{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T14:12:19Z","timestamp":1783606339275,"version":"3.55.0"},"reference-count":49,"publisher":"Oxford University Press (OUP)","license":[{"start":{"date-parts":[[2024,1,1]],"date-time":"2024-01-01T00:00:00Z","timestamp":1704067200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,1,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>The mechanistic target of rapamycin (mTOR) predominantly regulates the expression and activity of placental nutrient transporters. The mTOR pathway can be activated by several nutrients, including the essential amino acid methionine. Additionally, previous research in nonruminant animals suggests that mTOR is influenced in a sexually dimorphic manner. In bovine, there is limited understanding of how maternal nutrition and offspring sexual dimorphism affect the placental transfer of nutrients. Thus, we investigated the effects of increasing the supply of dietary methionine to beef cattle heifers during mid-gestation on mTOR signaling, placental nutrient transporters, and fetal growth in male and female offspring. Forty purebred Angus heifers were used in a randomized complete block design experiment. From days 90 to 180 of gestation, heifers received a basal diet with no added methionine (CON, n\u2005=\u200520), or the basal diet plus 8.3\u00a0g of rumen-protected methionine (MET, n\u2005=\u200520) per animal daily. All animals received a basal diet in the first and third trimesters of gestation. Cotyledonary tissue samples were collected at parturition and utilized to examine the mTOR pathway and nutrient transporters through protein and gene expression analysis. The offspring\u2019s body weight was measured at birth. Data were analyzed using a mixed model that included the fixed effect of treatment, offspring sex, their interactions, and the random effect of block. At day 170 of gestation, MET-supplemented heifers showed higher plasma concentrations of methionine and glutamate (P\u2005&amp;lt;\u20050.01) and lower glycine and proline levels (P\u2005\u2264\u20050.01) compared to the CON group. A treatment\u2005\u00d7\u2005sex interaction was observed for calf birth weight (P\u2005=\u20050.03). In heifers that delivered male calves, MET supplementation increased the birth weight of the calves (P\u2005&amp;lt;\u20050.01). However, the dietary treatments had no effect on the birth weight of female calves (P\u2005=\u20050.32). The increase in birth weight of male calves from MET-fed heifers resembles the upregulation of placental mTOR and phosphorylated mTOR (P\u2005\u2264\u20050.03), as well as the amino acid transporters SLC1A5, SLC7A5, SLC38A6, and SLC38A11, and the glucose transporters SLC2A1 and SLC2A8 (P\u2005\u2264\u20050.05). Our findings suggest that increasing the supply of methionine to beef heifers during mid-gestation can modulate placental nutrient transport and fetal growth in a sex-dependent manner and that these effects are mediated, at least in part, by the mTOR pathway.<\/jats:p>","DOI":"10.1093\/jas\/skae305","type":"journal-article","created":{"date-parts":[[2024,10,11]],"date-time":"2024-10-11T07:03:56Z","timestamp":1728630236000},"source":"Crossref","is-referenced-by-count":7,"title":["Methionine supply during mid-gestation modulates the bovine placental mTOR pathway, nutrient transporters, and offspring birth weight in a sex-specific manner"],"prefix":"10.1093","volume":"102","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8317-4908","authenticated-orcid":false,"given":"Vin\u00edcius N","family":"Gouv\u00eaa","sequence":"first","affiliation":[{"name":"Texas A&M AgriLife Research and Extension Center , Amarillo, TX 79106 ,","place":["USA"]},{"name":"Department of Animal Science, Texas A&M University , Amarillo, TX 79106 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mackenzie M","family":"Smithyman","sequence":"additional","affiliation":[{"name":"Department of Animal and Range Science, Clayton Livestock Research Center, New Mexico State University , Clayton, NM 88415 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fernanda","family":"Hentz","sequence":"additional","affiliation":[{"name":"Department of Animal Sciences, University of Florida , Gainesville, FL 32611 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naghme","family":"Bagheri","sequence":"additional","affiliation":[{"name":"Department of Animal, Dairy, and Veterinary Sciences, Utah State University , Logan, UT 84322 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fernanda","family":"Batistel","sequence":"additional","affiliation":[{"name":"Department of Animal Sciences, University of Florida , Gainesville, FL 32611 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2024,10,11]]},"reference":[{"key":"2024110603361944100_CIT0001","volume-title":"Official methods of analysis","author":"AOAC","year":"2000","edition":"17th ed"},{"key":"2024110603361944100_CIT0002","doi-asserted-by":"crossref","first-page":"1047","DOI":"10.3168\/jds.2013-7348","article-title":"Isoleucine, leucine, methionine, and threonine effects on mammalian target of rapamycin signaling in mammary tissue","volume":"97","author":"Arriola Apelo","year":"2014","journal-title":"J. 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