{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T19:09:11Z","timestamp":1760123351459,"version":"3.41.2"},"reference-count":37,"publisher":"Oxford University Press (OUP)","license":[{"start":{"date-parts":[[2024,12,4]],"date-time":"2024-12-04T00:00:00Z","timestamp":1733270400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100008119","name":"Norwegian University of Life Sciences","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100008119","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Felleskj\u00f8pet F\u00f4rutvikling"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,1,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Feeding order of forage and concentrate might affect gastric emptying and subsequently digestion in horses. The objective of this study was to measure gastric emptying in combination with metabolic and digestive responses in the plasma and cecum, respectively, when changing the feeding order of oats (O) and hay (H) (oats first, then hay: O\u2013H vs. hay first, then oats: H\u2013O). Four cecum cannulated horses were used in a 2\u2005\u00d7\u20052 crossover design, with two 12-d periods consisting of 10 d of diet adaptation and 2 d of data collection. Hay was fed at 0600, 1400, and 2000 hours, while oats were fed in the morning either 15\u00a0min before or 1\u00a0h after feeding hay. On days of data collection, baseline samples were collected before feeding 1.4\u00a0kg dry matter (DM) hay and 474\u00a0g DM oats (0.4\u00a0g starch\/kg body weight), and data were collected until 8\u00a0h after feeding. Gastric emptying of oats was estimated using the 13C acetic acid breath test, where breath samples were analyzed for a 12C:13C ratio after administration of 13C acetic acid mixed with oats. Gastric emptying coefficient (GEC), time where half of the total cumulative recovery of 13C was excreted (t1\/2), and time where the maximal amount of 13C was excreted (Tmax) were calculated. Samples of blood and cecal fluid were collected at hourly intervals. Blood plasma was analyzed for glucose and insulin, and baseline concentrations, peak concentrations, time of peaks, and area under the curves were calculated. A pH probe was placed in the cecum measuring pH every minute to find minimum pH and time to reach minimum pH. Hourly cecal samples were analyzed for pH and short-chained fatty acid (SCFA) concentrations. Results from the 13C acetic acid breath test indicated that feeding order affected gastric emptying, as Tmax was longer (P\u2005=\u20050.004) when feeding H\u2013O (2.18\u00a0h) than O\u2013H (1.09\u00a0h), but there was no effect on the GEC and T1\/2. No effect of feeding order was found for plasma glucose and insulin measures. Feeding order had no effect on minimum pH, but the time to reach minimum pH increased (P\u2005=\u20050.014) from 170\u00a0min for O\u2013H to 280\u00a0min for H\u2013O, and average pH was lower in the intervals 0\u2013170\u00a0min (P\u2005=\u20050.006) and 170\u2013280\u00a0min (P\u2005=\u20050.006) for O\u2013H than H\u2013O. In general, the time of sampling had a larger effect on SCFA concentrations than feeding order. In conclusion, this study indicates that feeding order affected gastric emptying, and the digestive and metabolic responses were more clearly reflected in cecum pH than in plasma glucose and insulin.<\/jats:p>","DOI":"10.1093\/jas\/skae368","type":"journal-article","created":{"date-parts":[[2024,12,10]],"date-time":"2024-12-10T18:28:53Z","timestamp":1733855333000},"source":"Crossref","is-referenced-by-count":1,"title":["The effect of feeding order of forage and oats on metabolic and digestive responses related to gastric emptying in horses"],"prefix":"10.1093","volume":"103","author":[{"given":"Rasmus Bovbjerg","family":"Jensen","sequence":"first","affiliation":[{"name":"Department of Animal and Aquacultural Sciences, Norwegian University of Life Sciences , \u00c5s,","place":["Norway"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ingrid Hornnes","family":"Walslag","sequence":"additional","affiliation":[{"name":"Department of Paraclinical Sciences, Norwegian University of Life Sciences , \u00c5s,","place":["Norway"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Caroline","family":"Marcussen","sequence":"additional","affiliation":[{"name":"Department of Veterinary Clinical Sciences, University of Copenhagen , Frederiksberg ,","place":["Denmark"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nana Wentzel","family":"Thorringer","sequence":"additional","affiliation":[{"name":"Department of Animal and Aquacultural Sciences, Norwegian University of Life Sciences , \u00c5s,","place":["Norway"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter","family":"Junghans","sequence":"additional","affiliation":[{"name":"Institute of Nutritional Physiology \u2018Oskar Kellner\u2019, Research Institute for Farm Animal Biology (FBN) , Dummerstorf ,","place":["Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicole Frost","family":"Nyquist","sequence":"additional","affiliation":[{"name":"Department of Paraclinical Sciences, Norwegian University of Life Sciences , \u00c5s,","place":["Norway"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2024,12,4]]},"reference":[{"journal-title":"Neutral detergent fiber in feeds \u2013 filter bag technique (for A200 and A200I)","year":"2017","author":"ANKOM","key":"2025012109554923900_CIT0035"},{"journal-title":"Acid detergent fiber in feeds \u2013 filter bag technique (for A300 and A200I)","year":"2017","author":"ANKOM","key":"2025012109554923900_CIT0034"},{"key":"2025012109554923900_CIT0001","doi-asserted-by":"publisher","first-page":"1048","DOI":"10.1016\/0016-5085(95)90202-3","article-title":"The [13C]acetate breath test accurately reflects gastric emptying of liquids in both liquid and semisolid test meals","volume":"108","author":"Braden","year":"1995","journal-title":"Gastroenterology"},{"key":"2025012109554923900_CIT0002","doi-asserted-by":"publisher","first-page":"18621","DOI":"10.1038\/s41598-019-54039-8","article-title":"High-starch diets alter equine faecal microbiota and increase behavioural reactivity","volume":"9","author":"Bulmer","year":"2019","journal-title":"Sci. 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