{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T11:52:00Z","timestamp":1791546720592,"version":"4.3.4"},"reference-count":34,"publisher":"Oxford University Press (OUP)","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,1,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>The ergot alkaloid ergovaline is a potent vasoconstrictor. Previous research has shown that ergovaline can bind serotonin (5-HT) receptors and elicit vasoconstrictive activity. Three experiments were conducted to evaluate 5-HT as a vasorelaxant in the ovine saphenous artery and vein. Blood vessels were collected from mixed-breed market ram lambs (n\u2005=\u200523) at slaughter. Experiment 1: artery and vein cross-sections were precontracted with 1\u2005\u00d7\u200510\u22124 M phenylephrine in a multimyograph for 15\u00a0min, exposed to concentrations of 5-HT that ranged from 1\u2005\u00d7\u200510\u22129 to 1\u2005\u00d7\u200510\u22124 M for a 5-min interval. Response data were normalized to the 1\u2005\u00d7\u200510\u22124 M phenylephrine response and were analyzed as a completely randomized design in SAS. The lateral saphenous vein (n\u2005=\u20055 lambs) relaxed to increasing concentrations of 5-HT (P\u2005&amp;lt;\u20050.05) with 65% to 75 % relaxation occurring at 1\u2005\u00d7\u200510\u22127 M through 1\u2005\u00d7\u200510\u22124 5-HT. Conversely, the lateral saphenous artery (n\u2005=\u20056 lambs) contracted in response to increasing concentrations of 5-HT (P\u2005&amp;lt;\u20050.05) resulting in a 255% increase from the phenylephrine response by the 1\u2005\u00d7\u200510\u22124 M addition. Experiment 2: saphenous veins from lambs (n\u2005=\u20055) were exposed to increasing concentrations of selective 5-HT receptor agonists to determine the receptor subtypes involved in the previously observed vasorelaxation. Agonists for receptors 5-HT2B, 5-HT4, and 5-HT7 were used. While all three 5-HT receptor subtypes resulted in vasorelaxation (P\u2005&amp;lt;\u20050.05), the 5-HT2B agonist only stimulated a maximal relaxation of 32% compared to the agonists for 5-HT4 and 5-HT7 that resulted in maximal relaxation of 68% and 50%, respectively. Experiment 3: saphenous arteries and veins were collected from market rams (n\u2005=\u20057) and precontracted with 1\u2005\u00d7\u200510\u22126 M ergovaline for 30\u00a0min. The lateral saphenous vein relaxed to increasing concentrations of 5-HT (P\u2005&amp;lt;\u20050.05) with 87 % relaxation occurring by 1\u2005\u00d7\u200510\u22126 M 5-HT. Conversely, the lateral saphenous artery contracted in response to increasing concentrations of 5-HT (P\u2005&amp;lt;\u20050.05) resulting in a 43% increase from the ergovaline response by the 1\u2005\u00d7\u200510\u22124 M addition. This is the first reported direct observation of stimulated relaxation of a blood vessel constricted by ergovaline. The agonist for 5-HT4 produced the greatest relaxation in the ovine lateral saphenous vein. Serotonin receptors involved in 5-HT-mediated vasorelaxation do not appear to be antagonized by ergovaline and should be targeted in subsequent research focused on mitigation of ergot alkaloid-induced vasoconstriction.<\/jats:p>","DOI":"10.1093\/jas\/skaf108","type":"journal-article","created":{"date-parts":[[2025,4,15]],"date-time":"2025-04-15T09:20:55Z","timestamp":1744708855000},"source":"Crossref","is-referenced-by-count":3,"title":["Serotonin can stimulate vasorelaxation in ovine lateral saphenous veins precontracted with ergovaline"],"prefix":"10.1093","volume":"103","author":[{"given":"James L","family":"Klotz","sequence":"first","affiliation":[{"name":"USDA-ARS, Forage-Animal Production Research Unit , Lexington, KY 40546-0091 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Celina M","family":"Checura","sequence":"additional","affiliation":[{"name":"Piedmont Research and Education Center, Clemson University , Clemson, SC 29634 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maslyn A","family":"Greene","sequence":"additional","affiliation":[{"name":"Department of Animal and Veterinary Science, Clemson University , Clemson, SC 29634-0311 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7528-1036","authenticated-orcid":false,"given":"Huihua","family":"Ji","sequence":"additional","affiliation":[{"name":"Kentucky Tobacco Research and Development Center, University of Kentucky , Lexington, KY 40546 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4057-0062","authenticated-orcid":false,"given":"Susan K","family":"Duckett","sequence":"additional","affiliation":[{"name":"Department of Animal and Veterinary Science, Clemson University , Clemson, SC 29634-0311 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2025,4,15]]},"reference":[{"key":"2025052714112016700_CIT0001","volume-title":"Ergot compounds: a synopsis","author":"Berde","year":"1980"},{"key":"2025052714112016700_CIT0002","doi-asserted-by":"publisher","first-page":"355","DOI":"10.1146\/annurev.med.60.042307.110802","article-title":"The expanded biology of serotonin","volume":"60","author":"Berger","year":"2009","journal-title":"Annu. 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