{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T07:33:57Z","timestamp":1788852837069,"version":"build-2803163510"},"reference-count":52,"publisher":"Oxford University Press (OUP)","license":[{"start":{"date-parts":[[2024,7,24]],"date-time":"2024-07-24T00:00:00Z","timestamp":1721779200000},"content-version":"vor","delay-in-days":205,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,1,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Each year in Australia, 53% of lamb mortalities are attributed to dystocia, with subclinical maternal calcium deficiencies likely contributing to dystocia rates. A negative dietary cation and anion difference (DCAD) diet has increased circulating calcium in sheep. Therefore, this study aimed to investigate the effects of supplementing twin-bearing, grazing ewes with a negative DCAD partial mixed ration (PMR) during late gestation on ewe calcium and magnesium concentrations and subsequent lamb growth and survival. On day 120 of gestation (dG), blood samples were collected from 115 twin-bearing Merino ewes and analyzed for glucose, ketone bodies, pH, ionized calcium, and serum calcium and magnesium. On dG 130, ewes were moved into lambing paddocks and placed in the following 2 treatment groups; ewes receiving a positive DCAD PMR (DCAD\u2005=\u2005287 mEq\/kg DM; n\u2005=\u200558) and ewes receiving a negative DCAD PMR (DCAD = \u2212125 mEq\/kg DM; n\u2005=\u200557) fed as a PMR. On dG 140, a blood and urine sample were collected. The urine was tested for pH. Pasture samples were taken on dG 133 and 149 and tested for DCAD and mineral content. When a lamb was 6 to 18\u00a0h old, survival, vigor score, liveweight (LW), rectal temperature, blood glucose, and body morphology were recorded. At 10 d of age, lamb LW and survival were recorded and a milk sample was collected from ewes. At 44 d of age, lamb LW and survival were recorded. The DCAD of the pastures across the 6 paddocks ranged from 598 to 893 mEq\/kg DM. There were no differences in lamb survival, weight, or viability at any timepoint (P\u2005&amp;gt;\u20050.05). There were no differences in mineral status, metabolic state, or acid\u2013base balance between the positive and negative DCAD-supplemented ewes (P\u2005&amp;gt;\u20050.05) during supplementation (dG 140). Supplementing a negative DCAD diet to ewes grazing pasture during late gestation did not improve lamb survival. The blood and urine pH of the negative DCAD-supplemented ewes indicated a mild metabolic acidosis was not reached due to the high DCAD of the pastures. Further research needs to take careful consideration of the DCAD of pasture when designing a negative DCAD supplement in order for it to be effective.<\/jats:p>","DOI":"10.1093\/jas\/skae205","type":"journal-article","created":{"date-parts":[[2024,7,25]],"date-time":"2024-07-25T06:03:23Z","timestamp":1721887403000},"source":"Crossref","is-referenced-by-count":2,"title":["Negative dietary cation and anion difference supplementation of twin-bearing Merino ewes grazing pasture in late gestation did not affect lamb growth or survival"],"prefix":"10.1093","volume":"102","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8215-6329","authenticated-orcid":false,"given":"Amy Laurel","family":"Munn","sequence":"first","affiliation":[{"name":"Davies Livestock Research Centre, the University of Adelaide , Roseworthy, South Australia 5371, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William H E J","family":"van Wettere","sequence":"additional","affiliation":[{"name":"Davies Livestock Research Centre, the University of Adelaide , Roseworthy, South Australia 5371, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alyce Marie","family":"Swinbourne","sequence":"additional","affiliation":[{"name":"Turretfield Research Centre, South Australian Research and Development Institute , Rosedale, South Australia 5350, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ian John","family":"Lean","sequence":"additional","affiliation":[{"name":"Scibus , Camden, New South Wales 2570, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alice Caroline","family":"Weaver","sequence":"additional","affiliation":[{"name":"Turretfield Research Centre, South Australian Research and Development Institute , Rosedale, South Australia 5350, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2024,7,24]]},"reference":[{"key":"2024080807165250300_CIT0001","first-page":"41","volume-title":"Feed evaluation and diet formulation energy and protein requirements of ruminants, energy and protein requirements of ruminants: an advisory manual prepared by the AFRC Technical Committee on responses to nutrients","author":"Alderman","year":"1996"},{"key":"2024080807165250300_CIT0002","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1016\/j.livsci.2018.10.001","article-title":"Effect of calcium and magnesium supplementation on minerals profile, immune responses, and energy profile of ewes and their lambs","volume":"217","author":"Ataollahi","year":"2018","journal-title":"Livest. 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