{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T03:20:42Z","timestamp":1778815242554,"version":"3.51.4"},"reference-count":33,"publisher":"Oxford University Press (OUP)","license":[{"start":{"date-parts":[[2024,1,1]],"date-time":"2024-01-01T00:00:00Z","timestamp":1704067200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/100022822","name":"Merck Animal Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100022822","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,1,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>This study aimed to evaluate embryo yield in Holstein heifers superovulated with a single injection of recombinant, long-acting human follicle-stimulating hormone (rFSH) vs. multiple injections of pituitary-derived follicle-stimulating hormone (FSH). In experiment 1, heifers were assigned randomly to one of four experimental groups: Control (280\u00a0mg of pituitary-derived FSH; six injections of 40\u00a0mg and two injections of 20\u00a0mg, each ~12\u00a0h apart, n = 16); rFSH1 (50 \u00b5g of FSH analog protein, n =\u200516); rFSH2 (75 \u00b5g of FSH analog protein, n = 16); or rFSH3 (100 \u00b5g of FSH analog protein, n\u2005=\u200516). The rFSH was administered as a single injection. Estrous cycles were presynchronized with gonadorelin acetate (GnRH) and an intravaginal progesterone insert (CIDR) on day 8, followed by cloprostenol sodium (PGF2\u03b1) on day 3 and day 2 with removal of the CIDR, and GnRH on day 0. On day 5, ovarian follicles\u2005\u2265\u20055\u00a0mm in diameter were ablated by transvaginal ultrasound-guided aspiration. On day 6.5, the heifers received a CIDR and the first injection of either rFSH or pituitary-derived FSH. On day 9, the heifers received two injections of PGF2\u03b1, 12\u00a0h apart. On day 10.5, the CIDR was removed, and on day 11, the heifers received a GnRH injection. Heifers were AI-inseminated 12- and 24-h post-GnRH injection, and uterine contents were flushed transcervically on day 18 (7 d after the GnRH injection). Ovarian follicles\u2005\u2265\u20055\u00a0mm and corpora lutea (CL) were counted via ultrasound on days 5, 9, and 18. In experiment 1, group did not affect (P\u2005=\u20050.52) the number of follicles\u2005\u2265\u20055\u00a0mm (Control\u2005=\u200515.9\u2005\u00b1\u20051.2; rFSH1\u2005=\u200517.5\u2005\u00b1\u20051.3; rFSH2\u2005=\u200517.1\u2005\u00b1\u20051.3; rFHS 3\u2005=\u200518.6\u2005\u00b1\u20051.4 follicles) or the number of CL (P\u2005=\u20050.96) on day 9 (Control\u2005=\u20051.1\u2005\u00b1\u20050.3; rFSH1\u2005=\u20051.1\u2005\u00b1\u20050.3; rFSH2\u2005=\u20051.1\u2005\u00b1\u20050.3; rFSH3\u2005=\u20050.9\u2005\u00b1\u20050.2). Furthermore, there was no effect (P\u2005=\u20050.28) of rFSH dose on freezable embryos (grade 1 and 2 embryos) collected on day 18 (Control\u2005=\u20054.7\u2005\u00b1\u20051.1; rFSH1\u2005=\u20054.7\u2005\u00b1\u20051.2; rFSH2\u2005=\u20054.4\u2005\u00b1\u20051.1; rFSH3\u2005=\u20052.6\u2005\u00b1\u20050.7 embryos). In experiment 2, Control (n\u2005=\u20058) and rFSH1 (n\u2005=\u200516) groups were repeated in three replicates using the same protocols as experiment 1. Consequently, Results showed that rFSH produced fewer total number of ova\/embryos (Control\u2005=\u20059.9\u2005\u00b1\u20051.5 vs. rFHS1\u2005=\u20055.9\u2005\u00b1\u20050.9, P\u2005=\u20050.04) and fewer freezable embryos (Control\u2005=\u20055.3\u2005\u00b1\u20051.0 vs. rFSH1\u2005=\u20051.4\u2005\u00b1\u20050.3, P\u2005&amp;lt;\u20050.01). In conclusion, the single rFSH injection effectively induced superovulation; however, its repeated use reduced embryo production.<\/jats:p>","DOI":"10.1093\/jas\/skae326","type":"journal-article","created":{"date-parts":[[2024,10,24]],"date-time":"2024-10-24T18:49:41Z","timestamp":1729795781000},"source":"Crossref","is-referenced-by-count":1,"title":["Embryo production by Holstein heifers superovulated with a recombinant long-acting follicle-stimulating hormone analog"],"prefix":"10.1093","volume":"102","author":[{"given":"Ahmadreza","family":"Mirzaei","sequence":"first","affiliation":[{"name":"Department of Large Animal Clinical Sciences, University of Florida , Gainesville, FL 32610 ,","place":["USA"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2884-6035","authenticated-orcid":false,"given":"Maria C","family":"Londo\u00f1o-Mendez","sequence":"additional","affiliation":[{"name":"North Florida Research and Education Center, Department of Animal Sciences, University of Florida , Marianna, FL 32443 ,","place":["USA"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2161-4648","authenticated-orcid":false,"given":"Sergio","family":"Lasso-Ramirez","sequence":"additional","affiliation":[{"name":"North Florida Research and Education Center, Department of Animal Sciences, University of Florida , Marianna, FL 32443 ,","place":["USA"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paul E","family":"Adams","sequence":"additional","affiliation":[{"name":"R & D Pharmaceuticals, Merck Animal Health , Rahway, NJ 07065 ,","place":["USA"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zachary K","family":"Seekford","sequence":"additional","affiliation":[{"name":"Department of Animal Sciences, University of Florida , Gainesville, FL 32610 ,","place":["USA"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5438-2137","authenticated-orcid":false,"given":"John J","family":"Bromfield","sequence":"additional","affiliation":[{"name":"Department of Animal Sciences, University of Florida , Gainesville, FL 32610 ,","place":["USA"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1315-743X","authenticated-orcid":false,"given":"Daniella","family":"Heredia","sequence":"additional","affiliation":[{"name":"North Florida Research and Education Center, Department of Animal Sciences, University of Florida , Marianna, FL 32443 ,","place":["USA"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5174-3427","authenticated-orcid":false,"given":"Nicolas","family":"Di Lorenzo","sequence":"additional","affiliation":[{"name":"North Florida Research and Education Center, Department of Animal Sciences, University of Florida , Marianna, FL 32443 ,","place":["USA"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ricardo C","family":"Chebel","sequence":"additional","affiliation":[{"name":"Department of Large Animal Clinical Sciences, University of Florida , Gainesville, FL 32610 ,","place":["USA"]},{"name":"Department of Animal Sciences, University of Florida , Gainesville, FL 32610 ,","place":["USA"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7446-3831","authenticated-orcid":false,"given":"Angela M","family":"Gonella-Diaza","sequence":"additional","affiliation":[{"name":"North Florida Research and Education Center, Department of Animal Sciences, University of Florida , Marianna, FL 32443 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