{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T09:28:05Z","timestamp":1791538085081,"version":"4.3.4"},"reference-count":70,"publisher":"Oxford University Press (OUP)","license":[{"start":{"date-parts":[[2025,11,29]],"date-time":"2025-11-29T00:00:00Z","timestamp":1764374400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,1,8]]},"abstract":"<jats:title>Lay Summary<\/jats:title>\n                  <jats:p>To meet the growing demand for beef driven by a rising population, cattle operations must improve efficiency of nutrient utilization by the rumen and the entire digestive tract. This can be achieved by enhancing forage quality or implementing nutritional strategies that optimize forage and overall nutrient digestibility, such as supplementing cattle with Bacillus-based probiotics. Reproductive efficiency is a critical determinant of cattle availability for beef production, and probiotics may enhance fertility by modulating the microbial communities in the female reproductive tract. Therefore, this experiment evaluated the effects of a Bacillus-based probiotic on nutrient utilization, physiological responses, and reproductive tract microbiota of beef heifers fed a forage-based diet. Results showed improved ruminal and overall nutrient utilization with probiotic supplementation. Changes in the reproductive bacterial communities were limited to vagina, but suggested a healthier vaginal environment. Collectively, this experiment provides novel insights on how supplementing a Bacillus-based probiotic can enhance the utilization of forage nutrients by growing beef cattle, with benefits to vaginal microbiota that may result in improved fertility.<\/jats:p>","DOI":"10.1093\/jas\/skaf411","type":"journal-article","created":{"date-parts":[[2025,11,26]],"date-time":"2025-11-26T13:07:28Z","timestamp":1764162448000},"source":"Crossref","is-referenced-by-count":8,"title":["Effects of feeding a\n                    <i>Bacillus<\/i>\n                    -based probiotic on nutrient utilization and microbiota profile in the rumen and reproductive tract of beef heifers receiving a forage-based diet"],"prefix":"10.1093","volume":"104","author":[{"given":"Izadora S","family":"De Souza","sequence":"first","affiliation":[{"name":"Department of Animal Science, Texas A&M University , College Station, TX 77843"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7497-3601","authenticated-orcid":false,"given":"Reinaldo F","family":"Cooke","sequence":"additional","affiliation":[{"name":"Department of Animal Science, Texas A&M University , College Station, TX 77843"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shea J","family":"Mackey","sequence":"additional","affiliation":[{"name":"Department of Animal Science, Texas A&M University , College Station, TX 77843"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thain\u00e1","family":"Minela","sequence":"additional","affiliation":[{"name":"Department of Animal Science, Texas A&M University , College Station, TX 77843"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zachary K","family":"Seekford","sequence":"additional","affiliation":[{"name":"Department of Animal Science, Texas A&M University , College Station, TX 77843"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giuseppe","family":"Copani","sequence":"additional","affiliation":[{"name":"Novonesis , Lyngby 2800,","place":["Denmark"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raphaele","family":"Gresse","sequence":"additional","affiliation":[{"name":"Novonesis , Lyngby 2800,","place":["Denmark"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kelsey M","family":"Harvey","sequence":"additional","affiliation":[{"name":"Prairie Research Unit, Mississippi State University , Prairie, MS 39756"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ky G","family":"Pohler","sequence":"additional","affiliation":[{"name":"Department of Animal Science, Texas A&M University , College Station, TX 77843"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3383-1620","authenticated-orcid":false,"given":"Jo\u00e3o M B","family":"Vendramini","sequence":"additional","affiliation":[{"name":"Stephenville Research and Extension Center, Texas A&M AgriLife Research , Stephenville, TX 76401"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1819-4013","authenticated-orcid":false,"given":"Bruno I","family":"Cappellozza","sequence":"additional","affiliation":[{"name":"Novonesis , Lyngby 2800,","place":["Denmark"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2025,11,29]]},"reference":[{"issue":"7","key":"2026011206201542600_skaf411-B1","doi-asserted-by":"publisher","first-page":"1598","DOI":"10.3168\/jds.S0022-0302(00)75030-2","article-title":"Effects of diet on short-term regulation of feed intake by lactating dairy cattle","volume":"83","author":"Allen","year":"2000","journal-title":"J. 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