{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T06:06:51Z","timestamp":1788847611742,"version":"build-2803163510"},"reference-count":47,"publisher":"Oxford University Press (OUP)","license":[{"start":{"date-parts":[[2026,1,7]],"date-time":"2026-01-07T00:00:00Z","timestamp":1767744000000},"content-version":"vor","delay-in-days":6,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,1,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>No study is currently available regarding the roles of the amino acid transporters in promoting mineral absorption in animals. To study whether the y(+)L-type amino acid transporter 2 (y+LAT2) participates in enhancing Zn absorption as Zn proteinate with moderate chelation strength (Zn-Prot M) in broilers, three experiments were carried out to investigate the effects of y+LAT2 silencing and overexpression on zinc (Zn) absorption as Zn-Prot M and related gene expressions in broiler primary duodenal epithelial cells (BPDECs) contrasted with Zn sulfate (ZnSO4). In experiment 1, three pairs of siRNAs (si-494, si-834, and si-1153) targeting y+LAT2 were synthesized and transfected within the cells to identify the optimal siRNA for silencing y+LAT2 mRNA expression, and the data were analyzed by one-way ANOVA. In experiment 2, cells were incubated for 2\u2009h in either a DMEM\/F12 complete medium without Zn supplementation (Control) or the same medium enriched with 400\u2009\u03bcmol Zn\/liter as ZnSO4 or Zn-Prot M, with or without the only one effective siRNA targeting y+LAT2, and the data were analyzed by two-way ANOVA. In experiment 3, with or without the recombinant adenovirus plasmid encoding y+LAT2, cells were also treated for 2\u2009h with the same media as in experiment 2, and the data were analyzed by two-way ANOVA. The results demonstrated that in experiment 1, the si-494 was the only one effective (P\u2009&amp;lt;\u20090.0001) siRNA for inhibiting y+LAT2 mRNA expression. In experiment 2, y+LAT2 silencing reduced its own mRNA and protein abundances (P\u2009&amp;lt;\u20090.0001) by 46% and 14% and Zn absorption from Zn-Prot M by 34% (P\u2009&amp;lt;\u20090.05), with no effect (P\u2009&amp;gt;\u20090.05) on Zn absorption from ZnSO4. In experiment 3, y+LAT2 overexpression elevated its own mRNA and protein expressions by 949% and 18% (P\u2009&amp;lt;\u20090.0001) and Zn absorption from Zn-Prot M by 73% (P\u2009&amp;lt;\u20090.05), but didn\u2019t affect (P\u2009&amp;gt;\u20090.05) Zn absorption from ZnSO4. Additionally, Zn-Prot M upregulated (P\u2009&amp;lt;\u20090.05) the y+LAT2 protein expression compared to the Control and ZnSO4 by 12 to 17%, with no impact (P\u2009&amp;gt;\u20090.07) on the y+LAT2 mRNA expression, regardless of y+LAT2 silencing or overexpression. In conclusion, the y+LAT2 took part in promoting Zn absorption as Zn-Prot M in BPDECs.<\/jats:p>","DOI":"10.1093\/jas\/skaf466","type":"journal-article","created":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T12:39:27Z","timestamp":1767271167000},"source":"Crossref","is-referenced-by-count":2,"title":["The role of the y(+)L-type amino acid transporter 2 in promoting zinc absorption as zinc proteinate with moderate chelation strength in broiler primary duodenal epithelial cells"],"prefix":"10.1093","volume":"104","author":[{"given":"Chunyu","family":"Cao","sequence":"first","affiliation":[{"name":"Poultry Mineral Nutrition Laboratory, College of Animal Science and Technology, Yangzhou University , Yangzhou 225000,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yun","family":"Hu","sequence":"additional","affiliation":[{"name":"Poultry Mineral Nutrition Laboratory, College of Animal Science and Technology, Yangzhou University , Yangzhou 225000,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liang","family":"Huang","sequence":"additional","affiliation":[{"name":"Mineral Nutrition Research Division, State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences , Beijing 100193,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weiyun","family":"Zhang","sequence":"additional","affiliation":[{"name":"Poultry Mineral Nutrition Laboratory, College of Animal Science and Technology, Yangzhou University , Yangzhou 225000,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xi","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of Animal Science, North Carolina State University , Raleigh, NC 27695"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Wu","sequence":"additional","affiliation":[{"name":"Poultry Mineral Nutrition Laboratory, College of Animal Science and Technology, Yangzhou University , Yangzhou 225000,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tingting","family":"Li","sequence":"additional","affiliation":[{"name":"Poultry Mineral Nutrition Laboratory, College of Animal Science and Technology, Yangzhou University , Yangzhou 225000,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoyan","family":"Cui","sequence":"additional","affiliation":[{"name":"Poultry Mineral Nutrition Laboratory, College of Animal Science and Technology, Yangzhou University , Yangzhou 225000,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shengchen","family":"Wang","sequence":"additional","affiliation":[{"name":"Poultry Mineral Nutrition Laboratory, College of Animal Science and Technology, Yangzhou University , Yangzhou 225000,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liyang","family":"Zhang","sequence":"additional","affiliation":[{"name":"Mineral Nutrition Research Division, State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences , Beijing 100193,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xugang","family":"Luo","sequence":"additional","affiliation":[{"name":"Poultry Mineral Nutrition Laboratory, College of Animal Science and Technology, Yangzhou University , Yangzhou 225000,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2026,1,6]]},"reference":[{"key":"2026090801163439700_skaf466-B1","first-page":"32","volume-title":"The roles of amino acid chelates in animal nutrition","author":"Ashmead","year":"1993"},{"key":"2026090801163439700_skaf466-B2","doi-asserted-by":"publisher","first-page":"223","DOI":"10.2147\/ijn.S55974","article-title":"Interaction between nanoparticles generated by zinc chloride treatment and oxidative responses in rat liver","volume":"9","author":"Azzouz","year":"2014","journal-title":"Int. J. Nanomed"},{"issue":"8","key":"2026090801163439700_skaf466-B3","doi-asserted-by":"publisher","first-page":"559","DOI":"10.3390\/ani9080559","article-title":"Uptake of manganese from the manganese-lysine complex in primary chicken intestinal epithelial cells","volume":"9","author":"Bai","year":"2019","journal-title":"Animals. (Basel)"},{"issue":"4","key":"2026090801163439700_skaf466-B4","doi-asserted-by":"publisher","first-page":"457","DOI":"10.1007\/s00424-002-0840-y","article-title":"Adaptation of plasma membrane amino acid transport mechanisms to physiological demands","volume":"444","author":"Br\u00f6er","year":"2002","journal-title":"Pflugers Arch"},{"issue":"1","key":"2026090801163439700_skaf466-B5","doi-asserted-by":"publisher","first-page":"249","DOI":"10.1152\/physrev.00018.2006","article-title":"Amino acid transport across mammalian intestinal and renal epithelia","volume":"88","author":"Br\u00f6er","year":"2008","journal-title":"Physiol. Rev"},{"issue":"11","key":"2026090801163439700_skaf466-B6","doi-asserted-by":"publisher","first-page":"105755","DOI":"10.1016\/j.psj.2025.105755","article-title":"Effects of silencing or overexpression of ZIP3 and ZIP5 on zinc absorption as zinc sulfate or zinc proteinate and related gene expressions in primary duodenal epithelial cells of broilers","volume":"104","author":"Cao","year":"2025","journal-title":"Poult. Sci"},{"issue":"2","key":"2026090801163439700_skaf466-B7","doi-asserted-by":"publisher","first-page":"551","DOI":"10.1016\/j.jia.2022.08.051","article-title":"Establishment and evaluation of the primary cultured tibial osteoblast model of broiler chicks","volume":"22","author":"Cao","year":"2023","journal-title":"J. Integr. Agric"},{"issue":"1","key":"2026090801163439700_skaf466-B8","doi-asserted-by":"publisher","first-page":"237","DOI":"10.1111\/jpn.13260","article-title":"Kinetics of phosphorus absorption and expressions of related transporters in primary cultured duodenal epithelial cells of chick embryos","volume":"104","author":"Cao","year":"2020","journal-title":"J. Anim. Physiol. Anim. Nutr. (Berl)"},{"key":"2026090801163439700_skaf466-B9","doi-asserted-by":"publisher","first-page":"26","DOI":"10.3389\/fcimb.2016.00026","article-title":"Identification of biomarkers for footpad dermatitis development and wound healing","volume":"6","author":"Chen","year":"2016","journal-title":"Front. Cell. Infect. Microbiol"},{"key":"2026090801163439700_skaf466-B10","doi-asserted-by":"publisher","first-page":"771617","DOI":"10.3389\/fmicb.2021.771617","article-title":"Effects of dietary zinc sources on growth performance and gut health of weaned piglets","volume":"12","author":"Diao","year":"2021","journal-title":"Front. Microbiol"},{"issue":"3","key":"2026090801163439700_skaf466-B11","doi-asserted-by":"publisher","first-page":"102477","DOI":"10.1016\/j.psj.2023.102477","article-title":"The organic zinc with moderate chelation strength enhances the expression of related transporters in the jejunum and ileum of broilers","volume":"102","author":"Hu","year":"2023","journal-title":"Poult. Sci"},{"key":"2026090801163439700_skaf466-B12","doi-asserted-by":"crossref","DOI":"10.1016\/j.jia.2025.05.012","article-title":"Dietary Zn proteinate alleviates heat stress-induced intestinal integrity disruption possibly by promoting cell proliferation via related signaling pathways in the jejunum of broilers","author":"Hu","year":"2025","journal-title":"J. Integr. Agric"},{"key":"2026090801163439700_skaf466-B13","doi-asserted-by":"publisher","first-page":"952941","DOI":"10.3389\/fphys.2022.952941","article-title":"Organic zinc with moderate chelation strength enhances zinc absorption in the small intestine and expression of related transporters in the duodenum of broilers","volume":"13","author":"Hu","year":"2022","journal-title":"Front. Physiol"},{"key":"2026090801163439700_skaf466-B14","doi-asserted-by":"publisher","first-page":"skae204","DOI":"10.1093\/jas\/skae204","article-title":"Zinc proteinate with moderate chelation strength enhances zinc absorption by up-regulating the expression of zinc and amino acid transporters in primary cultured duodenal epithelial cells of broiler embryos","volume":"102","author":"Hu","year":"2024","journal-title":"J. Anim. Sci"},{"issue":"1","key":"2026090801163439700_skaf466-B15","doi-asserted-by":"publisher","first-page":"115","DOI":"10.1186\/s40104-024-01075-8","article-title":"Dietary Zn proteinate with moderate chelation strength alleviates heat stress-induced intestinal barrier function damage by promoting expression of tight junction proteins via the A20\/NF-\u03baB p65\/MMP-2 pathway in the jejunum of broilers","volume":"15","author":"Hu","year":"2024","journal-title":"J. Anim. Sci. Biotechnol"},{"issue":"6","key":"2026090801163439700_skaf466-B16","doi-asserted-by":"publisher","first-page":"2038","DOI":"10.2527\/jas.2008-1212","article-title":"Relative bioavailabilities of organic zinc sources with different chelation strengths for broilers fed a conventional corn-soybean meal diet","volume":"87","author":"Huang","year":"2009","journal-title":"J. Anim. Sci"},{"issue":"1-4","key":"2026090801163439700_skaf466-B17","doi-asserted-by":"publisher","first-page":"144","DOI":"10.1016\/j.anifeedsci.2012.10.010","article-title":"Relative bioavailabilities of organic zinc sources with different chelation strengths for broilers fed diets with low or high phytate content","volume":"179","author":"Huang","year":"2013","journal-title":"Anim. Feed Sci. Technol"},{"issue":"1","key":"2026090801163439700_skaf466-B18","doi-asserted-by":"publisher","first-page":"21","DOI":"10.1016\/j.molcel.2016.03.009","article-title":"Zinc-induced polymerization of killer-cell Ig-like receptor into filaments promotes its inhibitory function at cytotoxic immunological synapses","volume":"62","author":"Kumar","year":"2016","journal-title":"Mol. Cell"},{"issue":"7","key":"2026090801163439700_skaf466-B19","doi-asserted-by":"publisher","first-page":"2076","DOI":"10.1016\/s2095-3119(21)63771-1","article-title":"Effect of the gene silencing of phosphorus transporters on phosphorus absorption across primary cultured duodenal epithelial cell monolayers of chick embryos","volume":"21","author":"Li","year":"2022","journal-title":"J. Integr. Agric"},{"issue":"3","key":"2026090801163439700_skaf466-B20","doi-asserted-by":"publisher","first-page":"438","DOI":"10.4103\/1673-5374.153693","article-title":"Neuroprotective effects of SMADs in a rat model of cerebral ischemia\/reperfusion","volume":"10","author":"Liu","year":"2015","journal-title":"Neural Regen. Res"},{"issue":"4","key":"2026090801163439700_skaf466-B21","doi-asserted-by":"publisher","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method","volume":"25","author":"Livak","year":"2001","journal-title":"Methods"},{"issue":"9","key":"2026090801163439700_skaf466-B22","doi-asserted-by":"publisher","first-page":"3086","DOI":"10.3390\/nu13093086","article-title":"Zinc nutritional status and risk factors of elderly in the China adult chronic disease and nutrition surveillance 2015","volume":"13","author":"Lu","year":"2021","journal-title":"Nutrients"},{"issue":"12","key":"2026090801163439700_skaf466-B23","doi-asserted-by":"publisher","first-page":"5198","DOI":"10.1093\/jas\/sky375","article-title":"Organic iron absorption by in situ ligated jejunal and ileal loops of broilers","volume":"96","author":"Lu","year":"2018","journal-title":"J. Anim. Sci"},{"issue":"1","key":"2026090801163439700_skaf466-B24","doi-asserted-by":"publisher","first-page":"109","DOI":"10.3382\/japr.2007-00049","article-title":"Effect of different levels of dietary organic (bioplex) trace minerals on live performance of broiler chickens by growth phases","volume":"17","author":"Nollet","year":"2008","journal-title":"J. Appl. Poultry Res"},{"issue":"3","key":"2026090801163439700_skaf466-B25","doi-asserted-by":"publisher","first-page":"841","DOI":"10.1534\/genetics.111.136911","article-title":"Gene overexpression: uses, mechanisms, and interpretation","volume":"190","author":"Prelich","year":"2012","journal-title":"Genetics"},{"issue":"12","key":"2026090801163439700_skaf466-B26","doi-asserted-by":"publisher","first-page":"4547","DOI":"10.1039\/d0sc06680j","article-title":"Surmounting tumor resistance to metallodrugs by co-loading a metal complex and siRNA in nanoparticles","volume":"12","author":"Qiao","year":"2021","journal-title":"Chem. Sci"},{"issue":"3","key":"2026090801163439700_skaf466-B27","doi-asserted-by":"publisher","first-page":"381","DOI":"10.1080\/00071668.2013.787158","article-title":"Effect of intravenously injected zinc on tissue zinc and metallothionein gene expression of broilers","volume":"54","author":"Shen","year":"2013","journal-title":"Br. Poult. Sci"},{"key":"2026090801163439700_skaf466-B28","doi-asserted-by":"publisher","first-page":"skaf423","DOI":"10.1093\/jas\/skaf423","article-title":"Zinc proteinate alleviates thermal stress-induced damage to broiler jejunal organoid integrity and barrier function possibly by promoting cell proliferation via GPR39\/PLCbeta1-mediated PI3K\/AKT and MAPK signaling pathways","volume":"103","author":"Song","year":"2025","journal-title":"J. Anim. Sci"},{"key":"2026090801163439700_skaf466-B29","doi-asserted-by":"publisher","first-page":"5751","DOI":"10.2147\/ijn.S88109","article-title":"Endothelial cell-targeted pVEGF165 polyplex plays a pivotal role in inhibiting intimal thickening after vascular injury","volume":"10","author":"Tian","year":"2015","journal-title":"Int. J. Nanomed"},{"issue":"7","key":"2026090801163439700_skaf466-B30","doi-asserted-by":"publisher","first-page":"3174","DOI":"10.1021\/jacs.1c12750","article-title":"Hairpin-like siRNA-based spherical nucleic acids","volume":"144","author":"Vasher","year":"2022","journal-title":"J. Am. Chem. Soc"},{"key":"2026090801163439700_skaf466-B31","doi-asserted-by":"publisher","first-page":"595877","DOI":"10.3389\/fimmu.2020.595877","article-title":"Gene networks underlying the resistance of bifidobacterium longum to inflammatory factors","volume":"11","author":"Veselovsky","year":"2020","journal-title":"Front. Immunol"},{"key":"2026090801163439700_skaf466-B32","doi-asserted-by":"publisher","first-page":"733","DOI":"10.1016\/j.foodchem.2014.10.066","article-title":"Degradation and antioxidant activities of peptides and zinc-peptide complexes during in vitro gastrointestinal digestion","volume":"173","author":"Wang","year":"2015","journal-title":"Food Chem"},{"issue":"1","key":"2026090801163439700_skaf466-B33","doi-asserted-by":"publisher","first-page":"162","DOI":"10.1186\/s13287-017-0614-2","article-title":"Overexpression of c-met in bone marrow mesenchymal stem cells improves their effectiveness in homing and repair of acute liver failure","volume":"8","author":"Wang","year":"2017","journal-title":"Stem Cell Res. Ther"},{"issue":"1","key":"2026090801163439700_skaf466-B34","doi-asserted-by":"publisher","first-page":"224","DOI":"10.1007\/s12011-018-1460-5","article-title":"Comparison of inorganic and organically bound trace minerals on tissue mineral deposition and fecal excretion in broiler breeders","volume":"189","author":"Wang","year":"2019","journal-title":"Biol. Trace Elem. Res"},{"issue":"1","key":"2026090801163439700_skaf466-B35","doi-asserted-by":"publisher","first-page":"321","DOI":"10.1016\/j.aninu.2021.10.007","article-title":"Different copper sources and levels affect growth performance, copper content, carcass characteristics, intestinal microorganism and metabolism of finishing pigs","volume":"8","author":"Wen","year":"2022","journal-title":"Anim. Nutr"},{"key":"2026090801163439700_skaf466-B36","doi-asserted-by":"publisher","first-page":"1268","DOI":"10.3389\/fonc.2019.01268","article-title":"Cationic amino acid transporter-1-mediated arginine uptake is essential for chronic lymphocytic leukemia cell proliferation and viability","volume":"9","author":"Werner","year":"2019","journal-title":"Front. Oncol"},{"key":"2026090801163439700_skaf466-B37","doi-asserted-by":"publisher","first-page":"894572","DOI":"10.3389\/fnut.2022.894572","article-title":"The impact of zinc supplementation on critically ill patients with acute kidney injury: a propensity score matching analysis","volume":"9","author":"Xia","year":"2022","journal-title":"Front. Nutr"},{"key":"2026090801163439700_skaf466-B38","doi-asserted-by":"publisher","first-page":"9585047","DOI":"10.1155\/2020\/9585047","article-title":"The effects and mechanism of quercetin dietary supplementation in streptozotocin-induced hyperglycemic arbor Acre broilers","volume":"2020","author":"Ying","year":"2020","journal-title":"Oxid. Med. Cell. Longev"},{"issue":"8","key":"2026090801163439700_skaf466-B39","doi-asserted-by":"publisher","first-page":"1086","DOI":"10.1017\/s0007114517001040","article-title":"Organic zinc absorption by the intestine of broilers in vivo","volume":"117","author":"Yu","year":"2017","journal-title":"Br. J. Nutr"},{"issue":"10","key":"2026090801163439700_skaf466-B40","doi-asserted-by":"publisher","first-page":"2157","DOI":"10.3382\/ps.2009-00486","article-title":"Effects of zinc source and phytate on zinc absorption by in situ ligated intestinal loops of broilers","volume":"89","author":"Yu","year":"2010","journal-title":"Poult. Sci"},{"issue":"1-2","key":"2026090801163439700_skaf466-B41","doi-asserted-by":"publisher","first-page":"229","DOI":"10.1007\/s11010-014-2249-2","article-title":"L-arginine stimulates CAT-1-mediated arginine uptake and regulation of inducible nitric oxide synthase for the growth of chick intestinal epithelial cells","volume":"399","author":"Yuan","year":"2015","journal-title":"Mol. Cell. Biochem"},{"issue":"1","key":"2026090801163439700_skaf466-B42","doi-asserted-by":"publisher","first-page":"e10189","DOI":"10.1002\/btm2.10189","article-title":"Ultrasound-microbubble cavitation facilitates adeno-associated virus mediated cochlear gene transfection across the round-window membrane","volume":"6","author":"Zhang","year":"2021","journal-title":"Bioeng. Transl. Med"},{"key":"2026090801163439700_skaf466-B43","doi-asserted-by":"publisher","first-page":"857393","DOI":"10.3389\/fnut.2022.857393","article-title":"Comparison of the effects of inorganic or amino acid-chelated zinc on mouse myoblast growth in vitro and growth performance and carcass traits in growing-finishing pigs","volume":"9","author":"Zhang","year":"2022","journal-title":"Front. Nutr"},{"issue":"8","key":"2026090801163439700_skaf466-B44","first-page":"3159","article-title":"Isolation and identification of duodenal epithelial cells of broiler embryos in vitro and its establishment and evaluation of primary cultured absorption model","volume":"30","author":"Zhang","year":"2018","journal-title":"Chin. J. Anim. Nutri"},{"issue":"9","key":"2026090801163439700_skaf466-B45","doi-asserted-by":"publisher","first-page":"1699","DOI":"10.3390\/antiox11091699","article-title":"Mechanisms underlying the protective effect of maternal zinc (ZnSO4 or Zn-Gly) against heat stress-induced oxidative stress in chicken embryo","volume":"11","author":"Zhang","year":"2022","journal-title":"Antioxidants (Basel)"},{"key":"2026090801163439700_skaf466-B46","doi-asserted-by":"publisher","first-page":"1323","DOI":"10.2147\/ijn.S99692","article-title":"Hyaluronic acid reagent functional chitosan-PEI conjugate with AQP2-siRNA suppressed endometriotic lesion formation","volume":"11","author":"Zhao","year":"2016","journal-title":"Int. J. Nanomed"},{"key":"2026090801163439700_skaf466-B47","first-page":"6","article-title":"The influence of different lysine concentration on the cationic amino acid transporter mRNA expression of porcine IEC","volume":"26","author":"Zhi","year":"2010","journal-title":"Chin. Agric. Sci. Bull"}],"container-title":["Journal of Animal Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/jas\/advance-article-pdf\/doi\/10.1093\/jas\/skaf466\/66285510\/skaf466.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jas\/article-pdf\/doi\/10.1093\/jas\/skaf466\/66285510\/skaf466.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jas\/article-pdf\/doi\/10.1093\/jas\/skaf466\/66285510\/skaf466.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T05:16:38Z","timestamp":1788844598000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jas\/article\/doi\/10.1093\/jas\/skaf466\/8415893"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026]]},"references-count":47,"URL":"https:\/\/doi.org\/10.1093\/jas\/skaf466","relation":{},"ISSN":["0021-8812","1525-3163"],"issn-type":[{"value":"0021-8812","type":"print"},{"value":"1525-3163","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2026]]},"published":{"date-parts":[[2026]]},"article-number":"skaf466"}}