{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T07:33:55Z","timestamp":1788852835340,"version":"build-2803163510"},"reference-count":54,"publisher":"Oxford University Press (OUP)","license":[{"start":{"date-parts":[[2024,9,12]],"date-time":"2024-09-12T00:00:00Z","timestamp":1726099200000},"content-version":"vor","delay-in-days":255,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,1,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>In Australia, dystocia is responsible for 53% of lamb mortalities, and calcium deficiencies may be a contributing factor. A negative dietary cation\u2013anion difference (DCAD) diet can increase calcium concentrations in sheep. Therefore, this study aimed to investigate the effects of a negative DCAD diet on metabolic state, mineral status, and parturition duration in ewes compared with those fed a positive DCAD diet. At approximately day 130 of gestation (dG), 71 twin-bearing ewes were placed in the following treatment groups; ewes receiving a positive DCAD TMR (total mixed ration; DCAD of total diet\u2005=\u2005281.8 mEq\/kg DM; n\u2005=\u200535) and twin-bearing ewes receiving a negative DCAD TMR (DCAD of total diet\u2005=\u2005\u221289.0 mEq\/kg DM; n\u2005=\u200536). Urine and blood were sampled on dG 130, 140, and 145, and blood was also sampled at the onset of parturition and 4\u00a0h postpartum. Urine was analyzed for pH and blood was analyzed for metabolites, mineral concentration, and acid\u2013base balance. Lambs\u2019 liveweight, rectal temperature, blood glucose and lactate, and body morphology were measured. Serum phosphate concentrations at dG 145 were significantly lower for negative DCAD ewes compared with positive DCAD ewes (1.9\u2005\u00b1\u20050.1 vs. 2.1\u2005\u00b1\u20050.1 mmol\/L, P\u2005=\u20050.047). Ionized calcium (P\u2005=\u20050.09) and serum magnesium (P\u2005=\u20050.09) prepartum were marginally greater in the negative DCAD ewes (1.35\u2005\u00b1\u20050.06 and 1.06\u2005\u00b1\u20050.03 mmol\/L, respectively) compared with the positive DCAD ewes (1.18\u2005\u00b1\u20050.08 and 0.98\u2005\u00b1\u20050.04 mmol\/L, respectively). Urine pH was lower in the negative DCAD ewes compared with positive DCAD ewes at both dG 140 (7.38\u2005\u00b1\u20050.17 vs. and 8.10\u2005\u00b1\u20050.19. P\u2005=\u20050.01) and dG 145 (and 7.20\u2005\u00b1\u20050.19 vs. 8.25. P\u2005&amp;lt;\u20050.01). The birth interval between the first the second-born lamb was shorter in the negative DCAD ewes compared with the positive DCAD ewes (P\u2005=\u20050.02), but no differences in lamb survival or lamb viability (P\u2005&amp;gt;\u20050.05) were seen. The negative DCAD diet reduced parturition duration, most likely due to the marginally greater ionized calcium and magnesium concentrations. Despite this improvement, the negative DCAD ewes did not reach urinary acidification, indicating that the marginally significant greater ionized calcium and serum magnesium concentrations were due to the magnesium in the diets and not metabolic acidosis. Further research testing a negative DCAD diet that can achieve the target urine pH is required to determine whether this diet can decrease parturition duration and improve lamb viability.<\/jats:p>","DOI":"10.1093\/jas\/skae266","type":"journal-article","created":{"date-parts":[[2024,9,11]],"date-time":"2024-09-11T18:20:12Z","timestamp":1726078812000},"source":"Crossref","is-referenced-by-count":3,"title":["Effects of feeding a negative dietary cation and anion difference diet to twin-bearing Merino ewes in late gestation on parturition outcomes"],"prefix":"10.1093","volume":"102","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8215-6329","authenticated-orcid":false,"given":"Amy L","family":"Munn","sequence":"first","affiliation":[{"name":"Davies Livestock Research Centre, The University of Adelaide , Roseworthy, South Australia 5371 ,","place":["Australia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William H E J","family":"van Wettere","sequence":"additional","affiliation":[{"name":"Davies Livestock Research Centre, The University of Adelaide , Roseworthy, South Australia 5371 ,","place":["Australia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alyce M","family":"Swinbourne","sequence":"additional","affiliation":[{"name":"Turretfield Research Centre, South Australian Research and Development Institute , Rosedale, South Australia 5350 ,","place":["Australia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ian J","family":"Lean","sequence":"additional","affiliation":[{"name":"Scibus , Camden, New South Wales 2570 ,","place":["Australia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alice C","family":"Weaver","sequence":"additional","affiliation":[{"name":"Turretfield Research Centre, South Australian Research and Development Institute , Rosedale, South Australia 5350 ,","place":["Australia"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2024,9,12]]},"reference":[{"key":"2024100506311548600_CIT0001","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1016\/j.livsci.2018.10.001","article-title":"Effect of calcium and magnesium supplementation on minerals profile, immune responses, and energy profile of ewes and their lambs","volume":"217","author":"Ataollahi","year":"2018","journal-title":"Livest. 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