Technology, Yangzhou University , Yangzhou , People\u2019s Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tingting","family":"Li","sequence":"additional","affiliation":[{"name":"Poultry Mineral Nutrition Laboratory, College of Animal Science and Technology, Yangzhou University , Yangzhou , People\u2019s Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shengchen","family":"Wang","sequence":"additional","affiliation":[{"name":"Poultry Mineral Nutrition Laboratory, College of Animal Science and Technology, Yangzhou University , Yangzhou , People\u2019s Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xugang","family":"Luo","sequence":"additional","affiliation":[{"name":"Poultry Mineral Nutrition Laboratory, College of Animal Science and Technology, Yangzhou University , Yangzhou , People\u2019s Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2024,7,20]]},"reference":[{"key":"2024083010402698600_CIT0001","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1111\/jpn.13260","article-title":"Kinetics of phosphorus absorption and expressions of related transporters in primary cultured duodenal epithelial cells of chick embryos","volume":"104","author":"Cao","year":"2020","journal-title":"J. Anim. Physiol. Anim. Nutr"},{"key":"2024083010402698600_CIT0002","doi-asserted-by":"crossref","first-page":"304","DOI":"10.1074\/mcp.M200008-MCP200","article-title":"Discordant protein and mRNA expression in lung adenocarcinomas","volume":"1","author":"Chen","year":"2002","journal-title":"Mol. Cell. Proteomics"},{"key":"2024083010402698600_CIT0003","doi-asserted-by":"crossref","first-page":"983954","DOI":"10.3389\/fphys.2022.983954","article-title":"Comparison of zinc bioavailability in zinc-glycine and zinc-methionine chelates for broilers fed with a corn-soybean meal diet","volume":"13","author":"Chen","year":"2022","journal-title":"Front. Physiol"},{"key":"2024083010402698600_CIT0004","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1002\/dvg.20211","article-title":"Mouse ZIP1 and ZIP3 genes together are essential for adaptation to dietary zinc deficiency during pregnancy","volume":"44","author":"Dufner-Beattie","year":"2006","journal-title":"Genesis"},{"key":"2024083010402698600_CIT0005","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1016\/j.lfs.2014.05.021","article-title":"Amino acid facilitates absorption of copper in the Caco-2 cell culture model","volume":"109","author":"Gao","year":"2014","journal-title":"Life Sci"},{"key":"2024083010402698600_CIT0006","doi-asserted-by":"crossref","first-page":"e82149","DOI":"10.1371\/journal.pone.0082149","article-title":"The zinc transporter Zip5 (Slc39a5) regulates intestinal zinc excretion and protects the pancreas against zinc toxicity","volume":"8","author":"Geiser","year":"2013","journal-title":"PLoS One"},{"key":"2024083010402698600_CIT0007","doi-asserted-by":"crossref","first-page":"1146","DOI":"10.3382\/ps\/pey484","article-title":"Zinc source influences the gene expression of zinc transporters in jejunum and cecal tonsils during broiler challenge with Eimeria maxima and Clostridium perfringens","volume":"98","author":"He","year":"2019","journal-title":"Poult. Sci"},{"key":"2024083010402698600_CIT0008","doi-asserted-by":"crossref","first-page":"952941","DOI":"10.3389\/fphys.2022.952941","article-title":"Organic zinc with moderate chelation strength enhances zinc absorption in the small intestine and expression of related transporters in the duodenum of broilers","volume":"13","author":"Hu","year":"2022","journal-title":"Front. Physiol"},{"key":"2024083010402698600_CIT0009","doi-asserted-by":"crossref","first-page":"102477","DOI":"10.1016\/j.psj.2023.102477","article-title":"The organic zinc with moderate chelation strength enhances the expression of related transporters in the jejunum and ileum of broilers","volume":"102","author":"Hu","year":"2023","journal-title":"Poult. Sci"},{"key":"2024083010402698600_CIT0010","doi-asserted-by":"crossref","first-page":"292","DOI":"10.1038\/ng1197-292","article-title":"A novel gene involved in zinc transport is deficient in the lethal milk mouse","volume":"17","author":"Huang","year":"1997","journal-title":"Nat. Genet"},{"key":"2024083010402698600_CIT0011","doi-asserted-by":"crossref","first-page":"2038","DOI":"10.2527\/jas.2008-1212","article-title":"Relative bioavailabilities of organic zinc sources with different chelation strengths for broilers fed a conventional corn-soybean meal diet","volume":"87","author":"Huang","year":"2009","journal-title":"J. Anim. Sci"},{"key":"2024083010402698600_CIT0012","doi-asserted-by":"crossref","first-page":"103696","DOI":"10.1016\/j.psj.2024.103696","article-title":"Zinc alleviates thermal stress-induced damage to the integrity and barrier function of cultured chicken embryonic primary jejunal epithelial cells via the MAPK and PI3K\/AKT\/mTOR signaling pathways","volume":"103","author":"Huang","year":"2024","journal-title":"Poult. Sci"},{"key":"2024083010402698600_CIT0013","doi-asserted-by":"crossref","first-page":"92","DOI":"10.1265\/jjh.68.92","article-title":"Overview of and update on the physiological functions of mammalian zinc transporters","volume":"68","author":"Kambe","year":"2013","journal-title":"Nihon Eiseigaku Zasshi"},{"key":"2024083010402698600_CIT0014","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1080\/01652176.2023.2298491","article-title":"Effects of zinc supplementation from organic and inorganic sources on growth, blood biochemical indices, and intestinal microarchitecture in broilers","volume":"44","author":"Khan","year":"2024","journal-title":"Vet. Q"},{"key":"2024083010402698600_CIT0015","doi-asserted-by":"crossref","first-page":"336","DOI":"10.3390\/ijms17030336","article-title":"The Functions of metallothionein and ZIP and ZnT transporters: an overview and perspective","volume":"17","author":"Kimura","year":"2016","journal-title":"Int. J. Mol. Sci"},{"key":"2024083010402698600_CIT0016","doi-asserted-by":"crossref","first-page":"941","DOI":"10.1007\/s10534-013-9670-y","article-title":"Kinetics of manganese transport and gene expressions of manganese transport carriers in Caco-2 cell monolayers","volume":"26","author":"Li","year":"2013","journal-title":"Biometals"},{"key":"2024083010402698600_CIT0017","doi-asserted-by":"crossref","first-page":"621","DOI":"10.1016\/j.aninu.2021.01.003","article-title":"Zinc alleviates the heat stress of primary cultured hepatocytes of broiler embryos via enhancing the antioxidant ability and attenuating the heat shock responses","volume":"7","author":"Li","year":"2021","journal-title":"Anim. Nutr"},{"key":"2024083010402698600_CIT0018","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method","volume":"25","author":"Livak","year":"2001","journal-title":"Methods"},{"key":"2024083010402698600_CIT0019","doi-asserted-by":"crossref","first-page":"366","DOI":"10.3382\/ps\/pey386","article-title":"Effects of organic zinc on tibia quality, mineral deposit, and metallothionein expression level of aged hens","volume":"98","author":"Min","year":"2019","journal-title":"Poult. Sci"},{"key":"2024083010402698600_CIT0020","first-page":"V11120654","article-title":"Zinc is the most important trace element","volume":"176","author":"Muhamed","year":"2014","journal-title":"Ugeskr. Laeger"},{"key":"2024083010402698600_CIT0021","doi-asserted-by":"crossref","first-page":"1418","DOI":"10.1007\/s12011-022-03223-5","article-title":"Potentials of dietary zinc supplementation in improving growth performance, health status, and meat quality of broiler chickens","volume":"201","author":"Ogbuewu","year":"2023","journal-title":"Biol. Trace Elem. Res"},{"key":"2024083010402698600_CIT0022","doi-asserted-by":"crossref","first-page":"5774","DOI":"10.1007\/s12011-023-03614-2","article-title":"Production and evaluation of encapsulated zinc oxide on performance, ileal digestibility and zinc transporter gene expression in broiler chicken","volume":"201","author":"Rao","year":"2023","journal-title":"Biol. Trace Elem. Res"},{"key":"2024083010402698600_CIT0023","first-page":"768","article-title":"Zinc: an essential micronutrient","volume":"79","author":"Saper","year":"2009","journal-title":"Am. Fam. Physician"},{"key":"2024083010402698600_CIT0024","doi-asserted-by":"crossref","first-page":"337","DOI":"10.2119\/2007-00037.Sekler","article-title":"Mechanism and regulation of cellular zinc transport","volume":"13","author":"Sekler","year":"2007","journal-title":"Mol. Med"},{"key":"2024083010402698600_CIT0025","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1016\/j.jnutbio.2017.01.013","article-title":"Zinc enhances intestinal epithelial barrier function through the PI3K\/AKT\/mTOR signaling pathway in Caco-2 cells","volume":"43","author":"Shao","year":"2017","journal-title":"J. Nutr. Biochem"},{"key":"2024083010402698600_CIT0026","doi-asserted-by":"crossref","first-page":"620","DOI":"10.2527\/jas.2014-8165","article-title":"Effect of zinc-bearing zeolite clinoptilolite on growth performance, zinc accumulation, and gene expression of zinc transporters in broilers","volume":"93","author":"Tang","year":"2015","journal-title":"J. Anim. Sci"},{"key":"2024083010402698600_CIT0027","doi-asserted-by":"crossref","first-page":"5647","DOI":"10.1021\/bi00476a001","article-title":"Zinc coordination, function, and structure of zinc enzymes and other proteins","volume":"29","author":"Vallee","year":"1990","journal-title":"Biochemistry"},{"key":"2024083010402698600_CIT0028","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1007\/s12011-019-01921-1","article-title":"The systemic zinc homeostasis was modulated in broilers challenged by salmonella","volume":"196","author":"Wu","year":"2020","journal-title":"Biol. Trace Elem. Res"},{"key":"2024083010402698600_CIT0029","doi-asserted-by":"crossref","first-page":"7627","DOI":"10.1080\/10408398.2022.2048292","article-title":"Zinc homeostasis and regulation: Zinc transmembrane transport through transporters","volume":"63","author":"Yin","year":"2023","journal-title":"Crit. Rev. Food Sci. Nutr"},{"key":"2024083010402698600_CIT0030","doi-asserted-by":"crossref","first-page":"1146","DOI":"10.3382\/ps.2007-00430","article-title":"Kinetics of zinc absorption by in situ ligated intestinal loops of broilers involved in zinc transporters","volume":"87","author":"Yu","year":"2008","journal-title":"Poult. Sci"},{"key":"2024083010402698600_CIT0031","doi-asserted-by":"crossref","first-page":"1086","DOI":"10.1017\/S0007114517001040","article-title":"Organic zinc absorption by the intestine of broilers in vivo","volume":"117","author":"Yu","year":"2017","journal-title":"Br. J. Nutr"},{"key":"2024083010402698600_CIT0032","first-page":"3159","article-title":"Isolation and identification of duodenal epithelial cells of broiler embryos in vitro and its establishment and evaluation of primary cultured absorption model","volume":"30","author":"Zhang","year":"2018","journal-title":"Chin. J. Anim. Nutr"}],"container-title":["Journal of Animal Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/jas\/advance-article-pdf\/doi\/10.1093\/jas\/skae204\/58603839\/skae204.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jas\/article-pdf\/doi\/10.1093\/jas\/skae204\/58970655\/skae204.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jas\/article-pdf\/doi\/10.1093\/jas\/skae204\/58970655\/skae204.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,8,30]],"date-time":"2024-08-30T13:36:53Z","timestamp":1725025013000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jas\/article\/doi\/10.1093\/jas\/skae204\/7717589"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024]]},"references-count":32,"URL":"https:\/\/doi.org\/10.1093\/jas\/skae204","relation":{},"ISSN":["0021-8812","1525-3163"],"issn-type":[{"value":"0021-8812","type":"print"},{"value":"1525-3163","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2024]]},"published":{"date-parts":[[2024]]},"article-number":"skae204"}}