{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T23:24:27Z","timestamp":1787009067997,"version":"build-2736575974"},"reference-count":41,"publisher":"Oxford University Press (OUP)","funder":[{"DOI":"10.13039\/100013183","name":"Swine Innovation Porc","doi-asserted-by":"publisher","award":["1795"],"award-info":[{"award-number":["1795"]}],"id":[{"id":"10.13039\/100013183","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100020072","name":"Lallemand","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100020072","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Centre de Recherche en Sciences Animales de Deschambault"},{"name":"Agri-March\u00e9"},{"DOI":"10.13039\/501100000040","name":"Agriculture and Agri-Food Canada","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000040","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,1,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>This study compared different levels of dietary zinc oxide (ZnO) on mitochondria ATP synthesis and the cellular and systemic redox balance in weaned pigs. One hundred twenty weaned piglets (7.96\u2005\u00b1\u20051.17\u00a0kg, 21 days of age) were randomly allocated in one of three diets containing different Zn levels (as ZnO); 100 (100Zn), 1,000 (1000Zn) and 3,000\u00a0mg\/kg (3000Zn), and were slaughtered at day 21, 23, 35 or 42 for the collection of blood and liver samples. Dietary copper (Cu) levels were constant at 131\u00a0mg\/kg. Hepatic mitochondrial concentrations of Zn and Cu, hepatic mitochondria respiration, antioxidant response, and the hepatic expression of related genes were analyzed. Piglets fed 3000Zn had the highest Zn (P\u2005&amp;lt;\u20050.01) and the lowest Cu concentrations (P\u2005=\u20050.01) in hepatic mitochondria. The hepatic oxygen consumption rate for maximal respiration was the highest in 3000Zn piglets (P\u2005&amp;lt;\u20050.05), whereas hepatic cytochrome c oxidase activity tended to be lower (P\u2005=\u20050.06) and intracellular ATP concentrations were the lowest (P\u2005=\u20050.01). Mitochondrial Cu\/Zn superoxide dismutase (Cu\/Zn-SOD) activity was the lowest (P\u2005&amp;lt;\u20050.01) and glutathione peroxidase (GPx) activity tended to be the lowest (P\u2005=\u20050.07) for 3000Zn piglets. The mRNA expression of the antioxidant-related genes copper chaperone for superoxide dismutase (CCS) was highest (P\u2005=\u20050.01) in 3000Zn piglets and tended to be the highest (P\u2005=\u20050.07) for glutathione synthetase (GSS). Plasma carbonyls concentrations (P\u2005=\u20050.02) and GPx activity (P\u2005=\u20050.01) were the highest while SOD activity was not impacted (P\u2005=\u20050.55) in 3000Zn piglets. In conclusion, supplementing 3000\u00a0mg Zn\/kg had detrimental impacts on mitochondria Zn and Cu homeostasis, resulting in a dysfunctional mitochondria respiratory chain and disturbed antioxidant response in postweaning piglets.<\/jats:p>","DOI":"10.1093\/jas\/skaf031","type":"journal-article","created":{"date-parts":[[2025,2,6]],"date-time":"2025-02-06T02:49:08Z","timestamp":1738810148000},"source":"Crossref","is-referenced-by-count":5,"title":["Effects of increasing dietary zinc oxide levels on the hepatic mitochondrial energy metabolism, oxidative balance, and antioxidant system in weaned piglets"],"prefix":"10.1093","volume":"103","author":[{"given":"Genevi\u00e8ve","family":"Villeneuve","sequence":"first","affiliation":[{"name":"Sherbrooke Research and Development Centre, Agriculture and Agri-Food Canada , Sherbrooke, QC,","place":["Canada"]},{"name":"Biology Department, Universit\u00e9 de Sherbrooke , Sherbrooke, QC,","place":["Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caroline","family":"Roy","sequence":"additional","affiliation":[{"name":"Sherbrooke Research and Development Centre, Agriculture and Agri-Food Canada , Sherbrooke, QC,","place":["Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karine","family":"Desch\u00eane","sequence":"additional","affiliation":[{"name":"Sherbrooke Research and Development Centre, Agriculture and Agri-Food Canada , Sherbrooke, QC,","place":["Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0134-6148","authenticated-orcid":false,"given":"Jean-Jacques","family":"Matte","sequence":"additional","affiliation":[{"name":"Sherbrooke Research and Development Centre, Agriculture and Agri-Food Canada , Sherbrooke, QC,","place":["Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00e9r\u00f4me","family":"Lapointe","sequence":"additional","affiliation":[{"name":"Sherbrooke Research and Development Centre, Agriculture and Agri-Food Canada , Sherbrooke, QC,","place":["Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Danyel Bueno","family":"Dalto","sequence":"additional","affiliation":[{"name":"Sherbrooke Research and Development Centre, Agriculture and Agri-Food Canada , Sherbrooke, QC,","place":["Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2025,2,6]]},"reference":[{"key":"2025032307515953300_CIT0001","doi-asserted-by":"publisher","first-page":"5245","DOI":"10.1158\/0008-5472.CAN-04-0496","article-title":"Normalization of real-time quantitative reverse transcription-PCR data: a model-based variance estimation approach to identify genes suited for normalization, applied to bladder and colon cancer data sets","volume":"64","author":"Andersen","year":"2004","journal-title":"Cancer Res"},{"key":"2025032307515953300_CIT0002","doi-asserted-by":"publisher","first-page":"1501","DOI":"10.1039\/c7mt00221a","article-title":"The mitochondrion: a central architect of copper homeostasis","volume":"9","author":"Baker","year":"2017","journal-title":"Metallomics"},{"key":"2025032307515953300_CIT0005","doi-asserted-by":"publisher","first-page":"316","DOI":"10.1016\/j.jnutbio.2004.02.004","article-title":"Maintaining copper homeostasis: regulation of copper-trafficking proteins in response to copper deficiency or overload","volume":"15","author":"Bertinato","year":"2004","journal-title":"J. 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