{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,26]],"date-time":"2025-11-26T03:45:42Z","timestamp":1764128742818,"version":"3.46.0"},"reference-count":41,"publisher":"Oxford University Press (OUP)","license":[{"start":{"date-parts":[[2025,11,6]],"date-time":"2025-11-06T00:00:00Z","timestamp":1762387200000},"content-version":"vor","delay-in-days":309,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,1,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>This study compared the short-term effects of the laparoscopic and semi-laparoscopic embryo transfer (ET) procedures on salivary cortisol concentrations and the lying behavior of ewes. In total, 40 ewes were synchronized for ET and placed randomly into individual pens 2\u2009d before the operations. On the day of the operations, laparoscopic (L, n\u2009=\u200915) and semi-laparoscopic (SL, n\u2009=\u200910) ET were performed. At the same time, control animals (Control, n\u2009=\u200915) were placed into the laparoscopic cradle for 6\u2009min without an ET procedure. Monitoring of standing and lying behaviors started 24\u2009h before the surgery and lasted until 24\u2009h after the procedures were completed. Saliva samples were taken 4 times during the experiment and assayed for cortisol concentrations. Saliva cortisol concentrations were elevated in all groups 1 (Control: 4.8\u2009\u00b1\u20090.5\u2009ng\/mL; L: 5.1\u2009\u00b1\u20090.5\u2009ng\/mL; SL: 4.2\u2009\u00b1\u20090.7\u2009ng\/mL) and 2 (Control: 5.5\u2009\u00b1\u20090.5\u2009ng\/mL; L: 5.9\u2009\u00b1\u20090.5\u2009ng\/mL; SL: 4.8\u2009\u00b1\u20090.7\u2009mg\/mL) hours after the procedures compared to the initial concentration (Control: 3.8\u2009\u00b1\u20090.5\u2009ng\/mL; L: 3.7\u2009\u00b1\u20090.5\u2009ng\/mL; SL: 3.1\u2009\u00b1\u20090.7\u2009ng\/mL), and returned to the basal levels for the end of the 24-h post-surgery period (Control: 3.4\u2009\u00b1\u20090.5\u2009ng\/mL; L: 4.1\u2009\u00b1\u20090.5\u2009ng\/mL; SL: 3.5\u2009\u00b1\u20090.7\u2009ng\/mL). There were no significant differences in the cortisol levels among the groups. There was no difference in the total lying time between the groups 24\u2009h before or after the surgery. The average length of lying bouts calculated for the 24-h post-surgery period increased in all groups from the 24-h pre-surgery period, with a parallel decrement in the number of lying bouts (P\u2009&amp;lt;\u20090.05). Our results suggest that the laparoscopic and semi-laparoscopic ET did not cause more stress in the ewes than the handling procedures related to surgery and preparation.<\/jats:p>","DOI":"10.1093\/jas\/skaf381","type":"journal-article","created":{"date-parts":[[2025,11,9]],"date-time":"2025-11-09T06:56:49Z","timestamp":1762671409000},"source":"Crossref","is-referenced-by-count":0,"title":["Short communication: salivary cortisol concentrations and lying behavior of ewes in response to semi-laparoscopic and laparoscopic embryo transfer"],"prefix":"10.1093","volume":"103","author":[{"given":"Melinda","family":"Bagi","sequence":"first","affiliation":[{"name":"Department of Animal Husbandry, Faculty of Agricultural and Food Sciences and Environmental Management, Institute of Animal Science, Biotechnology and Nature Conservation, University of Debrecen , Debrecen,","place":["Hungary"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Viktor","family":"Jurkovich","sequence":"additional","affiliation":[{"name":"Centre for Animal Welfare, University of Veterinary Medicine , Budapest,","place":["Hungary"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Levente","family":"Kov\u00e1cs","sequence":"additional","affiliation":[{"name":"Department of Animal Husbandry and Animal Welfare, Institute of Animal Sciences, Hungarian University of Agriculture and Life Sciences Institute of Animal Science , G\u00f6d\u00f6ll\u0151,","place":["Hungary"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Szil\u00e1rd","family":"Bod\u00f3","sequence":"additional","affiliation":[{"name":"Department of Precision Livestock Farming and Animal Biotechnics, Hungarian University of Agriculture and Life Sciences, Institute of Animal Science , Kaposv\u00e1r,","place":["Hungary"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mikolt","family":"Bakony","sequence":"additional","affiliation":[{"name":"Centre for Translational Medicine, Semmelweis University , Budapest,","place":["Hungary"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J\u00e1nos","family":"Ol\u00e1h","sequence":"additional","affiliation":[{"name":"Department of Animal Husbandry, Faculty of Agricultural and Food Sciences and Environmental Management, Institute of Animal Science, Biotechnology and Nature Conservation, University of Debrecen , Debrecen,","place":["Hungary"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L\u00e1szl\u00f3","family":"Huzsvai","sequence":"additional","affiliation":[{"name":"Department of Quantitative Methodology, Faculty of Economics and Business, Institute for Methodology and Business Digitalisation, University of Debrecen , Debrecen,","place":["Hungary"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N\u00f3ra","family":"Vass","sequence":"additional","affiliation":[{"name":"Department of Animal Husbandry, Faculty of Agricultural and Food Sciences and Environmental Management, Institute of Animal Science, Biotechnology and Nature Conservation, University of Debrecen , Debrecen,","place":["Hungary"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2025,11,6]]},"reference":[{"issue":"3-4","key":"2025112522415167100_skaf381-B1","doi-asserted-by":"publisher","first-page":"152","DOI":"10.1016\/j.anireprosci.2012.01.009","article-title":"Assisted reproductive technologies in the reproductive management of small ruminants","volume":"130","author":"Amiridis","year":"2012","journal-title":"Anim. 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