{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T04:02:45Z","timestamp":1791950565802,"version":"4.3.4"},"reference-count":34,"publisher":"Oxford University Press (OUP)","license":[{"start":{"date-parts":[[2025,9,8]],"date-time":"2025-09-08T00:00:00Z","timestamp":1757289600000},"content-version":"vor","delay-in-days":250,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100013699","name":"Austrian Federal Ministry of Education, Science and Research","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100013699","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Austrian Federal Ministry for Digital and Economic Affairs"},{"DOI":"10.13039\/100010132","name":"National Foundation for Research, Technology and Development","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100010132","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100009973","name":"BIOMIN","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100009973","id-type":"DOI","asserted-by":"publisher"}]},{"name":"GmbH"},{"name":"Christian Doppler Laboratory for Innovative Gut Health Concepts of Livestock"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,1,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>It is helpful for diagnostic purposes to improve our current knowledge of gut development and serum biochemistry in young piglets. This study investigated serum biochemistry, gut site-specific patterns of short-chain fatty acids (SCFA), and expression of genes related to barrier function, innate immune response, antioxidative status, and sensing of fatty and bile acids in suckling and newly weaned piglets. The experiment consisted of two replicate batches with 10 litters each. Piglets could suckle freely and had access to creep feed from day of life (DoL) 3. Weaning occurred on DoL28. Blood, gastric, cecal, and colonic digesta, as well as jejunal and cecal tissue were collected on DoL3, 7, 14, 21, 28, 31, and 35 (n\u2009=\u200910\/sex\/DoL). Serum liver enzyme activities were high on DoL3 but decreased thereafter, reflecting the immature state of hepatocytes after birth (P\u2009&amp;lt;\u20090.05). Age-related fluctuations in serum glucose and lipids indicated changes in energy metabolism within the suckling period and lower feed intake after weaning. Short-chain fatty acids increased in cecal digesta from DoL3 to 28 (P\u2009&amp;lt;\u20090.05). After weaning, lower gastric and cecal SCFA on DoL31 and DoL35, respectively, versus DoL28 mirrored lower feed intake, whereas colonic SCFA increased after weaning (P\u2009&amp;lt;\u20090.05). Jejunal and cecal expression of free fatty acid receptors and monocarboxylate transporters changed with increasing age (P\u2009&amp;lt;\u20090.05). For some genes including FFAR2 and FFAR3 in the cecum, expression levels declined from DoL3 onwards (P\u2009&amp;lt;\u20090.05), indicating an inverse relationship with luminal SCFA availability. However, there was no unique jejunal and cecal expression pattern for fatty acid receptors and transporters, and pattern recognition receptors (PRR), probably corresponding to the age-related changes in their ligands. Increasing expression of anti-inflammatory IL10 in jejunum and cecum from DoL3 to 28 may be indicative of a build-up of immune tolerance (P\u2009&amp;lt;\u20090.05). Postweaning expression of PRR was not increased, but reduced jejunal expression of antioxidative enzymes and increased cecal expression of proinflammatory TNFA combined with lower expression of MUC2, OCLN, and ZO1 compared to the suckling phase indicated compromised gut homeostasis (P\u2009&amp;lt;\u20090.05). Overall, the present results show study-specific age-related patterns of genes associated with mucosal metabolite sensing or defense mechanisms in the jejunum and cecum from birth to after weaning.<\/jats:p>","DOI":"10.1093\/jas\/skaf313","type":"journal-article","created":{"date-parts":[[2025,9,8]],"date-time":"2025-09-08T15:24:03Z","timestamp":1757345043000},"source":"Crossref","is-referenced-by-count":2,"title":["Age-related evolution of serum biochemistry and intestinal fatty acid signaling, innate immune response, and barrier function in suckling and newly weaned piglets"],"prefix":"10.1093","volume":"103","author":[{"given":"Fitra","family":"Yosi","sequence":"first","affiliation":[{"name":"Centre for Veterinary Systems Transformation and Sustainability, Clinical Department for Farm Animals and Food System Science, University of Veterinary Medicine Vienna , Vienna 1210,","place":["Austria"]},{"name":"Christian-Doppler Laboratory for Innovative Gut Health Concepts of Livestock, Institute of Animal Nutrition and Functional Plant Compounds, Centre for Animal Nutrition and Welfare, University of Veterinary Medicine Vienna , Vienna 1210,","place":["Austria"]},{"name":"Department of Animal Science, Faculty of Agriculture, University of Sriwijaya , Palembang 30662,","place":["Indonesia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kristina","family":"Hartinger","sequence":"additional","affiliation":[{"name":"Centre for Veterinary Systems Transformation and Sustainability, Clinical Department for Farm Animals and Food System Science, University of Veterinary Medicine Vienna , Vienna 1210,","place":["Austria"]},{"name":"Christian-Doppler Laboratory for Innovative Gut Health Concepts of Livestock, Institute of Animal Nutrition and Functional Plant Compounds, Centre for Animal Nutrition and Welfare, University of Veterinary Medicine Vienna , Vienna 1210,","place":["Austria"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frederike","family":"Lerch","sequence":"additional","affiliation":[{"name":"Centre for Veterinary Systems Transformation and Sustainability, Clinical Department for Farm Animals and Food System Science, University of Veterinary Medicine Vienna , Vienna 1210,","place":["Austria"]},{"name":"Christian-Doppler Laboratory for Innovative Gut Health Concepts of Livestock, Institute of Animal Nutrition and Functional Plant Compounds, Centre for Animal Nutrition and Welfare, University of Veterinary Medicine Vienna , Vienna 1210,","place":["Austria"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julia C","family":"V\u00f6tterl","sequence":"additional","affiliation":[{"name":"Centre for Veterinary Systems Transformation and Sustainability, Clinical Department for Farm Animals and Food System Science, University of Veterinary Medicine Vienna , Vienna 1210,","place":["Austria"]},{"name":"Christian-Doppler Laboratory for Innovative Gut Health Concepts of Livestock, Institute of Animal Nutrition and Functional Plant Compounds, Centre for Animal Nutrition and Welfare, University of Veterinary Medicine Vienna , Vienna 1210,","place":["Austria"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simone","family":"Koger","sequence":"additional","affiliation":[{"name":"Christian-Doppler Laboratory for Innovative Gut Health Concepts of Livestock, Institute of Animal Nutrition and Functional Plant Compounds, Centre for Animal Nutrition and Welfare, University of Veterinary Medicine Vienna , Vienna 1210,","place":["Austria"]},{"name":"Centre for Animal Nutrition and Welfare, Clinical Department for Farm Animals and Food System Science, University of Veterinary Medicine Vienna , Vienna 1210,","place":["Austria"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suchitra","family":"Sharma","sequence":"additional","affiliation":[{"name":"Christian-Doppler Laboratory for Innovative Gut Health Concepts of Livestock, Institute of Animal Nutrition and Functional Plant Compounds, Centre for Animal Nutrition and Welfare, University of Veterinary Medicine Vienna , Vienna 1210,","place":["Austria"]},{"name":"Centre for Animal Nutrition and Welfare, Clinical Department for Farm Animals and Food System Science, University of Veterinary Medicine Vienna , Vienna 1210,","place":["Austria"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Doris","family":"Verhovsek","sequence":"additional","affiliation":[{"name":"Clinical Centre for Population Medicine in Fish, Pig and Poultry, Clinical Department for Farm Animals and Food System Science, University of Veterinary Medicine Vienna , Vienna 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